JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277390

Mouse Insulin ELISA Kit

Be the first to review this product! Submit a review

|

(33 Publications)

Mouse Insulin ELISA kit is a sandwich ELISA designed to quantify both native and recombinant mouse insulin (INS) with a sensitivity of 5 µIU/mL.

- Wide dynamic range – quantifies 6.25–400 µIU/mL
- Broad sample compatibility – works with serum, plasma (citrate, EDTA), and cell culture supernatants
- Assay platform – pre-coated microplate (12 x 8-well strips) for easy setup and reliable results
- Cited in over 15 publications

View Alternative Names

Ins-1, Ins1, Insulin-1

1 Images
ELISA - Mouse Insulin ELISA Kit (AB277390)
  • ELISA

Supplier Data

ELISA - Mouse Insulin ELISA Kit (AB277390)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5 µlU/mL

Range

6.25 - 400 µlU/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB199082

Mouse Leptin ELISA Kit

SimpleStep

ELISA - Mouse Leptin ELISA Kit (AB199082)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB226900

Mouse Adiponectin ELISA Kit

SimpleStep

ELISA - Mouse Adiponectin ELISA Kit (AB226900)

  • 0
  • 0
Assay Kits

AB65336

Triglyceride Assay Kit - Quantification

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

  • 4
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse Insulin ELISA Kit ab277390 is a sandwich ELISA to measure mouse Insulin (mouse ins) in serum, plasma, and cell culture supernatant with a sensitivity of 5 µIU/mL.

How the assay works

This assay employs an antibody specific for Mouse Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay specificity

This assay detects mouse, human, rat, porcine and bovine Insulin.

Mouse Insulin ELISA Kit ab277390 protocol summary

1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add biotinylated antibody. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.

How other researchers are using Mouse Insulin ELISA Kit ab277390

Mouse Insulin ELISA Kit ab277390 has been used in publications to study the mechanisms of sulfosuccinimidyl oleate in fatty acid transport as a potential treatment for obesity(1) and the mechanisms of hypothalamic neuron regulation on bone homeostasis(2).

References:

(1) Xiaoping Liu et al. 20223 PMID: 37305546,
(2) Eiichi Hinoi et al. 2023 PMID: 36862514

Related and recommended products

Mouse Insulin ELISA Kit ab277390 is commonly used to study diabetes and insulin disorders.

Other kits for measuring metabolic health include:

- Mouse Albumin ELISA Kit ab207620
- Mouse Leptin ELISA Kit ab199082
- Mouse Adiponectin ELISA Kit ab226900

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "90 - 132 %", "average": "= 111.2" }, { "sample": "Cell culture media", "range": "80 - 139 %", "average": "= 108.5" }, { "sample": "Plasma", "range": "70 - 109 %", "average": "= 89.67" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277390-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277390-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277390-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse Insulin coated Microplate", "number":"AB277390-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB277390-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse Insulin Antibody", "number":"AB277390-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277390-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB277390-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Insulin standard protein (Lyophilized)", "number":"AB277390-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277390-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
style
left-column

Product protocols

style
left-column

Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information Ins1
style
left-column

Publications (33)

Recent publications for all applications. Explore the full list and refine your search

World journal of diabetes 16:103685 PubMed40548264

2025

Erianin mitigates diabetic cardiomyopathy adenosine monophosphate-activated protein kinase-nuclear factor erythroid 2-related factor 2-heme oxygenase-1 pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Hui Chen,Xiao-Chun Dai,Zi-Jiao Quan,Xin-Yu Liu

Diabetology & metabolic syndrome 17:193 PubMed40474280

2025

IL-37 inhibited inflammation to improve gestational diabetes mellitus through the GSK3/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meiyu Song,Guanli Zhang

Experimental biology and medicine (Maywood, N.J.) 250:10356 PubMed40458821

2025

Chronic administration of a cannabis-derived mixture at an antihyperalgesic dose does not significantly enhance hepatotoxicity or the development of metabolic dysfunction-associated steatohepatitis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kim B Pedersen,Tomislav Jelesijevic,Tamara M Morris,Sarah M Melton,Ashley S Henderson,John F Glenn,Gregory J Davenport,Martin J J Ronis,Peter J Winsauer

Journal of cellular and molecular medicine 29:e70352 PubMed39855896

2025

Stromal Cell Derived Factor-1 Promotes Hepatic Insulin Resistance via Inhibiting Hepatocyte Lipophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfeng Lu,Yuting Zhang,Cuilian Sun,Yuhang Na,Haotian Sun,Jianhua Ma,Xueqin Wang,Xiaomin Cang

Biomedicines 13: PubMed39857767

2025

Olivetol's Effects on Metabolic State and Gut Microbiota Functionality in Mouse Models of Alimentary Obesity, Diabetes Mellitus Type 1 and 2, and Hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia A Zabolotneva,Katerina E Popruga,Valentin V Makarov,Sergei M Yudin,Andrei M Gaponov,Sergei A Roumiantsev,Aleksandr V Shestopalov

BMJ open diabetes research & care 13: PubMed39842865

2025

Mechanism of TGIF1 on glycolipid metabolism disorders in mice with type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Fuyan Bai,Liping Zheng,Li Tao,Shikai Wang,Yuchen Li,Lijun Hou

Scientific reports 15:1487 PubMed39789226

2025

Exercise promotes peripheral glycolysis in skeletal muscle through miR-204 induction via the HIF-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sang R Lee,Kang Joo Jeong,Moeka Mukae,Jinhee Lee,Eui-Ju Hong

Frontiers in pharmacology 15:1484278 PubMed39654627

2024

Osteopontin neutralization increases vitamin D receptors on NKT cells and ameliorates liver fibrosis by promoting their activity.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Enas Hussini,Rasha Shweiki,Iman Zahran,Mohammad Far

Theranostics 14:5596-5607 PubMed39310111

2024

pH and HO dual-sensitive nanoparticles enable enhanced and safe glucose-responsive oral insulin delivery for diabetes mellitus treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Muzi Li,Nan Wang,Ruiyuan Liu,Xinyue Zhang,Wei He,Wen Zhang,Jiaxin Li,Chen Peng,Yan Li

Frontiers in pharmacology 15:1412453 PubMed39108759

2024

Platycodon D reduces obesity and non-alcoholic fatty liver disease induced by a high-fat diet through inhibiting intestinal fat absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Xingkui Tang,Yi Yang,Wenxu Peng,Mengping Xu,Qitong Fan,Feng Li,Guorong Zou,Jianlin Zhu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com