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AB204523

Mouse IL-5 ELISA Kit

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(14 Publications )

Mouse IL-5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-5 with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
6 Images
ELISA - Mouse IL-5 ELISA Kit (AB204523)
  • ELISA

Supplier Data

ELISA - Mouse IL-5 ELISA Kit (AB204523)
Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)

Comparison of secreted Il-5 in unstimulated and PHA/PMA stimulated EL-4 (IL-2) Cells.

EL-4 (IL-2) cells were grown in the absence (unstimulated) or presence of 10 ug/mL PHA and 10 ng/mL PMA (stimulated) for 48 hours. IL-5 was measured in undiluted cell culture supernatants of unstimulated and PHA/PMA stimulated EL-4 (IL-2). Measured values were interpolated from the IL-5 Standard Curve diluted in Sample Diluent NS. Mean of duplicate values +/-SD are graphed : 1.05 pg/mL unstimulated, 535 pg/mL stimulated.

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)

Linearity of dilution of the assay.

Interpolated concentrations of IL-5 in mouse cell culture media. The concentrations of IL-5 were measured in duplicate and interpolated from the IL-5 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 642 pg/mL in mouse EL-4 (IL-2) PHA/PMA-stimulated supernatant.

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)

Example of IL-5 standard curve prepared in Sample Diluent 25BP.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)

Mouse IL-5 standard curve comparison data.

Standard curve comparison between mouse IL-5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 5-fold increase in sensitivity.

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-5 ELISA Kit (AB204523)

Example of IL-5 standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.2 pg/mL

Range

3.9 - 250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-5 ELISA Kit ab204523 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-5 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.2 pg/mL.

How the assay works

Mouse IL-5 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-5 ELISA Kit ab204523 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-5 antibody [EPR16614-25] - BSA and Azide free (Capture) ab242707
- Anti-IL-5 antibody [EPR16614-120] - BSA and Azide free (Detector) ab242707

Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 107 %", "average": "= 106" }, { "sample": "Serum", "range": "101 - 107 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "85 - 91 %", "average": "= 88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab204523-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB204523-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB204523-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB204523-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB204523-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB204523-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB204523-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB204523-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB204523-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-5 Lyophilized Recombinant Protein", "number":"AB204523-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-5 Capture Antibody", "number":"AB204523-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-5 Detector Antibody", "number":"AB204523-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 10444455, PubMed : 1873482). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (PubMed : 3128631). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (By similarity).
See full target information Il5

Alternative Names

Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 105:e70142 PubMed40556500

2025

Apelin-13 Protects Against Airway Inflammation, Oxidative Stress, and Epithelial-Mesenchymal Transition in a Chronic Asthma Model.

Applications

Unspecified application

Species

Unspecified reactive species

Huifeng Sun,Ai Wang,Nan Shi

Cells 13: PubMed39451220

2024

Novel TSPO Ligand 2-Cl-MGV-1 Can Counteract Lipopolysaccharide Induced Inflammatory Response in Murine RAW264.7 Macrophage Cell Line and Lung Models.

Applications

Unspecified application

Species

Unspecified reactive species

Fadi Obeid,Meygal Kahana,Baraah Dahle,Sheelu Monga,Yaniv Zohar,Abraham Weizman,Moshe Gavish

Allergologia et immunopathologia 52:91-96 PubMed38970271

2024

Jolkinolide B attenuates allergic airway inflammation and airway remodeling in asthmatic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Lin,Chao Xu,Jintong Ge,Hua Wu,Qi Wang

Cell death & disease 15:400 PubMed38849380

2024

Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxiang Wu,Pin Wang,Xinyu Xie,Xiaoqi Yang,Shuangmei Tang,Jiping Zhao,Tian Liu,Junfei Wang,Jintao Zhang,Tongliang Xia,Xin Feng

Allergologia et immunopathologia 51:164-174 PubMed37422794

2023

Inhibition of CREB promotes glucocorticoids action on airway inflammation in pediatric asthma by promoting ferroptosis of eosinophils.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Yu,Yiping Yu,Yingyu Ma,Guoqing Chen

Allergologia et immunopathologia 51:146-153 PubMed36617834

2023

Chryseriol attenuates the progression of OVA-induced asthma in mice through NF-κB/HIF-1α and MAPK/STAT1 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shangyao Mo,Hao Deng,Yong Xie,Lixia Yang,Lili Wen

Molecular medicine reports 25: PubMed35234263

2022

SIRT3 regulates bronchial epithelium apoptosis and aggravates airway inflammation in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Song,Jinxiang Wang

Pharmaceutical biology 60:326-333 PubMed35167426

2022

Tanshinone IIA alleviates ovalbumin-induced allergic rhinitis symptoms by inhibiting Th2 cytokine production and mast cell histamine release in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Chen,Liping Shao,Yong Li,Mian Dai,He Liu,Nan Xiang,Hui Chen

Experimental and therapeutic medicine 23:193 PubMed35126696

2022

Lidocaine relieves murine allergic rhinitis by regulating the NF-κB and p38 MAPK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiang,Zhen Yang,Qiang Zhou

International archives of allergy and immunology 183:375-388 PubMed34942624

2021

Mechanism of miR-181a-5p in Regulatory T/T-Helper 17 Immune Imbalance and Asthma Development in Mice with Allergic Rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyun He,Yujuan Chen,Xiaoer Chen,Binfan Yuan
View all publications
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