Mouse L-Selectin ELISA Kit (CD62L) is a single-wash 90-min Simplestep used to quantify Mouse L-Selectin (CD62L) with a sensitivity of 1.07 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.07 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed:1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).
Alternative names
CD62L, Lnhr, Ly-22, Ly22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1
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Mouse L-Selectin ELISA Kit (CD62L) is a single-wash 90-min Simplestep used to quantify Mouse L-Selectin (CD62L) with a sensitivity of 1.07 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 1.07 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 7.56 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.55 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 96 | Range 95 - 97 % |
Sample type Citrate plasma | Average % = 97 | Range 95 - 99 % |
Sample type Cell culture media | Average % = 96 | Range 93 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse L-Selectin ELISA Kit (CD62L) (ab199085) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of L-Selectin (CD62L) protein in cell culture supernatant, cit plasma, serum, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse L-Selectin (CD62L) with 1.07 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
L-Selectin is a cell surface glycoprotein and a member of a family of adhesion proteins. L-Selectin was first identified in mice as the Mel-14 antigen, a lymph node homing receptor also found on neutrophils and monocytes expressed on a variety of leukocytes. This molecule acts as adhesion and homing receptor that plays an important role in lymphocyte-endothelial cell recognition and migration. The molecule itself is composed of multiple domains: 1 homologous to lectins, 1 to epidermal growth factor, and 2 to the consensus repeat units found in C3/C4 binding proteins.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
- Biological function summary
CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.
- Pathways
CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.
- Associated diseases and disorders
CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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4 product images
Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (ab199085)
Example of L-Selectin standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (ab199085)
Example of L-Selectin standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (ab199085)
Demonstration of linearity of dilution of the assay.
Titration of mouse serum and citrate plasma within the working range of the assay. The 1X dilution for mouse serum and plasma was 0.1% matrix. The interpolated L-Selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).
Sandwich ELISA - Mouse L-Selectin ELISA Kit (CD62L) (ab199085)
Titration of stimulated and unstimulated splenocyte supernatant within the working range of the assay.
The 1X dilution for both the stimulated and unstimulated supernatants was 100% matrix. The interpolated L-Selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).
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