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AB155448

Mouse L-Selectin ELISA Kit (CD62L)

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(1 Publication)

Mouse L-Selectin ELISA Kit (CD62L) is a Sandwich ELISA for the measurement of Mouse L-Selectin (CD62L) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD62L, Lnhr, Ly-22, Ly22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1

1 Images
Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (AB155448)
  • sELISA

Unknown

Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (AB155448)

L-Selectin Mouse ELISA Kit.

Representative standard curve using ab155448

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 35 pg/mL

Range

40.96 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's L-Selectin (CD62L) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse L-Selectin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse L-Selectin (CD62L) coated on a 96-well plate. Standards and samples are pipetted into the wells and L-Selectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse L-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of L-Selectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Mouse L-Selectin ELISA Kit (CD62L) (ab199085) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 111 %", "average": "= 103.1" }, { "sample": "Plasma", "range": "82 - 104 %", "average": "= 95.69" }, { "sample": "Cell culture media", "range": "103 - 116 %", "average": "= 109.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155448-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155448-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155448-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"L-Selectin Microplate (12 x 8 wells)", "number":"AB155448-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse L-Selectin Standard (lyophilized)", "number":"AB155448-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155448-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155448-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB155448-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB155448-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse L-Selectin", "number":"AB155448-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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Product protocols

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Target data

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed : 1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).
See full target information Sell
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 11:419 PubMed32322204

2020

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Applications

Unspecified application

Species

Unspecified reactive species

Daniel P Zimmer,Courtney M Shea,Jenny V Tobin,Boris Tchernychev,Peter Germano,Kristie Sykes,Ali R Banijamali,Sarah Jacobson,Sylvie G Bernier,Renee Sarno,Andrew Carvalho,Yueh-Tyng Chien,Regina Graul,Emmanuel S Buys,Juli E Jones,James D Wakefield,Gavrielle M Price,Jennifer G Chickering,G Todd Milne,Mark G Currie,Jaime L Masferrer
View all publications
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websiteProtocolBooklet
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Product promise

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