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Mouse IL-33 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-33 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.


Images

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Sensitivity

= 5.3 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:29045903). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.

Alternative names

What's included?

1 x 96 Tests
Components
10X Mouse IL-33 Capture Antibody
1 x 600 µL
10X Mouse IL-33 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 5BR
1 x 6 mL
Mouse IL-33 Lyophilized Recombinant Protein (ab69516)
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NBS
1 x 20 mL
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Mouse IL-33 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse IL-33 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 5.3 pg/mL

Precision

Intra assay

Sample

Mouse Serum

n

5

mean

-

SD

-

C.V.

5.9

Inter assay

Sample

Mouse Serum

n

3

mean

-

SD

-

C.V.

12.4

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 108

Range

107 - 109 %

Sample type

Serum

Average %

= 119

Range

114 - 122 %

Sample type

EDTA Plasma

Average %

= 115

Range

110 - 118 %

Sample type

Heparin Plasma

Average %

= 113

Range

108 - 118 %

Sample type

Citrate plasma

Average %

= 118

Range

114 - 127 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse IL-33 ELISA Kit (ab213475) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-33 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-33 with 5.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-33 (Interleukin-33, also known as NF-HEV) is a proinflammatory cytokine member of the IL-1 family. It is widely expressed and functions by binding and signalling through the IL1RL1/ST2 receptor. IL-33 is expressed as a pro-protein; the antibodies in this kit were generated using the C-terminal mature form of IL-33.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475), expandable thumbnail

    Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475)

    Mouse IL-33 standard curve comparison data.

    Standard curve comparison between mouse IL-33 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.

  • Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475), expandable thumbnail

    Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475)

    Example mouse IL-33 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475), expandable thumbnail

    Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475)

    Example mouse IL-33 standard curve in Sample Diluent 25BS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475), expandable thumbnail

    Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475)

    Interpolated concentrations of spike IL-33 in mouse serum and plasma samples.

    The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475), expandable thumbnail

    Sandwich ELISA - Mouse IL-33 ELISA Kit (ab213475)

    Interpolated concentrations of native IL-33 in mouse cell culture supernatant samples.

    The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 50% and splenocyte supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 177 pg/mL in lung supernatant and 131 pg/mL in splenocyte supernatant.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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