Mouse Insulin ELISA kit is a sandwich ELISA designed to quantify both native and recombinant mouse insulin (INS) with a sensitivity of 5 µIU/mL.
- Wide dynamic range – quantifies 6.25–400 µIU/mL
- Broad sample compatibility – works with serum, plasma (citrate, EDTA), and cell culture supernatants
- Assay platform – pre-coated microplate (12 x 8-well strips) for easy setup and reliable results
- Cited in over 15 publications
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Publications
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- Nature communications 14:84282023BACH1 controls hepatic insulin signaling and glucose homeostasis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayu Jin,Yunquan He,Jieyu Guo,Qi Pan,Xiangxiang Wei,Chen Xu,Zhiyuan Qi,Qinhan Li,Siyu Ma,Jiayi Lin,Nan Jiang,Jinghua Ma,Xinhong Wang,Lindi Jiang,Qiurong Ding,Elena Osto,Xiuling Zhi,Dan MengPubMed 38129407
- Journal of pharmacy and pharmacology research 7:192-2022023High Dose of Metformin Decreases Susceptibility to Occlusive Arterial Thrombosis in Diabetic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberto I Mota Alvidrez,Gowtham K Annarapu,Amudan J Srinivasan,Zeyu Liu,Hamza O Yazdani,Deidre Nolfi-Donegan,Richard L Simmons,Sruti Shiva,Matthew D NealPubMed 37975061
- Frontiers in pharmacology 14:11930062023Sulfosuccinimidyl oleate ameliorates the high-fat diet-induced obesity syndrome by reducing intestinal and hepatic absorption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiming Ma,Li Wen,Yanxia Tian,Liqin Ma,Zhangsheng Wen,Yang Kun,Mengping Xu,Xiaoping LiuPubMed 37305546
- JCI insight 8:2023l-Type amino acid transporter 1 in hypothalamic neurons in mice maintains energy and bone homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyujin Park,Kazuya Fukasawa,Tetsuhiro Horie,Yusuke Masuo,Yuka Inaba,Takanori Tatsuno,Takanori Yamada,Kazuya Tokumura,Sayuki Iwahashi,Takashi Iezaki,Katsuyuki Kaneda,Yukio Kato,Yasuhito Ishigaki,Michihiro Mieda,Tomohiro Tanaka,Kazuma Ogawa,Hiroki Ochi,Shingo Sato,Yun-Bo Shi,Hiroshi Inoue,Hojoon Lee,Eiichi HinoiPubMed 36862514
- Cell reports 42:1120782023Loss of C3a and C5a receptors promotes adipocyte browning and attenuates diet-induced obesity via activating inosine/A2aR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing-Ran Kong,Xiao-Hui Chen,Qing Sun,Kai-Yuan Zhang,Lian Xu,Liliqiang Ding,Yan-Ping Zhou,Ze-Bei Zhang,Jing-Rong Lin,Ping-Jin GaoPubMed 36735535
- Frontiers in endocrinology 13:10524872023Corticosterone induces obesity partly promoting intestinal cell proliferation and survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanhao Wang,Shuanqing Li,Yingqi Li,Meihui Zhang,Ting Xu,Tianming Li,Lining Cao,Jianfeng LuPubMed 36699046
- Nutrients 14:2022Anti-Obesity Effects of Multi-Strain Probiotics in Mice with High-Carbohydrate Diet-Induced Obesity and the Underlying Molecular Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye Rim Kim,Eunsol Seo,Seyeon Oh,MinYeong Seo,Kyunghee Byun,Byung-Yong KimPubMed 36501204
- Phytotherapy research : PTR 37:611-6262022Increasing brain glucose uptake by Gypenoside LXXV ameliorates cognitive deficits in a mouse model of diabetic Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangbao Meng,Yuan Zhang,Zongyang Li,Guoxu Ma,Xiejun Zhang,Di Zhang,Weiwei Cao,Sicen Wang,Qian Cai,Ping Cui,Guodong HuangPubMed 36325883
- Aging cell 21:e137262022High-fat diet-induced obesity augments the deleterious effects of estrogen deficiency on bone: Evidence from ovariectomized mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDalia Ali,Florence Figeac,Atenisa Caci,Nicholas Ditzel,Clarissa Schmal,Greet Kerckhofs,Jesper Havelund,Nils Faergeman,Alexander Rauch,Michaela Tencerova,Moustapha KassemPubMed 36217558
- Nature communications 13:59452022Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan XuPubMed 36209205
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 6.25 - 400 µlU/mL
- Sensitivity
- = 5 µlU/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Alternative names
Ins-1, Ins1, Insulin-1
What's included?
Recommended products
Mouse Insulin ELISA kit is a sandwich ELISA designed to quantify both native and recombinant mouse insulin (INS) with a sensitivity of 5 µIU/mL.
- Wide dynamic range – quantifies 6.25–400 µIU/mL
- Broad sample compatibility – works with serum, plasma (citrate, EDTA), and cell culture supernatants
- Assay platform – pre-coated microplate (12 x 8-well strips) for easy setup and reliable results
- Cited in over 15 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 6.25 - 400 µlU/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5 µlU/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111.2 | Range 90 - 132 % |
Sample type Cell culture media | Average % = 108.5 | Range 80 - 139 % |
Sample type Plasma | Average % = 89.67 | Range 70 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Mouse Insulin ELISA Kit ab277390 is a sandwich ELISA kit to measure mouse insulin (INS) with a sensitivity of 5 µIU/mL.
How the assay works
Mouse Insulin ELISA Kit ab277390 uses a pair of antibodies each specific to different epitopes at the target of interest: the capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Mouse Insulin ELISA Kit ab277390 protocol summary
- Add standards or samples to the wells, incubate 2 hrs
- Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
- Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
- Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
- Add 50 µl of Stop Solution per well. Read at 450 nm immediately
How other researchers are using Mouse Insulin ELISA Kit ab277390
Mouse Insulin ELISA Kit ab277390 has been used in publications to study the mechanisms of sulfosuccinimidyl oleate in fatty acid transport as a potential treatment for obesity (1) and the mechanisms of hypothalamic neuron regulation on bone homeostasis(2).
References:
(1)Xiaoping Liu et al. 20223 PMID: 37305546,(2)Eiichi Hinoi et al. 2023 PMID: 36862514
Related and recommended products
Mouse Insulin ELISA Kit ab277390 is commonly used to study diabetes and insulin disorders.
Other kits for measuring metabolic health include:
- Mouse Albumin ELISA Kit Mouse Albumin ELISA Kit ab207620
- Mouse Leptin ELISA Kit Mouse Leptin ELISA Kit ab199082
- Mouse Adiponectin ELISA Kit Mouse Adiponectin ELISA Kit ab226900
Mouse Insulin ELISA Kit ab277390 is a sandwich ELISA to measure mouse Insulin (mouse ins) in serum, plasma, and cell culture supernatant with a sensitivity of 5 µIU/mL.
How the assay works
This assay employs an antibody specific for Mouse Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay specificity
This assay detects mouse, human, rat, porcine and bovine Insulin.
Mouse Insulin ELISA Kit ab277390 protocol summary
1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add biotinylated antibody. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
How other researchers are using Mouse Insulin ELISA Kit ab277390
Mouse Insulin ELISA Kit ab277390 has been used in publications to study the mechanisms of sulfosuccinimidyl oleate in fatty acid transport as a potential treatment for obesity (1) and the mechanisms of hypothalamic neuron regulation on bone homeostasis(2).
References:
(1)Xiaoping Liu et al. 20223 PMID: 37305546,
(2)Eiichi Hinoi et al. 2023 PMID: 36862514
Related and recommended products
Mouse Insulin ELISA Kit ab277390 is commonly used to study diabetes and insulin disorders.
Other kits for measuring metabolic health include:
- Mouse Albumin ELISA Kit Mouse Albumin ELISA Kit ab207620
- Mouse Leptin ELISA Kit Mouse Leptin ELISA Kit ab199082
- Mouse Adiponectin ELISA Kit Mouse Adiponectin ELISA Kit ab226900
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
- Biological function summary
Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.
- Pathways
Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.
- Associated diseases and disorders
Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
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ELISA - Mouse Insulin ELISA Kit (ab277390)
These standard curves are for demonstration only. A standard curve must be run with each assay.
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