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AB326900

Mouse IL-6 R alpha ELISA Kit, Chemiluminescent

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Mouse IL-6 R alpha ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IL-6 R alpha with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 5.49 - 4000 pg/ml with a sensitivity of 3.69 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

CD126, Il6r, Il6ra, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1

5 Images
Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)

Interpolated concentrations of IL-6 R alpha in mouse serum, plasma (citrate), and plasma (EDTA).

The concentrations of IL-6 R alpha were measured in duplicate and interpolated from the IL-6 R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 R alpha concentration was determined to be 20.9 ng/mL in mouse serum, 19.1 ng/mL in mouse plasma (Citrate), and 17.1 ng/mL in mouse plasma (EDTA).

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)

Comparison of secreted IL-6 R alpha in unstimulated and PMA/PHA-stimulated J774A.1 Cells.

J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. IL-6 R alpha was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the IL-6 R alpha Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed, 568 pg/mL unstimulated, 849 pg/mL stimulated, and undetectable in media.

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)

Interpolated concentrations of IL-6 R alpha in mouse plasma (heparin), and J774A.1 cell culture supernatant.

The concentrations of IL-6 R alpha were measured in duplicate and interpolated from the IL-6 R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 R alpha concentration was determined to be 17,650 pg/mL in mouse plasma (heparin), and 849 pg/mL in J774A.1 cell culture supernatant.

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)

Standard curve comparison between the Chemiluminescent Mouse IL-6 R alpha SimpleStep ELISA and colorimetric Mouse IL-6 R alpha SimpleStep ELISA (ab203360) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (AB326900)

Dynamic range comparison between the Chemiluminescent Mouse IL-6 R alpha SimpleStep ELISA, colorimetric Mouse IL-6 R alpha SimpleStep ELISA (ab203360), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Luminescent

Sample types

Serum, Urine, Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.69 pg/mL

Range

5.49 - 4000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (ab326900) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IL-6 R alpha in Serum, Urine, Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma with a sensitivity of 3.69 pg/mL.

How the assay works

Mouse IL-6 R alpha ELISA Kit, Chemiluminescent (ab326900) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-6 R alpha (ab326900) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-6R antibody [EPR17264-273] - BSA and Azide free (Capture) ab242471
- Anti-IL-6R antibody [EPR17263-113] - BSA and Azide free (Detector) ab242722
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Precision

[ { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.8" }, { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 12" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 106 %", "average": "= 106" }, { "sample": "Urine", "range": "80 - 89 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "84 - 93 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "100 - 107 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "102 - 103 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "101 - 105 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.. Interleukin-6 receptor subunit alpha. Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed : 27893700).. Soluble interleukin-6 receptor subunit alpha. Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed : 11113088). The IL6 : sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (By similarity). In complex with IL6, is required for induction of VEGF production (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed : 11113088). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed : 28402851).
See full target information Il6ra
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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