JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB222503

Mouse IL-6 ELISA Kit

5

(2 Reviews)

|

(132 Publications)

Mouse IL-6 ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantification of mouse interleukin-6 (IL-6) with a sensitivity of 11.3 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility - works with serum, plasma, and cell culture supernatants
- Cited in over 130 publications

View Alternative Names

Il-6, Il6, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1

8 Images
Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Example of mouse IL-6 standard curve.

The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of native IL-6 in mouse serum and plasma samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 39 pg/mL in serum, 42 pg/mL in plasma (heparin), 43 pg/mL in plasma (EDTA), and 32 pg/mL in plasma (citrate).

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of spiked IL-6 in mouse serum and plasma samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Linearity of dilution.

Native IL-6 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-6 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of native IL-6 in mouse cell culture supernatant samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated RAW264.7 cell culture supernatant 1 : 50 and stimulated spleen cell culture supernatant 1 : 16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 26,793 pg/mL in stimulated RAW264.7 cell culture supernatant, and 15,127 pg/mL in stimulated spleen cell culture supernatant.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Example of mouse IL-6 standard curve. The IL-6 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 11.3 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

Mouse IL-6 ELISA Kit ab222503 is a rapid single-wash 90-min ELISA kit to measure cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 11.3 pg/mL.

How the assay works

SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant mouse IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Mouse IL-6 ELISA Kit ab222503 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse IL-6 ELISA kit ab222503

Mouse IL-6 ELISA kit ab222503 has been used to assess inflammation in different physio-pathological contexts such as liver disease(1), diabetes(2), and aging(3).

References:

(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Lili Shi et al. 2024, PMID: 38169591,
(3)Yang Bao et al. 2023, PMID: 37954634

Save your precious samples

Our 384-well Mouse IL-6 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse IL-6 Antibody Pair - BSA and Azide free ab241663
- Anti-IL-6 antibody [EPR16610-380] - BSA and Azide free (Capture) ab242490
- Anti-IL-6 antibody [EPR16610-318] - BSA and Azide free (Detector) ab242739

Related and recommended products

Mouse IL-6 ELISA kit ab222503 is commonly used to measure IL-6 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Mouse TNF alpha ELISA kit ab208348
- Mouse IL-10 ELISA kit ab255729
- Mouse IL-1 beta ELISA kit ab197742

In glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.
Explore our comprehensive range of glioblastoma research solutions here: https://www.abcam.com/en-us/products/product-recommendations/glioblastoma-research-tools

In Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.
Explore our extensive Alzheimer's research solutions here: https://www.abcam.com/en-us/products/product-recommendations/alzheimers-disease-tools

Interleukin 6 (IL-6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL-6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Spleen CCS", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Spleen CCS", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "103 - 114 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "109 - 116 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "95 - 103 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "96 - 102 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "89 - 112 %", "average": "= 100" } ]
style
left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab222503-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB222503-CMP06" }, { "size":"2 x 1 Vial", "name":"Mouse IL-6 Lyophilized Recombinant Protein", "number":"AB222503-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB222503-CMP10" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-6 Capture Antibody", "number":"AB222503-CMP02" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB222503-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB222503-CMP01" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB222503-CMP09" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-6 Detector Antibody", "number":"AB222503-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB222503-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB222503-CMP11" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab222503-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB222503-CMP06" }, { "size":"2 x 10 Vial", "name":"Mouse IL-6 Lyophilized Recombinant Protein", "number":"AB222503-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB222503-CMP10" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB222503-CMP04" }, { "size":"10 x 600 µL", "name":"10X Mouse IL-6 Capture Antibody", "number":"AB222503-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB222503-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB222503-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB222503-CMP09" }, { "size":"10 x 600 µL", "name":"10X Mouse IL-6 Detector Antibody", "number":"AB222503-CMP03" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB222503-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab222503-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB222503-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-6 Lyophilized Recombinant Protein", "number":"AB222503-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB222503-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB222503-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-6 Capture Antibody", "number":"AB222503-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB222503-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB222503-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-6 Detector Antibody", "number":"AB222503-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB222503-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB222503-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (PubMed : 8910279). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (PubMed : 27893700).. IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed : 16990136, PubMed : 27893700). Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable).. Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed : 17075861). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed : 11786910). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed : 28402851). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (PubMed : 11113088). Also acts as a myokine (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed : 11113088, PubMed : 8910279). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed : 15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).
See full target information Il6
style
left-column

Publications (132)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:505-519 PubMed37902450

2024

Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei Xu

Journal of translational autoimmunity 8:100228 PubMed38225946

2024

ANKRD22 aggravates sepsis-induced ARDS and promotes pulmonary M1 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Zhang,Yao Liu,Xiao-Long Zhang,Yun Sun,Zhong-Hua Lu

NPJ systems biology and applications 10:5 PubMed38218856

2024

The antidepressive mechanism of Longya Lilium combined with Fluoxetine in mice with depression-like behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Huina Ma,Hehua Huang,Chenyu Li,Shasha Li,Juefang Gan,Chunrong Lian,Yanwu Ling

International journal of biological sciences 20:585-605 PubMed38169591

2024

SIRT5-related lysine demalonylation of GSTP1 contributes to cardiomyocyte pyroptosis suppression in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Can Wei,Meixin Shi,Shiyun Dong,Zhitao Li,Bingbing Zhao,Dan Liu,Guopeng Li,Jie Cen,Ligen Yu,Xiao Liang,Lili Shi

AMB Express 13:125 PubMed37943403

2023

Anti-Cryptosporidium oocysts polyclonal antibodies for cryptosporidiosis diagnosis and protection.

Applications

Unspecified application

Species

Unspecified reactive species

Alyaa Farid,Silvia Aiad,Gehan Safwat

Journal of inflammation research 16:5095-5109 PubMed38026263

2023

LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi Chen

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 18:628-639 PubMed37919457

2023

GPNMB Ameliorates Neuroinflammation Via the Modulation of AMPK/NFκB Signaling Pathway After SAH in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Yuansheng Zhang,Qixiong Lu,Li Lei,Jingshu Du,Xiaoyang Lu

Biomedical reports 19:100 PubMed37954634

2023

Dexmedetomidine attenuates the neuroinflammation and cognitive dysfunction in aged mice by targeting the SNHG14/miR‑340/NF‑κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangbao He,Hongwei Ni,Kai Wang,Hongmei Gao,Yu Li,Jiaqiu Gu,Xin Ni,Zhiming Wang,Yang Bao

Neurotoxicity research 41:615-626 PubMed37782433

2023

Effect of Cannabidiol in LPS-Induced Toxicity in Astrocytes: Possible Role for Cannabinoid Type-1 Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Hind Ibork,Sara El Idrissi,Simo Siyanda Zulu,Robert Miller,Lhoussain Hajji,Annabelle Manalo Morgan,Khalid Taghzouti,Oualid Abboussi

Frontiers in endocrinology 14:1189192 PubMed37818088

2023

High blood galectin-3 level associated with risk of frailty in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Ji,Zhaoshun Jiang,Yixuan Qiu,Jiaming Yu,Yan Zhang,Jiaofeng Wang,Bo Ye,Yuxin Huang,Weidong Gu,Yiqin Huang,Jie Chen,Zhijun Bao
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column

Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB100712

Mouse IL-6 ELISA Kit

elisa-kits

mouse-il-6-elisa-kit-ab100712

0

(0 reviews)

Alternative Product
Assay Kits

AB100713

Mouse IL-6 ELISA Kit

elisa-kits

mouse-il-6-elisa-kit-ab100713

4

(1 reviews)

Alternative Product
Assay Kits

AB46100

Mouse IL-6 ELISA Kit

elisa-kits

mouse-il-6-elisa-kit-ab46100

4

(1 reviews)

Alternative Product
Assay Kits

AB213749

Mouse IL-6 Matched Antibody Pair Kit

matched-antibody-pair-kits

mouse-il-6-matched-antibody-pair-kit-ab213749

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com