Mouse IL-6 ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantification of mouse interleukin-6 (IL-6) with a sensitivity of 11.3 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility - works with serum, plasma, and cell culture supernatants
- Cited in over 130 publications
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- Biomolecules & biomedicine 24:505-5192024Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei XuPubMed 37902450
- Journal of translational autoimmunity 8:1002282024ANKRD22 aggravates sepsis-induced ARDS and promotes pulmonary M1 macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi Zhang,Yao Liu,Xiao-Long Zhang,Yun Sun,Zhong-Hua LuPubMed 38225946
- NPJ systems biology and applications 10:52024The antidepressive mechanism of Longya Lilium combined with Fluoxetine in mice with depression-like behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuina Ma,Hehua Huang,Chenyu Li,Shasha Li,Juefang Gan,Chunrong Lian,Yanwu LingPubMed 38218856
- International journal of biological sciences 20:585-6052024SIRT5-related lysine demalonylation of GSTP1 contributes to cardiomyocyte pyroptosis suppression in diabetic cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCan Wei,Meixin Shi,Shiyun Dong,Zhitao Li,Bingbing Zhao,Dan Liu,Guopeng Li,Jie Cen,Ligen Yu,Xiao Liang,Lili ShiPubMed 38169591
- AMB Express 13:1252023Anti-Cryptosporidium oocysts polyclonal antibodies for cryptosporidiosis diagnosis and protection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyaa Farid,Silvia Aiad,Gehan SafwatPubMed 37943403
- Journal of inflammation research 16:5095-51092023LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi ChenPubMed 38026263
- Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 18:628-6392023GPNMB Ameliorates Neuroinflammation Via the Modulation of AMPK/NFκB Signaling Pathway After SAH in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Li,Yuansheng Zhang,Qixiong Lu,Li Lei,Jingshu Du,Xiaoyang LuPubMed 37919457
- Biomedical reports 19:1002023Dexmedetomidine attenuates the neuroinflammation and cognitive dysfunction in aged mice by targeting the SNHG14/miR‑340/NF‑κB axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangbao He,Hongwei Ni,Kai Wang,Hongmei Gao,Yu Li,Jiaqiu Gu,Xin Ni,Zhiming Wang,Yang BaoPubMed 37954634
- Neurotoxicity research 41:615-6262023Effect of Cannabidiol in LPS-Induced Toxicity in Astrocytes: Possible Role for Cannabinoid Type-1 Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHind Ibork,Sara El Idrissi,Simo Siyanda Zulu,Robert Miller,Lhoussain Hajji,Annabelle Manalo Morgan,Khalid Taghzouti,Oualid AbboussiPubMed 37782433
- Frontiers in endocrinology 14:11891922023High blood galectin-3 level associated with risk of frailty in aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXueying Ji,Zhaoshun Jiang,Yixuan Qiu,Jiaming Yu,Yan Zhang,Jiaofeng Wang,Bo Ye,Yuxin Huang,Weidong Gu,Yiqin Huang,Jie Chen,Zhijun BaoPubMed 37818088
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 11.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (PubMed:8910279). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (PubMed:27893700). IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed:23045607). Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed:16990136, PubMed:27893700). Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed:17075861). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed:11786910). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed:28402851). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (PubMed:11113088). Also acts as a myokine (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed:11113088, PubMed:8910279). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed:15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).
Alternative names
Il-6, Il6, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1
What's included?
Recommended products
Mouse IL-6 ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantification of mouse interleukin-6 (IL-6) with a sensitivity of 11.3 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility - works with serum, plasma, and cell culture supernatants
- Cited in over 130 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 11.3 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Spleen CCS | n 5 | C.V. 3.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Spleen CCS | n 3 | C.V. 3.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 107 | Range 103 - 114 % |
Sample type EDTA Plasma | Average % = 112 | Range 109 - 116 % |
Sample type Heparin Plasma | Average % = 98 | Range 95 - 103 % |
Sample type Citrate plasma | Average % = 99 | Range 96 - 102 % |
Sample type Cell culture media | Average % = 100 | Range 89 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse IL-6 ELISA Kit ab222503 is a rapid single-wash 90-min ELISA kit to measure cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 11.3 pg/mL.
How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
Mouse IL-6 ELISA Kit ab222503 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse IL-6 ELISA kit ab222503
Mouse IL-6 ELISA kit ab222503 has been used to assess inflammation in different physio-pathological contexts such as liver disease(1), diabetes(2), and aging(3).
References:
(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Lili Shi et al. 2024, PMID: 38169591,
(3)Yang Bao et al. 2023, PMID: 37954634
Save your precious samples
Our 384-well Mouse IL-6 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse IL-6 Antibody Pair - BSA and Azide free Mouse IL-6 Antibody Pair - BSA and Azide free ab241663
- Anti-IL-6 antibody [EPR16610-380] - BSA and Azide free (Capture) Anti-IL-6 antibody [EPR16610-380] - BSA and Azide free (Capture) ab242490
- Anti-IL-6 antibody [EPR16610-318] - BSA and Azide free (Detector) Anti-IL-6 antibody [EPR16610-318] - BSA and Azide free (Detector) ab242739
Related and recommended products
Mouse IL-6 ELISA kit ab222503 is commonly used to measure IL-6 as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Mouse TNF alpha ELISA kit Mouse TNF alpha ELISA Kit ab208348
- Mouse IL-10 ELISA kit Mouse IL-10 ELISA Kit ab255729
- Mouse IL-1 beta ELISA kit Mouse IL-1 beta ELISA Kit ab197742
In glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.
Explore our comprehensive range of glioblastoma research solutions here: https://www.abcam.com/en-us/products/product-recommendations/glioblastoma-research-tools
In Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.
Explore our extensive Alzheimer's research solutions here: https://www.abcam.com/en-us/products/product-recommendations/alzheimers-disease-tools
Interleukin 6 (IL-6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL-6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance
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8 product images
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
Example of mouse IL-6 standard curve.
The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
Example of mouse IL-6 standard curve. The IL-6 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
Interpolated concentrations of spiked IL-6 in mouse serum and plasma samples.
The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
Interpolated concentrations of native IL-6 in mouse serum and plasma samples.
The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 39 pg/mL in serum, 42 pg/mL in plasma (heparin), 43 pg/mL in plasma (EDTA), and 32 pg/mL in plasma (citrate).
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
Interpolated concentrations of native IL-6 in mouse cell culture supernatant samples.
The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated RAW264.7 cell culture supernatant 1:50 and stimulated spleen cell culture supernatant 1:16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 26,793 pg/mL in stimulated RAW264.7 cell culture supernatant, and 15,127 pg/mL in stimulated spleen cell culture supernatant.
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-6 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
Linearity of dilution.
Native IL-6 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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