AB317783

Mouse IRAK4 ELISA Kit

SimpleStep
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Mouse IRAK4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IRAK4 with a sensitivity of 11.493 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-1 receptor-associated kinase 4, IRAK-4, Irak4

2 Images

Sandwich ELISA - Mouse IRAK4 ELISA Kit (AB317783)

sELISA

Supplier Data

Sandwich ELISA - Mouse IRAK4 ELISA Kit (AB317783)

Example of mouse IRAK4 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IRAK4 ELISA Kit (AB317783)

Interpolated concentrations of IRAK4 in mouse RAW264.7 cell extract and rat 2.4G2 cell extract. Interpolated concentration of native IRAK4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 mg/mL mouse RAW 267.4 cell extract and 0.5 mg/mL rat 2.4G2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 11.493 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IRAK4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IRAK4 protein in Cell Lysate . Quantitate Mouse IRAK4 with 11.493 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "82 - 92 %", "average": "= 86" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317783-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317783-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317783-CMP03" }, { "size":"20 x 1 Vial", "name":"Mouse IRAK4 Lyophilized Recombinant Protein", "number":"AB317783-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317783-CMP12" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317783-CMP10" }, { "size":"10 x 600 µL", "name":"10X Mouse IRAK4 Capture Antibody", "number":"AB317783-CMP01" }, { "size":"10 x 600 µL", "name":"10X Mouse IRAK4 Detector Antibody", "number":"AB317783-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317783-CMP05" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317783-CMP11" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317783-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317783-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317783-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317783-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317783-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317783-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IRAK4 Lyophilized Recombinant Protein", "number":"AB317783-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317783-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IRAK4 Capture Antibody", "number":"AB317783-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IRAK4 Detector Antibody", "number":"AB317783-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317783-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317783-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317783-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.

Biological function summary

IRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.

Pathways

IRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.

Mutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.

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Product protocols

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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections (By similarity).

See full target information Irak4

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse IRAK4 ELISA Kit

Sandwich ELISA - Mouse IRAK4 ELISA Kit (AB317783)

Sandwich ELISA - Mouse IRAK4 ELISA Kit (AB317783)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Proteins & Peptides

Product promise