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AB119596

Mouse KIM1 ELISA Kit (TIM-1)

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(12 Publications)

Mouse KIM1 ELISA Kit (TIM-1) is a sandwich ELISA designed to quantify Mouse KIM1 (TIM-1) with a sensitivity of 2 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 31.2 - 2000 pg/mL

View Alternative Names

CD365, Kim1, Tim1, Timd1, Havcr1, Hepatitis A virus cellular receptor 1 homolog, HAVcr-1, Kidney injury molecule 1, T cell immunoglobulin and mucin domain-containing protein 1, T cell membrane protein 1, T-cell immunoglobulin mucin receptor 1, KIM-1, TIMD-1, TIM-1

1 Images
Sandwich ELISA - Mouse KIM1 ELISA Kit (TIM-1) (AB119596)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse KIM1 ELISA Kit (TIM-1) (AB119596)

Representative Standard Curve for ab119596

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

31.2 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

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Product details

Mouse KIM1 ELISA Kit (TIM-1) ab119596 is a sandwich ELISA to measure Mouse KIM1 (TIM-1) in serum, EDTA plasma, heparin plasma, cell culture supernatant, urine with a sensitivity of 2 pg/ml.

How the assay works

A KIM-1 (TIM-1) specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and are incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse KIM-1 (TIM-1) amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse KIM1 ELISA Kit (TIM-1) ab119596 protocol summary

1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm

Get results in 90 minutes with our SimpleStep ELISA® kit

Mouse KIM-1 ELISA Kit (TIM1) (ab213477)
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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "87 pg/mL", "standardDeviation": "6.87", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "792 pg/mL", "standardDeviation": "38.8", "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "842 pg/mL", "standardDeviation": "36.2", "coefficientOfVariability": "= 4.3" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "279 pg/mL", "standardDeviation": "14.78", "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "261 pg/mL", "standardDeviation": "14.87", "coefficientOfVariability": "= 5.7" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "85 pg/mL", "standardDeviation": "7.31", "coefficientOfVariability": "= 8.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.
Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein's activity influences cytokine production and this can affect inflammation processes.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1's involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.
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Product protocols

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Target data

Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (PubMed : 21821911, PubMed : 25645598, PubMed : 32668241). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed : 24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed : 24703780). Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (PubMed : 25582854, PubMed : 25645598). Acts as a regulator of T-cell proliferation (PubMed : 15793576). May play a role in kidney injury and repair (By similarity).
See full target information Havcr1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 16: PubMed37631096

2023

Protective Role of Betulinic Acid against Cisplatin-Induced Nephrotoxicity and Its Antibacterial Potential toward Uropathogenic Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemah A Alherz,Engy Elekhnawy,Hend Mostafa Selim,Thanaa A El-Masry,Aya H El-Kadem,Ismail A Hussein,Walaa A Negm

Molecular therapy : the journal of the American Society of Gene Therapy 31:344-361 PubMed36514292

2022

Treatment with quercetin inhibits SARS-CoV-2 N protein-induced acute kidney injury by blocking Smad3-dependent G1 cell-cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Wu,Wenbiao Wang,Liying Liang,Junzhe Chen,Biao Wei,Xiao-Ru Huang,Xiaoqin Wang,Xueqing Yu,Hui-Yao Lan

Nutrients 14: PubMed35215435

2022

Blood DNA Methylation Predicts Diabetic Kidney Disease Progression in High Fat Diet-Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Long T Nguyen,Benjamin P Larkin,Rosy Wang,Alen Faiz,Carol A Pollock,Sonia Saad

Antioxidants (Basel, Switzerland) 10: PubMed34943023

2021

Nephroprotective Effects of Synthetic Flavonoid Hidrosmin in Experimental Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Luna Jiménez-Castilla,Gema Marín-Royo,Macarena Orejudo,Lucas Opazo-Ríos,Teresa Caro-Ordieres,Inés Artaiz,Tatiana Suárez-Cortés,Arturo Zazpe,Gonzalo Hernández,Carmen Gómez-Guerrero,Jesús Egido

American journal of physiology. Renal physiology 321:F135-F148 PubMed34151589

2021

KIM-1-mediated anti-inflammatory activity is preserved by MUC1 induction in the proximal tubule during ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad M Al-Bataineh,Carol L Kinlough,Zaichuan Mi,Edwin K Jackson,Stephanie M Mutchler,David R Emlet,John A Kellum,Rebecca P Hughey

American journal of physiology. Endocrinology and 317:E269-E283 PubMed31039005

2019

Hydrogen sulfide inhibits Ca-induced mitochondrial permeability transition pore opening in type-1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

A Sashi Papu John,Sourav Kundu,Sathnur Pushpakumar,Matthew Amin,Suresh C Tyagi,Utpal Sen

Journal of the American Association for Laboratory Animal Science : JAALAS 58:21-29 PubMed30538007

2018

Effects of General Anesthesia on 2 Urinary Biomarkers of Kidney Injury-Hepatitis A Virus Cellular Receptor 1 and Lipocalin 2-in Male C57BL/6J Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Krista M Gibbs,Jenelle M Izer,W Brian Reeves,Ronald P Wilson,Timothy K Cooper

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:2212-2227 PubMed30247986

2018

Precision toxicology shows that troxerutin alleviates ochratoxin A-induced renal lipotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yang,Wentao Xu,Kunlun Huang,Boyang Zhang,Haomiao Wang,Xueqin Zhang,Lijing Gong,Yunbo Luo,Xiaoyun He

Toxicology letters 299:226-235 PubMed30236763

2018

Sema 3A as a biomarker of the activated mTOR pathway during hexavalent chromium-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Fang Song,Yuan Yang,Zong-Wei Yi,Zai-Qi Zhang,Xiang-Dong Shen,Gong-Hua Hu,Yong-Fei Zhu

British journal of haematology 181:111-121 PubMed29527679

2018

Renal protection by atorvastatin in a murine model of sickle cell nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Rima S Zahr,Prasanthi Chappa,Hong Yin,Lou A Brown,Kenneth I Ataga,David R Archer
View all publications
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