AB277080

Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule)

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) is a single-wash 90-min Simplestep used to quantify Mouse JAM-A (Junctional Adhesion Molecule) with a sensitivity of 2.92 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD321, Jam1, Jcam, Jcam1, F11r, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, JAM-1

4 Images

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

sELISA

Supplier Data

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Example of mouse JAM-A standard curve in 1X Cell Extraction Buffer PTR.

The JAM-A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Example of mouse JAM-A standard curve in Sample Diluent NS.

The JAM-A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Interpolated concentrations of native JAM-A in mouse placenta based on a 25 µg/mL extract load, lung based on a 25 µg/mL extract load, and spleen based on a 50 µg/mL extract load.

The concentrations of JAM-A were measured in duplicate and interpolated from the JAM-A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean JAM-A concentration was determined to be 1159.97 pg/mL in placenta extract, 2151.45 pg/mL in lung extract, and 1030.16 pg/mL in spleen extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Interpolated concentrations of native JAM-A in mouse serum and plasma samples.

The concentrations of JAM-A were measured in duplicates, interpolated from the JAM-A standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean JAM-A concentration was determined to be 2571.47 pg/mL in serum, 1804.58 pg/mL in plasma (citrate), 1825.22 pg/mL in plasma (EDTA), and 2347.57 pg/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.92 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse JAM-A ELISA kit (ab277080) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse JAM-A protein in mouse serum, plasma, cell culture media, and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse JAM-A with 2.92 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Junction Adhesion Molecule (JAM-A), a type I transmembrane protein, appears to play a role in epithelial tight junction formation. JAM-A emerges early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM-A, thereby preventing tight junction assembly. JAM-A plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. JAM-A shares 68.4%, 91.3%, 96.3%, and 70.1% sequence homology with human, rat, bovine, and monkey, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 97 %", "average": "= 89" }, { "sample": "Serum", "range": "82 - 97 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "88 - 110 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "81 - 101 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "81 - 103 %", "average": "= 89" }, { "sample": "Tissue", "range": "80 - 104 %", "average": "= 94" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277080-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277080-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277080-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB277080-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB277080-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277080-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277080-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277080-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277080-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse JAM-A (Junctional Adhesion Molecule) Detector Antibody", "number":"AB277080-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse JAM-A (Junctional Adhesion Molecule) Capture Antibody", "number":"AB277080-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse JAM-A (Junctional Adhesion Molecule) Lyophilized Recombinant Protein", "number":"AB277080-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.

Biological function summary

JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.

Pathways

JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.

JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.

style

left-column

Product protocols

style

left-column

Target data

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed : 11447115). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (PubMed : 11447115). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (PubMed : 9660867). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (By similarity). Involved in platelet activation (By similarity).

See full target information F11r

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 25:10 PubMed36703228

2023

Tumor necroptosis-mediated shedding of cell surface proteins promotes metastasis of breast cancer by suppressing anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoshan Liu,Swati Choksi,Hyung-Joon Kwon,Delong Jiao,Chengyu Liu,Zheng-Gang Liu

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule)

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Sandwich ELISA - Mouse JAM-A ELISA Kit (Junctional Adhesion Molecule) (AB277080)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise