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AB277450

Mouse IL36 gamma/IL-1F9 ELISA Kit

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Mouse IL36 gamma/IL-1F9 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL36 gamma/IL-1F9 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Il1f9, Il36g, Interleukin-36 gamma, Interleukin-1 family member 9, IL-1F9

1 Images
ELISA - Mouse IL36 gamma/IL-1F9 ELISA Kit (AB277450)
  • ELISA

Supplier Data

ELISA - Mouse IL36 gamma/IL-1F9 ELISA Kit (AB277450)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.35 ng/mL

Range

0.328 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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AB197742

Mouse IL-1 beta ELISA Kit

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ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IL36 gamma/IL-1F9 ELISA Kit (ab277450) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL36 gamma/IL-1F9 in serum (Mouse gamma/IL-1F9 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse IL36 gamma/IL-1F9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse IL36 gamma/IL-1F9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse IL36 gamma/IL-1F9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse IL36 gamma/IL-1F9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "73 - 140 %", "average": "= 107.1" }, { "sample": "Plasma", "range": "86 - 148 %", "average": "= 115.3" }, { "sample": "Cell culture media", "range": "118 - 140 %", "average": "= 131.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277450-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277450-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277450-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277450-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse IL36 gamma IL-1F9 coated Microplate", "number":"AB277450-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse IL36 gamma/IL-1F9 Antibody", "number":"AB277450-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277450-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"450X HRP-Streptavidin Concentrate", "number":"AB277450-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL36 gamma/IL-1F9 standard protein (Lyophilized)", "number":"AB277450-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL36 gamma also known as IL-1F9 or IL-36 is a member of the interleukin-1 cytokine family. The protein weighs approximately 22 kDa. It is expressed highly in epithelial tissues such as keratinocytes in the skin bronchial tissues and intestinal lining. It acts mainly on nearby cells to influence immune responses through cytokine signaling.
Biological function summary

IL36 gamma plays a role in the immune and inflammatory responses. It operates as a cytokine that signals through the IL-1 receptor family leading to the activation of NF-kB and MAPK pathways. This member of the IL-1 family does not form a complex by itself but interacts with IL-36R to exert its functions. The protein influences processes such as cell proliferation differentiation and the regulation of immune responses to external pathogens or injuries.

Pathways

IL36 gamma is involved in inflammatory and immune signaling pathways. Within the immune response pathway it stimulates the production of other cytokines and pro-inflammatory molecules. IL36 gamma is associated with the IL-1 signaling pathway alongside other cytokines such as IL-1 and IL-18 which all contribute to immune modulation and inflammatory responses.

IL36 gamma has connections to psoriasis and inflammatory bowel disease. In psoriasis increased levels of IL36 gamma correlate with heightened skin inflammation and proliferation of keratinocytes. In inflammatory bowel disease IL36 gamma contributes to the inflammatory state of the gut. The activities of IL36 gamma often link with other cytokines such as TNF-alpha which collectively drive the inflammatory processes observed in these conditions.
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Product protocols

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Target data

Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in pro-inflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of pro-inflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes.
See full target information Il36g
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websiteProtocolBooklet
en
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Product promise

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