Mouse Interferon gamma ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Interferon gamma in Mouse in Cell culture supernatant, Serum samples.
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- International journal of molecular sciences 24:2023Study of Effector CD8+ T Cell Interactions with Cortical Neurons in Response to Inflammation in Mouse Brain Slices and Neuronal Cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Hsin Lin,Anja Scheller,Yang Liu,Elmar Krause,Hsin-Fang ChangPubMed 36834581
- Journal for immunotherapy of cancer 10:2022Combination of ultrasound-based mechanical disruption of tumor with immune checkpoint blockade modifies tumor microenvironment and augments systemic antitumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShinya Abe,Hiroshi Nagata,Erika J Crosby,Yoshiyuki Inoue,Kensuke Kaneko,Cong-Xiao Liu,Xiao Yang,Tao Wang,Chaitanya R Acharya,Pankaj Agarwal,Joshua Snyder,William Gwin,Michael A Morse,Pei Zhong,Herbert Kim Lyerly,Takuya OsadaPubMed 35039461
- Scientific reports 11:2562021Gamma-radiated immunosuppressed tumor xenograft mice can be a new ideal model in cancer research.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHamid Khodayari,Saeed Khodayari,Solmaz Khalighfard,Arash Tahmasebifar,Mahboubeh Tajaldini,Amirhoushang Poorkhani,Hassan Nikoueinejad,Gholam Ali Hamidi,Hassan Nosrati,Mohammad Reza Kalhori,Ali Mohammad AlizadehPubMed 33420261
- EMBO molecular medicine 12:e112102020Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessio Menga et. al.PubMed 32885605
- Nature metabolism 1:276-2902019MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPedro Sousa-Victor et. al.PubMed 31489403
- Drug design, development and therapy 9:217-392015Synthesis and characterization of a novel chemically designed (Globo)3-DTPA-KLH antigen.Applications:sELISAReactive species:MouseMehdi Hajmohammadi,Seyed Davar Siadat,Masoud Ghorbani,Mehdi Shafiee Ardestani,Shahram Teimourian,Vahid Asgari,Reza Ahangari Cohan,Mostafa Hajmohammadi,Akram Hajmohammadi,Ramezan Behzadi,Saied Rajab Nezhad,Nabiollah Namvar AslPubMed 25565775
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 1000 pg/mL
- Assay time
- 3h 45m
- Sensitivity
- < 15 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:11585387, PubMed:8456301). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (PubMed:20535209, PubMed:25078851).
Alternative names
Interferon gamma, IFN-gamma, Ifng
What's included?
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Mouse Interferon gamma ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Interferon gamma in Mouse in Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 1000 pg/mL
- Assay time
- 3h 45m
- Assay Platform
- Microplate
- Sensitivity
- < 15 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample B | n 19 | mean 588.6 pg/mL | SD 19.1 | C.V. 4.6 |
Sample Sample A | n 20 | mean 315.4 pg/mL | SD 14.5 | C.V. 4.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 9 | mean 128.8 pg/mL | SD 7.4 | C.V. 5.7 |
Sample Sample B | n 10 | mean 546.9 pg/mL | SD 25.2 | C.V. 4.6 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse interferon gamma ELISA kit (IFN Gamma) is designed for the quantitative measurement of IFN gamma in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant mouse IFN gamma.
Get results in 90 minutes with Human IFN gamma ELISA Kit (Human IFN gamma ELISA Kit ab174443) from our SimpleStep ELISA® range.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
- Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.
- Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
- Associated diseases and disorders
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Sandwich ELISA - Mouse Interferon gamma ELISA Kit (ab46081)
Representative Standard Curve using ab46081
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