JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326903

Mouse IL-5 ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse IL-5 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IL-5 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.69 - 500 pg/ml with a sensitivity of 0.37 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Il-5, Il5, Interleukin-5, IL-5, B-cell growth factor II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, BCGF-II, TRF

5 Images
Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)

Dynamic range comparison between the Chemiluminescent Human IL-5 SimpleStep ELISA, colorimetric Human IL-5 SimpleStep ELISA (ab204523), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)

Standard curve comparison between the Chemiluminescent Human IL-5 SimpleStep ELISA and colorimetric Human IL-5 SimpleStep ELISA (ab204523) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)

Concentrations of native IL-5 were measured in duplicate in 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BP.

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)

Comparison of secreted Il-5 in unstimulated and PHA/PMA stimulated EL-4 (IL-2) Cells.

EL-4 (IL-2) cells were grown in the absence (unstimulated) or presence of 10 ug/mL PHA and 10 ng/mL PMA (stimulated) for 48 hours. IL-5 was measured in undiluted cell culture supernatants of unstimulated and PHA/PMA stimulated EL-4 (IL-2). Measured values were interpolated from the IL-5 Standard Curve diluted in Sample Diluent NS. Mean of duplicate values +/-SD are graphed : 1.05 pg/mL unstimulated, 535 pg/mL stimulated.

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-5 ELISA Kit, Chemiluminescent (AB326903)

Linearity of dilution of the assay.

Interpolated concentrations of IL-5 in mouse cell culture media. The concentrations of IL-5 were measured in duplicate and interpolated from the IL-5 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 642 pg/mL in mouse EL-4 (IL-2) PHA/PMA-stimulated supernatant.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, Heparin Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.37 pg/mL

Range

0.69 - 500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse IL-5 ELISA Kit, Chemiluminescent (ab326903) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IL-5 in Cell culture supernatant, Serum, Heparin Plasma with a sensitivity of 0.37 pg/mL.

How the assay works

Mouse IL-5 ELISA Kit, Chemiluminescent (ab326903) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-5 (ab326903) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-5 antibody [EPR16614-25] - BSA and Aside free (Capture) ab242456
- Anti-IL-5 antibody [EPR16614-120] - BSA and Aside free (Detector) ab242707
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.1" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 107 %", "average": "= 107" }, { "sample": "Serum", "range": "101 - 107 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "85 - 91 %", "average": "= 91" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326903-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Mouse IL-5 Detector Antibody", "number":"AB326903-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326903-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326903-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326903-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-5 Capture Antibody", "number":"AB326903-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326903-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB326903-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-5 Lyophilized Recombinant Protein", "number":"AB326903-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326903-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326903-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326903-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 10444455, PubMed : 1873482). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (PubMed : 3128631). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (By similarity).
See full target information Il5
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com