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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse IL-33 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-33 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Mouse
31.3 - 2000 pg/mL
1h 30m
= 5.3 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:29045903). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
Interleukin-33, IL-33, Il33
Mouse IL-33 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-33 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Interleukin-33, IL-33, Il33
Colorimetric
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Mouse
31.3 - 2000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 5.3 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Mouse Serum | n 5 | mean - | SD - | C.V. 5.9 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Mouse Serum | n 3 | mean - | SD - | C.V. 12.4 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 108 | Range 107 - 109 % |
Sample type Serum | Average % = 119 | Range 114 - 122 % |
Sample type EDTA Plasma | Average % = 115 | Range 110 - 118 % |
Sample type Heparin Plasma | Average % = 113 | Range 108 - 118 % |
Sample type Citrate plasma | Average % = 118 | Range 114 - 127 % |
Blue Ice
+4°C
+4°C
Mouse IL-33 ELISA Kit (ab213475) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-33 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-33 with 5.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-33 (Interleukin-33, also known as NF-HEV) is a proinflammatory cytokine member of the IL-1 family. It is widely expressed and functions by binding and signalling through the IL1RL1/ST2 receptor. IL-33 is expressed as a pro-protein; the antibodies in this kit were generated using the C-terminal mature form of IL-33.
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Terms & Conditions.
Mouse IL-33 standard curve comparison data.
Standard curve comparison between mouse IL-33 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.
Example mouse IL-33 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Example mouse IL-33 standard curve in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of spike IL-33 in mouse serum and plasma samples.
The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Interpolated concentrations of native IL-33 in mouse cell culture supernatant samples.
The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 50% and splenocyte supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 177 pg/mL in lung supernatant and 131 pg/mL in splenocyte supernatant.
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