AB203360

Mouse IL-6 R alpha ELISA Kit

Recombinant
SimpleStep
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(7 Publications)

Mouse IL-6 R alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-6 R alpha with a sensitivity of 12.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CD126, Il6r, Il6ra, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1

5 Images

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Comparison of secreted IL-6 R alpha in unstimulated and PMA/PHA-stimulated J774A.1 Cells.

J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. IL-6 R alpha was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the IL-6 R alpha Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed, 568 pg/mL unstimulated, 849 pg/mL stimulated, and undetectable in media.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Interpolated concentrations of IL-6 R alpha in mouse plasma (heparin), and J774A.1 cell culture supernatant.

The concentrations of IL-6 R alpha were measured in duplicate and interpolated from the IL-6 R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 R alpha concentration was determined to be 17,650 pg/mL in mouse plasma (heparin), and 849 pg/mL in J774A.1 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Example of IL-6 R alpha standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Mouse IL-6 R alpha standard curve comparison data.

Standard curve comparison between mouse IL-6 R alpha SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 5-fold increase in sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Interpolated concentrations of IL-6 R alpha in mouse serum, plasma (citrate), and plasma (EDTA).

The concentrations of IL-6 R alpha were measured in duplicate and interpolated from the IL-6 R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 R alpha concentration was determined to be 20.9 ng/mL in mouse serum, 19.1 ng/mL in mouse plasma (Citrate), and 17.1 ng/mL in mouse plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 12.6 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IL-6 R alpha ELISA Kit (ab203360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 R alpha protein in cell culture supernatant, serum, urine, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-6 R alpha with 12.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-6 Receptor (IL-6R, IL-6 Receptor), also known as Interleukin-6 Receptor subunit alpha and membrane glycoprotein 80, is part of a receptor that binds Interleukin-6 (IL-6). IL-6 Receptor weakly binds IL-6 with a low affinity, and then is presented to gp130 to facilitate transition into a high affinity signaling competent hexamer. Activation may lead to regulation of the immune response, acute-phase reactions and hematopoiesis. The production of IL-6 Receptor has been implicated in the pathogenesis of many diseases, including multiple myeloma, auto immune diseases and prostate cancer.IL-6 Receptor has two isoforms. The first is a transmembrane protein in which the N-terminal extracellular portion can be released by proteolytic cleavage. The second isoform is a secreted form that lacks the transmembrane and intracellular portions. This assay is directed at the extracellular portion of IL-6 Receptor and detects both the soluble and membrane bound forms.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" } ]

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Recovery

[ { "sample": "Serum", "range": "103 - 106 %", "average": "= 105" }, { "sample": "Urine", "range": "80 - 89 %", "average": "= 85" }, { "sample": "Cell culture media", "range": "84 - 93 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "100 - 107 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "102 - 103 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "101 - 105 %", "average": "= 103" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab203360-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB203360-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB203360-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-6 R alpha Detector Antibody", "number":"AB203360-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB203360-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB203360-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB203360-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB203360-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB203360-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-6 R alpha Capture Antibody", "number":"AB203360-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-6 R alpha Lyophilized Recombinant Protein", "number":"AB203360-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-6R also known as the IL-6 receptor or IL-6R protein is a transmembrane protein with an approximate mass of 80-90 kDa. This receptor is found on the surface of various cells including hepatocytes leukocytes and immune cells like T-cells and macrophages. IL-6R binds to interleukin-6 (IL-6) a cytokine and plays an important role in immune response regulation. The receptor exists in both membrane-bound and soluble forms each contributing to diverse signaling events.

Biological function summary

IL-6 receptor function involves mediating the effects of IL-6 through its interaction with glycoprotein 130 (gp130) forming a signaling complex. This complex initiates intracellular pathways that lead to gene expression changes necessary for immune responses inflammation and hematopoiesis. When IL-6 binds IL-6R the complex formation with gp130 triggers downstream signaling that impacts cell growth differentiation and survival.

Pathways

IL-6R is central to the JAK/STAT signaling pathway one of the two main pathways activated by IL-6 signaling. It's also involved in the MAPK cascade contributing to cellular responses such as proliferation and differentiation. Through these pathways IL-6R interacts with other proteins like JAK kinases and STAT transcription factors coordinating cellular responses to external signals.

IL-6R is associated with inflammatory diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis overexpression of IL-6R leads to persistent inflammation and joint damage. In cancer IL-6/IL-6R signaling promotes tumorigenesis and cancer cell survival through interactions with proteins like STAT3. Understanding these connections aids in developing therapeutic interventions targeting IL-6R in various pathological conditions.

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Product protocols

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Target data

Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.. Interleukin-6 receptor subunit alpha. Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed : 27893700).. Soluble interleukin-6 receptor subunit alpha. Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed : 11113088). The IL6 : sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (By similarity). In complex with IL6, is required for induction of VEGF production (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed : 11113088). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed : 28402851).

See full target information Il6ra

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 19:836 PubMed39696421

2024

CCL4L2 participates in tendinopathy progression by promoting macrophage inflammatory responses: a single-cell analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Junxiang Xu,Minzhe Zheng,Zongxian Feng,Qiji Lin

Bioengineered 12:9902-9913 PubMed34895052

2021

MicroRNA miR-27b-3p regulate microglial inflammation response and cell apoptosis by inhibiting A20 (TNF-α-induced protein 3).

Applications

Unspecified application

Species

Unspecified reactive species

Liping Li,Chao Qi,Yuanyuan Liu,Youliang Shen,Xia Zhao,Han Qin,Yi Zhang,Tengbo Yu

Chemical & pharmaceutical bulletin 69:529-536 PubMed34078799

2021

MiR-135a Protects against Myocardial Injury by Targeting TLR4.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Feng,Bing Xie,Zhuoqi Zhang,Jun Yan,Mingyue Cheng,Yafeng Zhou

American journal of translational research 13:4632-4643 PubMed34150043

2021

The protective role of miR-132 targeting HMGA2 through the PI3K/AKT pathway in mice with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xichang Liu,Haitao Wang,Jiawei Bei,Jun Zhao,Ge Jiang,Xiuhong Liu

Immunity 54:235-246.e5 PubMed33357409

2020

The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ashraf S Yousif,Larance Ronsard,Pankaj Shah,Tatsushi Omatsu,Maya Sangesland,Thalia Bracamonte Moreno,Evan C Lam,Vladimir D Vrbanac,Alejandro B Balazs,Hans-Christian Reinecker,Daniel Lingwood

Journal of neuroimmunology 319:71-79 PubMed29685293

2018

Anti-IL-6 receptor antibody improves pain symptoms in mice with experimental autoimmune encephalomyelitis.

Applications

ELISA

Species

Mouse

Kenichi Serizawa,Haruna Tomizawa-Shinohara,Mayu Magi,Kenji Yogo,Yoshihiro Matsumoto

PloS one 11:e0146463 PubMed26745884

2016

Radiation Inhibits Interleukin-12 Production via Inhibition of C-Rel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jung Lee,Seo Jin Lee,Ji-Hye Kim,Kyoung-Jin Kim,Seung-Hyun Yang,Keun-Yeong Jeong,Jinsil Seong

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse IL-6 R alpha ELISA Kit

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit (AB203360)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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