AB260067

Mouse IP-10 ELISA Kit (CXCL10)

SimpleStep
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(5 Publications)

Mouse IP-10 ELISA Kit (CXCL10) is a single-wash 90-min Simplestep used to quantify Mouse IP-10 (CXCL10) with a sensitivity of 21.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Cited in over 5 citations

View Alternative Names

Crg2, Ifi10, Inp10, Scyb10, Cxcl10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, C7, Interferon-gamma induced protein CRG-2, Small-inducible cytokine B10, Gamma-IP10, IP-10

8 Images

ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

ELISA

Supplier Data

ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

sELISA

Supplier Data

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IP-10 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Interpolated concentration of native IP-10 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.75% stimulated RAW264.7 supernatant (1 µg/mL LPS for 48 hrs). The interpolated dilution factor corrected (to neat) values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Example of mouse IP-10 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Linearity of dilution.

Recombinant IP-10 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Neat pooled serum and plasma (citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

sELISA

Supplier Data

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Example of mouse IP-10 standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Interpolated concentrations of native IP-10 in mouse RAW264.7 cell culture supernatant samples stimulated with 1 µg/mL for 18 hours.

The concentrations of IP-10 were measured in duplicates, interpolated from the IP-10 standard curves and corrected for sample dilution. Undiluted samples are as follows : RAW264.7 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IP-10 concentration was determined to be 442.08 pg/mL in RAW264.7 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Example of mouse IP-10 standard curve in Sample Diluent NS.

The IP-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 21.75 pg/mL

Range

64 - 4100 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IP-10 ELISA Kit (CXCL10) ab260067 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IP-10 (CXCL10)in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 21.75 pg/mL.

How the assay works

Mouse IP-10 (CXCL10)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IP-10 ELISA Kit (CXCL10) ab260067 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse IP-10 ELISA Kit (CXCL10) only requires a maximum of 12.5µL sample

C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 115 %", "average": "= 104" }, { "sample": "Serum", "range": "92 - 102 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "84 - 99 %", "average": "= 89" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.

Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.

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Product protocols

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Target data

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity) (PubMed : 28623423). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (PubMed : 18624292, PubMed : 19017990, PubMed : 28468883). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (PubMed : 15456824).

See full target information Cxcl10

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7709 PubMed38001101

2023

Low-dose radiotherapy combined with dual PD-L1 and VEGFA blockade elicits antitumor response in hepatocellular carcinoma mediated by activated intratumoral CD8 exhausted-like T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Li,Kun Li,Kang Wang,Haoyuan Yu,Xiangyang Wang,Mengchen Shi,Zhixing Liang,Zhou Yang,Yongwei Hu,Yang Li,Wei Liu,Hua Li,Shuqun Cheng,Linsen Ye,Yang Yang

Neurobiology of disease 176:105964 PubMed36526090

2022

TNF and IL6/Jak2 signaling pathways are the main contributors of the glia-derived neuroinflammation present in Lafora disease, a fatal form of progressive myoclonus epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Rubio,Rosa Viana,Mireia Moreno-Estellés,Ángela Campos-Rodríguez,Pascual Sanz

Immunity 55:1799-1812.e4 PubMed36070769

2022

ABCC1 transporter exports the immunostimulatory cyclic dinucleotide cGAMP.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna H Maltbaek,Stephanie Cambier,Jessica M Snyder,Daniel B Stetson

Cell reports 37:110112 PubMed34863353

2021

A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Jiang,Li Shi,Lukui Cai,Xiaoyu Wang,Jingyan Li,Heng Li,Jiangli Liang,Qin Gu,Guang Ji,Jing Li,Longding Liu,Mingbo Sun

International journal of chronic obstructive pulmonary disease 16:2605-2615 PubMed34556981

2021

Nucleotide-Oligomerizing Domain-1 Activation Exaggerates Cigarette Smoke-Induced Chronic Obstructive Pulmonary-Like Disease in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guangchao Han,Min Li,Junfeng Du,Yang Chen,Chen Xu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IP-10 ELISA Kit (CXCL10)

ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Sandwich ELISA - Mouse IP-10 ELISA Kit (CXCL10) (AB260067)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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