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AB252352

Mouse Interferon alpha 1 ELISA Kit

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(13 Publications)

Mouse Interferon alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Interferon alpha 1 with a sensitivity of 7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Interferon alpha-1, IFN-alpha-1, Ifna1

4 Images
ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)
  • ELISA

Supplier Data

ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)
Sandwich ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)
  • sELISA

Lab

Sandwich ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)

Example of mouse Interferon-alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)
  • sELISA

Unknown

Sandwich ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)

Interpolated concentrations of spike IFN-alpha in mouse serum, plasma and cell culture media samples.

The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and RPMI cell culture media containing 10% fetal bovine serum 100%.

Sandwich ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)
  • sELISA

Unknown

Sandwich ELISA - Mouse Interferon alpha 1 ELISA Kit (AB252352)

Example of mouse IFN-alpha standard curve in Sample Diluent NS.

The IFN-alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 7 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Interferon alpha 1 ELISA Kit ab252352 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Interferon alpha 1 in cell culture media, citrate plasma, heparin plasma, serum. This SimpleStep sensitivity is 7 pg/mL.

How the assay works

Mouse Interferon alpha 1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Interferon alpha 1 ELISA Kit ab252352 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse Interferon alpha 1 ELISA Kit only requires a maximum of 12.5µL sample
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 95 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "95 - 106 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "88 - 91 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "85 - 92 %", "average": "= 89" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.
Biological function summary

Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.

Pathways

Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.

Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.
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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information Ifna1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Oncology 32:200898 PubMed39640863

2024

Newcastle disease virus harboring the PTEN gene inhibits pancreatic cancer growth by inhibiting PI3K/AKT/mTOR signaling and activating apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Seonhee Kim,Bo-Kyoung Jung,Jinju Kim,Joo Hee Jeon,Sung Hoon Jang,Minsoo Kim,Cuk-Seong Kim,Hyun Jang

Immunity 57:1696-1709.e10 PubMed38878770

2024

The brain microvasculature is a primary mediator of interferon-α neurotoxicity in human cerebral interferonopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Barney Viengkhou,Emina Hayashida,Sarah McGlasson,Katie Emelianova,Deborah Forbes,Stewart Wiseman,Joanna Wardlaw,Rovin Verdillo,Sarosh R Irani,Darragh Duffy,Fredrik Piehl,Lipin Loo,Axel Pagenstecher,G Greg Neely,Yanick J Crow,Iain L Campbell,David P J Hunt,Markus J Hofer

Cell & bioscience 13:220 PubMed38037116

2023

GSTM2 alleviates heart failure by inhibiting DNA damage in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfei Xu,Zhen Wang,Yalin Wang,Shaobo Pan,Wenting Zhao,Miao Chen,Xiaofan Chen,Tingting Tao,Liang Ma,Yiming Ni,Weidong Li

Nature communications 14:1606 PubMed36959204

2023

Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotu Ma,Xiaolong Liang,Yao Li,Qingqing Feng,Keman Cheng,Nana Ma,Fei Zhu,Xinjing Guo,Yale Yue,Guangna Liu,Tianjiao Zhang,Jie Liang,Lei Ren,Xiao Zhao,Guangjun Nie

EMBO reports 24:e55286 PubMed36652307

2023

IFN-stimulated metabolite transporter ENT3 facilitates viral genome release.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ting Hsieh,Tsung-Lin Tsai,Shen-Yan Huang,Jian-Wen Heng,Yu-Chia Huang,Pei-Yuan Tsai,Chia-Chun Tu,Tai-Ling Chao,Ya-Min Tsai,Pei-Ching Chang,Chien-Kuo Lee,Guann-Yi Yu,Sui-Yuan Chang,Ivan L Dzhagalov,Chia-Lin Hsu

Annals of the New York Academy of Sciences 1518:299-314 PubMed36303271

2022

Epidermal growth factor activates a hypoxia-inducible factor 1α-microRNA-21 axis to inhibit aquaporin 4 in chronic rhinosinusitis.

Applications

Unspecified application

Species

Unspecified reactive species

Junjun Chen,Dong Liu,Jingpu Yang,Chengxun Jin,Chang Zhao,Jinzhang Cheng

mBio 13:e0257822 PubMed36222510

2022

Macrophage Mitochondrial Biogenesis and Metabolic Reprogramming Induced by Leishmania donovani Require Lipophosphoglycan and Type I Interferon Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hamlet Acevedo Ospina,Marie-Michèle Guay-Vincent,Albert Descoteaux

Cancer cell 40:1145-1160.e9 PubMed36150390

2022

Ablation of the endoplasmic reticulum stress kinase PERK induces paraptosis and type I interferon to promote anti-tumor T cell responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica K Mandula,Shiun Chang,Eslam Mohamed,Rachel Jimenez,Rosa A Sierra-Mondragon,Darwin C Chang,Alyssa N Obermayer,Carlos M Moran-Segura,Satyajit Das,Julio A Vazquez-Martinez,Karol Prieto,Ann Chen,Keiran S M Smalley,Brian Czerniecki,Peter Forsyth,Richard C Koya,Brian Ruffell,Juan R Cubillos-Ruiz,David H Munn,Timothy I Shaw,Jose R Conejo-Garcia,Paulo C Rodriguez

Frontiers in immunology 12:758190 PubMed34867997

2021

Mutation in Gene ( ) Impairs Type I IFN-Mediated Antiviral Immune Response by Murine pDCs.

Applications

Unspecified application

Species

Unspecified reactive species

Annesa Das,Kuldeep Singh Chauhan,Himanshu Kumar,Prafullakumar Tailor

Journal of cellular and molecular medicine 25:9939-9952 PubMed34581005

2021

Interleukin-7 aggravates myocardial ischaemia/reperfusion injury by regulating macrophage infiltration and polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Mengwen Yan,Yaliu Yang,Ying Zhou,Changan Yu,Rui Li,Wei Gong,Jingang Zheng
View all publications
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