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AB46081

Mouse Interferon gamma ELISA Kit

5

(1 Review)

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(7 Publications)

Mouse Interferon gamma ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Interferon gamma in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

Interferon gamma, IFN-gamma, Ifng

1 Images
Sandwich ELISA - Mouse Interferon gamma ELISA Kit (AB46081)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Interferon gamma ELISA Kit (AB46081)

Representative Standard Curve using ab46081

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse interferon gamma ELISA kit (IFN Gamma) is designed for the quantitative measurement of IFN gamma in mouse serum, buffered solutions and cell culture medium.

A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

This kit will recognize both endogenous and recombinant mouse IFN gamma.

Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 9, "mean": "128.8 pg/mL", "standardDeviation": "7.4", "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 10, "mean": "546.9 pg/mL", "standardDeviation": "25.2", "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 19, "mean": "588.6 pg/mL", "standardDeviation": "19.1", "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 20, "mean": "315.4 pg/mL", "standardDeviation": "14.5", "coefficientOfVariability": "4.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 11585387, PubMed : 8456301). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (PubMed : 20535209, PubMed : 25078851).
See full target information Ifng
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadt6113 PubMed40249812

2025

Progesterone signaling in oviductal epithelial cells modulates the immune response to support preimplantation embryonic development.

Applications

Unspecified application

Species

Unspecified reactive species

Emily A McGlade,Jiude Mao,Kalli K Stephens,Ryan M Marquardt,Feyza Nur Arguc,Peter F Lais,San-Pin Wu,Sarayut Winuthayanon,Haval Shirwan,Esma S Yolcu,Mark I Hunter,James K Pru,John P Lydon,Francesco J DeMayo,Wipawee Winuthayanon

International journal of molecular sciences 24: PubMed36834581

2023

Study of Effector CD8+ T Cell Interactions with Cortical Neurons in Response to Inflammation in Mouse Brain Slices and Neuronal Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Hsin Lin,Anja Scheller,Yang Liu,Elmar Krause,Hsin-Fang Chang

Journal for immunotherapy of cancer 10: PubMed35039461

2022

Combination of ultrasound-based mechanical disruption of tumor with immune checkpoint blockade modifies tumor microenvironment and augments systemic antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Abe,Hiroshi Nagata,Erika J Crosby,Yoshiyuki Inoue,Kensuke Kaneko,Cong-Xiao Liu,Xiao Yang,Tao Wang,Chaitanya R Acharya,Pankaj Agarwal,Joshua Snyder,William Gwin,Michael A Morse,Pei Zhong,Herbert Kim Lyerly,Takuya Osada

Scientific reports 11:256 PubMed33420261

2021

Gamma-radiated immunosuppressed tumor xenograft mice can be a new ideal model in cancer research.

Applications

Unspecified application

Species

Unspecified reactive species

Hamid Khodayari,Saeed Khodayari,Solmaz Khalighfard,Arash Tahmasebifar,Mahboubeh Tajaldini,Amirhoushang Poorkhani,Hassan Nikoueinejad,Gholam Ali Hamidi,Hassan Nosrati,Mohammad Reza Kalhori,Ali Mohammad Alizadeh

EMBO molecular medicine 12:e11210 PubMed32885605

2020

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Alessio Menga,Marina Serra,Simona Todisco,Carla Riera-Domingo,Ummi Ammarah,Manuel Ehling,Erika M Palmieri,Maria Antonietta Di Noia,Rosanna Gissi,Maria Favia,Ciro L Pierri,Paolo E Porporato,Alessandra Castegna,Massimiliano Mazzone

Nature metabolism 1:276-290 PubMed31489403

2019

MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Sousa-Victor,Joana Neves,Wendy Cedron-Craft,P Britten Ventura,Chen-Yu Liao,Rebeccah R Riley,Ilya Soifer,Nicholas van Bruggen,Ganesh A Kolumam,Saul A Villeda,Deepak A Lamba,Heinrich Jasper

Drug design, development and therapy 9:217-39 PubMed25565775

2015

Synthesis and characterization of a novel chemically designed (Globo)3-DTPA-KLH antigen.

Applications

sELISA

Species

Mouse

Mehdi Hajmohammadi,Seyed Davar Siadat,Masoud Ghorbani,Mehdi Shafiee Ardestani,Shahram Teimourian,Vahid Asgari,Reza Ahangari Cohan,Mostafa Hajmohammadi,Akram Hajmohammadi,Ramezan Behzadi,Saied Rajab Nezhad,Nabiollah Namvar Asl
View all publications
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