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Mouse Interferon alpha 1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Interferon alpha 1 in Cell culture media, Heparin Plasma, Citrate plasma, Serum samples.
Alternative names=Interferon alpha-1, IFN-alpha-1, Ifna1
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Citrate plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
15.6 - 1000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
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Mouse Interferon alpha 1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Interferon alpha 1 in Cell culture media, Heparin Plasma, Citrate plasma, Serum samples.
Alternative names=Interferon alpha-1, IFN-alpha-1, Ifna1
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Citrate plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
15.6 - 1000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 89 | Range 82 - 95 % |
Sample type Cell culture media | Average % = 100 | Range 95 - 106 % |
Sample type Heparin Plasma | Average % = 89 | Range 88 - 91 % |
Sample type Citrate plasma | Average % = 89 | Range 85 - 92 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse Interferon alpha ELISA Kit (ab252352) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN-alpha protein in cell culture media, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IFN-alpha with 7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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