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AB100714

Mouse IL-7 ELISA Kit

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(2 Publications )

Mouse IL-7 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-7 in Mouse in Biofluids, Cell Culture Media samples.
2 Images
Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)

Representative standard curve using ab100714

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)
  • sELISA

Unknown

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB100714)

Representative standard curve using ab100714

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-7 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-7 in serum, plasma and cell culture supernates.

This assay employs an antibody specific for mouse IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision & recovery information

Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 105 %", "average": "= 94.58" }, { "sample": "Serum", "range": "82 - 103 %", "average": "= 91.59" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 92.66" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100714-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100714-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100714-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100714-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-7 Microplate (12 x 8 wells)", "number":"AB100714-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100714-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100714-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant mouse IL-7 Standard (lyophilized)", "number":"AB100714-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-7", "number":"AB100714-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"440X HRP-Streptavidin Concentrate", "number":"AB100714-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
Biological function summary

IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.

Pathways

IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.

IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.
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Product protocols

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Target data

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed : 28811625, PubMed : 7699333). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.
See full target information Il7

Alternative Names

Il-7, Il7, Interleukin-7, IL-7
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Canadian journal of gastroenterology & hepatology 2019:9897315 PubMed30941331

2019

Copper Mediates Anti-Inflammatory and Antifibrotic Activity of Gleevec in Hepatocellular Carcinoma-Induced Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Iftekhar Hassan,Hossam Ebaid,Ibrahim M Alhazza,Jameel Al-Tamimi,Shazia Aman,Ahmad M Abdel-Mageed

Infection and immunity 84:108-19 PubMed26483405

2015

Treatment with Interleukin-7 Restores Host Defense against Pneumocystis in CD4+ T-Lymphocyte-Depleted Mice.

Applications

Unspecified application

Species

Unspecified reactive species

S Ruan,D R Samuelson,B Assouline,M Morre,J E Shellito
View all publications
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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