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Mouse Interferon gamma ELISA Kit (IFNG) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Interferon gamma (IFNG) in Mouse in Cell culture extracts, Tissue Extracts samples.
Alternative names=Interferon gamma, IFN-gamma, Ifng
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
1.3 - 2000 pg/mL
- Sensitivity
= 1.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse Interferon gamma ELISA Kit (IFNG) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Interferon gamma (IFNG) in Mouse in Cell culture extracts, Tissue Extracts samples.
Alternative names=Interferon gamma, IFN-gamma, Ifng
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
1.3 - 2000 pg/mL
- Assay Platform
Microplate
- Sensitivity
= 1.3 pg/mL
- General recovery
92%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 91.95 | Range 84 - 104 % |
Sample type Tissue Extracts | Average % = 93.47 | Range 83 - 103 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Interferon gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Interferon gamma in cell lysate and tissue lysate.
This assay employs an antibody specific for Mouse Interferon gamma coated on a 96- well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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