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AB227902

Mouse IL-7 ELISA Kit

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Mouse IL-7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-7 with a sensitivity of 12.046 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Il-7, Il7, Interleukin-7, IL-7

3 Images
Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)
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Supplier Data

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)

Mouse IL-7 Standard Curve Comparison Data.

Standard curve comparison between mouse IL-7 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)

Interpolated concentrations of spiked IL-7 in Mouse serum, plasma and cell culture supernatant samples.

The concentrations of IL-7 were measured in duplicates, interpolated from the IL-7 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50% and splenocyte supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-7 concentration was determined to be 2441.13 pg/mL in serum, 1535.45 pg/mL in plasma (heparin), and 2581.66g/mL in and splenocyte supernatant.

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-7 ELISA Kit (AB227902)

Example of Mouse IL-7 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 12.046 pg/mL

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL-7 ELISA Kit (ab227902) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-7 protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-7 with 12.046 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Unsuitable for EDTA plasma.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Spiked Media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Spiked Media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 105 %", "average": "= 107" }, { "sample": "Serum", "range": "83 - 88 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "81 - 83 %", "average": "= 83" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab227902-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB227902-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB227902-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-7 Lyophilized Recombinant Protein", "number":"AB227902-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227902-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-7 Capture Antibody", "number":"AB227902-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB227902-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB227902-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB227902-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-7 Detector Antibody", "number":"AB227902-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB227902-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
Biological function summary

IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.

Pathways

IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.

IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.
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Product protocols

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Target data

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed : 28811625, PubMed : 7699333). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.
See full target information Il7
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websiteProtocolBooklet
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