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AB100711

Mouse IL-5 ELISA Kit

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(3 Publications)

Mouse IL-5 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-5 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF

2 Images
Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)

Representative standard curve using ab100711

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB100711)

Representative standard curve using ab100711

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1 pg/mL

Range

2.05 - 500 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get better reproducibility in only 90 minutes with Mouse IL-5 ELISA Kit (ab204523) from our SimpleStep ELISA® range.

The standard is affinity purified.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 104 %", "average": "= 95.76" }, { "sample": "Serum", "range": "82 - 102 %", "average": "= 93.44" }, { "sample": "Plasma", "range": "93 - 104 %", "average": "= 95.49" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100711-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100711-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100711-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100711-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IL-5", "number":"AB100711-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100711-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100711-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant mouse IL-5 Standard (lyophilized)", "number":"AB100711-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100711-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-5 Microplate (12 x 8 wells)", "number":"AB100711-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 10444455, PubMed : 1873482). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (PubMed : 3128631). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (By similarity).
See full target information Il5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 18:88 PubMed36184652

2022

Anti-asthmatic miR-224-5p inhibits the FHL1/MAPK pathway to repress airway smooth muscle cell proliferation in a murine model of asthma-like airway inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifang Zhuang,Yanjuan Zhou,Jiao Xu,Leying Pan

Clinical, cosmetic and investigational dermatology 14:1675-1683 PubMed34815684

2021

Gallic Acid Ameliorates Atopic Dermatitis-Like Skin Inflammation Through Immune Regulation in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Guohong Hu,Xiansheng Zhou

American journal of translational research 11:280-289 PubMed30787986

2019

Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Kong,Jia Yin
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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