AB253219

Mouse iNOS ELISA Kit

Recombinant
SimpleStep
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(12 Publications)

Mouse iNOS ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse iNOS with a sensitivity of 18.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Inosl, Nos2, Inducible NO synthase, Macrophage NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Inducible NOS, iNOS, MAC-NOS

2 Images

Sandwich ELISA - Mouse iNOS ELISA Kit (AB253219)

sELISA

Unknown

Sandwich ELISA - Mouse iNOS ELISA Kit (AB253219)

Example of mouse iNOS standard curve in 1X Cell Extraction Buffer PTR.

The iNOS standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse iNOS ELISA Kit (AB253219)

Interpolated concentrations of native iNOS in RAW264.7 cell extract samples based on a 400 μg/mL extract load.

The concentrations of iNOS were measured in duplicate and interpolated from the iNOS standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean iNOS concentration was determined to be 1,052.8 pg/mL in RAW264.7 cell extract samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 18.1 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse iNOS ELISA Kit (ab253219) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of iNOS protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse iNOS with 18.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

iNOS, also known as NOS2, catalyzes the production of nitric oxide from L-arginine, which is used in various cell signaling events. iNOS is an inducible member of the NOS family and is upregulated as a host-defense mechanism during proinflammatory cytokine activity seen with tumorigenesis and parasitic infection. iNOS activity is a key cause of septic shock.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": null, "average": "= 110" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253219-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253219-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB253219-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse iNOS Lyophilized Recombinant Protein", "number":"AB253219-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253219-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253219-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse iNOS Capture Antibody", "number":"AB253219-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB253219-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253219-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB253219-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253219-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse iNOS Detector Antibody", "number":"AB253219-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB253219-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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Product protocols

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed : 16373578). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (By similarity).

See full target information Nos2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Iranian journal of public health 52:722-731 PubMed37551177

2023

Catalpol Alleviates Depression by Inhibiting NLRP3 Inflammasome via TLR4/MAPK/NF-Kb Pathway.

Applications

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Species

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Xuemei Liang,Yuhuan Zhao,Tianjiao Xu,Wei Wang,Weidong Sun,Rui Wang

Acta biochimica et biophysica Sinica 55:818-830 PubMed37232576

2023

The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

Applications

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Species

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Wenzhao Li,Cheng Tao,Minzhi Mao,Kewei Zhu

Polymers 14: PubMed36433125

2022

Preparation, Characterization, and Anti-Adhesive Activity of Sulfate Polysaccharide from against .

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Species

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Bao Le,Duy Thanh Do,Hien Minh Nguyen,Bich Hang Do,Huong Thuy Le

Discover. Oncology 13:119 PubMed36326938

2022

New thiophene derivative augments the antitumor activity of γ-irradiation against colorectal cancer in mice via anti-inflammatory and pro-apoptotic pathways.

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Species

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Nermeen M ElBakary,Sanaa A Hagag,Mohamed A Ismail,Wael M El-Sayed

Frontiers in immunology 13:1057746 PubMed36405726

2022

NLRP3+ macrophages aggravate inflammatory cystitis in diabetes.

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Species

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Yubing Peng,Yan Gao

Cell death & disease 13:863 PubMed36220838

2022

Neddylation-mediated degradation of hnRNPA2B1 contributes to hypertriglyceridemia pancreatitis.

Applications

Unspecified application

Species

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Wei Chen,Yilong Wang,Wenwen Xia,Jinbao Zhang,Yan Zhao

Disease markers 2022:9883831 PubMed36133436

2022

Activated Hepatic Stellate Cells Promote the M1 to M2 Macrophage Transformation and Liver Fibrosis by Elevating the Histone Acetylation Level.

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Species

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Junru Chen,Xueqing Huang,Zhi Huang,Yu Cao

Current issues in molecular biology 44:2967-2981 PubMed35877429

2022

A New EGFR Inhibitor from Exerted Potential Anti-Inflammatory Activity via Akt/PI3K Pathway Inhibition.

Applications

Unspecified application

Species

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Rania Alaaeldin,Heba Ali Hassan,Islam M Abdel-Rahman,Reham H Mohyeldin,Nancy Youssef,Ahmed E Allam,Sayed F Abdelwahab,Qing-Li Zhao,Moustafa Fathy

Journal of healthcare engineering 2021:3433615 PubMed34900182

2021

Astragaloside IV Reduces OxLDL-Induced BNP Overexpression by Regulating HDAC.

Applications

Unspecified application

Species

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Wenting Zhang,Xin Wang,Jing Li,Mingyuan Xu,Xiaolu Ren,Huiying Liu,Lu Xu,Jun Shao

Nature communications 11:6339 PubMed33311482

2020

Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Enyong Dai,Leng Han,Jiao Liu,Yangchun Xie,Herbert J Zeh,Rui Kang,Lulu Bai,Daolin Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse iNOS ELISA Kit

Sandwich ELISA - Mouse iNOS ELISA Kit (AB253219)

Sandwich ELISA - Mouse iNOS ELISA Kit (AB253219)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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