Mouse Interferon alpha 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Interferon alpha 2 with a sensitivity of 0.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Neuron 107:891-908.e82020Cell Type-Specific Transcriptomics Reveals that Mutant Huntingtin Leads to Mitochondrial RNA Release and Neuronal Innate Immune Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyeseung Lee et. al.PubMed 32681824
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.36 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Produced by macrophages, IFN-alpha have antiviral activities.
Alternative names
Interferon alpha-2, IFN-alpha-2, Ifna2
What's included?
Recommended products
Mouse Interferon alpha 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Interferon alpha 2 with a sensitivity of 0.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.36 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.9 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 2.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 2.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97 | Range 94 - 100 % |
Sample type Serum | Average % = 94 | Range 93 - 97 % |
Sample type Cell culture extracts | Average % = 96 | Range 92 - 101 % |
Sample type Citrate plasma | Average % = 85 | Range 85 - 86 % |
Sample type EDTA Plasma | Average % = 96 | Range 95 - 98 % |
Sample type Heparin Plasma | Average % = 96 | Range 93 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Interferon alpha 2 ELISA Kit (ab211648) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon alpha 2 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Interferon alpha 2 with 0.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
There are three major structural types of interferons: alpha, beta, and gamma. Interferon alpha forms are produced by monocytes/macrophages, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, and glucocorticoid hormones. Interferon alpha-2 is one of at least 23 different known variants of interferon alpha. The individual interferon alpha proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156 - 172 amino acids (aa). Mouse Interferon alpha-2 is 167 aa in length. Interferon alpha-2 demonstrates antiviral, antiparasitic, and antiproliferative activities.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon alpha 2 also known as IFN alpha 2 or IFN alpha 2a/b is a type of cytokine that plays a critical role in the immune response. It is a smaller protein weighing approximately 19.5 kDa mainly expressed by leukocytes such as macrophages and dendritic cells. This protein exhibits antiviral antiproliferative and immunomodulatory activities by binding to specific receptors on target cells. Researchers often study interferon alpha through recombinant protein studies which can be simulated by companies like PeproTech. Additionally ELISA assays facilitate the quantitative measurement of interferon alpha levels in various biological samples.
- Biological function summary
Interferon alpha 2 engages in complex signaling with other immune factors to establish an antiviral state in cells. This protein binds to the IFNAR1 and IFNAR2 receptor complex initiating a cascade that activates genes responsible for immune defense. By doing so it triggers the transcription of various antiviral proteins and enhances the activity of immune cells including natural killer cells and macrophages. Its function is also evaluated in reconstitution studies such as PeproTech's IL-2 reconstitution to investigate broader immune functions.
- Pathways
Researchers observe interferon alpha 2 predominantly within the JAK-STAT signaling pathway. This pathway is vital in transmitting the extracellular signal through to the gene transcription process leading to an appropriate immune response. STAT proteins such as STAT1 and STAT2 form a transcription complex following activation by JAK kinases. Interferon alpha 2's interactions in the pathway exemplify how this cytokine links with other components for immune regulation coordinating with other proteins like ISGF3 to amplify antiviral responses.
- Associated diseases and disorders
Scientists associate interferon alpha 2 with chronic viral infections and certain cancers. For example its therapeutic application has been prominent in treating hepatitis C and some hematological malignancies. In viral infections interferon alpha inhibits viral replication and enhances antiviral immunity. In cancer it suppresses cell proliferation and regulates immune surveillance. The protein's levels and activity can correlate with pathological states influenced by other proteins such as IFN-lambda and TNF-alpha highlighting its role in disease mechanisms and therapeutic interventions.
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6 product images
Sandwich ELISA - Mouse Interferon alpha 2 ELISA Kit (ab211648)
Example of mouse Interferon alpha-2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Interferon alpha 2 ELISA Kit (ab211648)
Example of mouse Interferon alpha-2 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Interferon alpha 2 ELISA Kit (ab211648)
Interpolated concentrations of spiked Interferon alpha-2 in mouse serum and plasma samples.
The concentrations of Interferon alpha-2 were measured in duplicates, interpolated from the Interferon alpha-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 196.9 pg/mL in serum, 181.9 pg/mL in plasma (citrate), 204.1 pg/mL in plasma (EDTA), and 194.9 pg/mL in plasma (heparin).
Sandwich ELISA - Mouse Interferon alpha 2 ELISA Kit (ab211648)
Interpolated concentrations of spiked Interferon alpha-2 in mouse cell culture supernatant samples.
The concentrations of Interferon alpha-2 were measured in duplicates, interpolated from the Interferon alpha-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: 100% RAW 264.7 stimulated supernatant, 100% J774A.1 stimulated supernatant, and 100% PBMC stimulated supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 466.4 pg/mL in spiked RAW 264.7 stimulated supernatant, 500.1 pg/mL in spiked J774A.1 stimulated supernatant, and 518.9 pg/mL in spiked PBMC stimulated supernatant. RAW 264.7 supernatant was stimulated for 48 hours with 5 μg/mL of LPS. J774A.1 supernatant was stimulated for 72 hours with 1.5% PHA and 10 ng/mL PMA. PBMC supernatant was stimulated for 5 hours with 3 μg/mL of imiquimod.
Sandwich ELISA - Mouse Interferon alpha 2 ELISA Kit (ab211648)
Interpolated concentrations of spiked Interferon alpha-2 in mouse J774A.1 and PBMC extracts based on a 50 μg/mL extract load.
The concentrations of Interferon alpha-2 were measured in duplicate and interpolated from the Interferon alpha-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 435.6 pg/mL in spiked J774A.1 extract and 439.4 pg/mL in spiked PBMC extract.
Sandwich ELISA - Mouse Interferon alpha 2 ELISA Kit (ab211648)
Interpolated concentrations of native Interferon alpha-2 in mouse J774A.1 extract based on a 50 μg/mL extract load.
The concentrations of Interferon alpha-2 were measured in duplicate and interpolated from the Interferon alpha-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 57.4 pg/mL in J774A.1 extract.
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