Mouse L1CAM ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse L1CAM with a sensitivity of 42.76 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 42.76 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Alternative names
CD171, Caml1, L1cam, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1
What's included?
Recommended products
Mouse L1CAM ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse L1CAM with a sensitivity of 42.76 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 42.76 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 4.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 110 | Range 101 - 120 % |
Sample type EDTA Plasma | Average % = 119 | Range 113 - 125 % |
Sample type Heparin Plasma | Average % = 114 | Range 111 - 117 % |
Sample type Citrate plasma | Average % = 105 | Range 88 - 117 % |
Sample type Cell Lysate | Average % = 110 | Range 103 - 121 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse L1CAM ELISA kit (ab282313) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse L1CAM protein in mouse serum, plasma, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse L1CAM with 42.76 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Neural cell adhesion molecule L1 (L1CAM) is involved in dynamics of cell adhesion and generation of transmembrane signals at tyrosine kinase receptors. Studies suggest L1CAM functions during mouse brain development. L1CAM is critical neuronal migration, axonal growth and fasciculation, and synaptogenesis processes. In the mature brain, L1CAM plays a role in the dynamics of neuronal structure and function, including synaptic plasticity. Mouse L1CAM shows 88%, 88%, 99% and 89% sequence homology with human, rat, monkey, and cow, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.
- Biological function summary
L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.
- Pathways
L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.
- Associated diseases and disorders
L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Mouse L1CAM ELISA Kit (ab282313)
Example of mouse L1CAM standard curve in Sample Diluent NS.
The L1CAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse L1CAM ELISA Kit (ab282313)
Interpolated concentrations of native L1CAM in mouse serum and plasma samples.
The concentrations of L1CAM were measured in duplicates, interpolated from the L1CAM standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 537.14 ng/mL in serum, 423.28 ng/mL in plasma (citrate), 373.69 ng/mL in plasma (EDTA), and 355.32 ng/mL in plasma (heparin).
Sandwich ELISA - Mouse L1CAM ELISA Kit (ab282313)
Interpolated concentrations of native L1CAM in mouse heart, lung and brain extract based on a 200 μg/mL extract load for heart and lung extract and a 6.25 μg/mL extract load for brain extract samples.
The concentrations of L1CAM were measured in duplicate and interpolated from the L1CAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 1459.6 pg/mL in lung extract samples, 1328.94 in heart extract samples and 5647.08 pg/mL in brain extract samples.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com