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AB285341

Mouse Insulin ELISA Kit

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(3 Publications)

Mouse Insulin ELISA Kit is a sandwich ELISA designed to quantify Mouse Insulin with a sensitivity of 46.9 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78 - 5000 pg/mL

View Alternative Names

Ins-1, Ins1, Insulin-1

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Sandwich ELISA - Mouse Insulin ELISA Kit (AB285341)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Insulin ELISA Kit (AB285341)

Mouse Insulin ELISA Kit - Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 46.9 pg/mL

Range

78 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Mouse Insulin ELISA Kit ab285341 is a sandwich ELISA to measure Mouse Insulin in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, other biological fluids with a sensitivity of 46.9 pg/ml.

How the assay works

The density of color is proportional to the amount of human Insulin captured from the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Insulin ELISA Kit ab285341 protocol summary

1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called K4271 Insulin (Mouse) ELISA Kit. K4271-100 is the same size as the 96 test size of ab285341.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285341-96Test", "assetComponentDetails": [ { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285341-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285341-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285341-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285341-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285341-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285341-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285341-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285341-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285341-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285341-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotin-Detection antibody (Lyophilized)", "number":"AB285341-CMP12", "productcode":"" }, { "size":"1 x 200 µL", "name":"Purified water", "number":"AB285341-CMP13", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information Ins1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6384 PubMed37821436

2023

Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activation.

Applications

Unspecified application

Species

Unspecified reactive species

Minxuan Xu,Jun Tan,Xin Liu,Li Han,Chenxu Ge,Yujie Zhang,Fufang Luo,Zhongqin Wang,Xiaoqin Xue,Liangyin Xiong,Xin Wang,Qinqin Zhang,Xiaoxin Wang,Qin Tian,Shuguang Zhang,Qingkun Meng,Xianling Dai,Qin Kuang,Qiang Li,Deshuai Lou,Linfeng Hu,Xi Liu,Gang Kuang,Jing Luo,Chunxiao Chang,Bochu Wang,Jie Chai,Shengbin Shi,Lianyi Han

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302130 PubMed37544908

2023

Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S-Palmitoylated IRHOM2.

Applications

Unspecified application

Species

Unspecified reactive species

Minxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Yi Zhang,Fufeng Gao,Xianling Dai,Qin Kuang,Jie Chai,Benkui Zou,Bochu Wang

Acta pharmaceutica Sinica. B 13:1071-1092 PubMed36970206

2023

The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis blocking inactive rhomboid protein 2-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Minxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Wei Dong,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Qiang Li,Deshuai Lou,Linfeng Hu,Xi Liu,Gang Kuang,Jing Luo,Jing Feng,Bochu Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com