Mouse Laminin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Laminin in Mouse in Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids samples.
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- Redox biology 56:1024352022Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihua Li,Kunpeng Wang,Rongjun Jia,Jing Xie,Liman Ma,Zhiqing Hao,Weiwei Zhang,Jinggang Mo,Fu RenPubMed 36029649
- Biomaterials 273:1208082021In vitro generation of peri-islet basement membrane-like structures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJorge Santini-González,Jennifer A Simonovich,Roberto Castro-Gutiérrez,Yarelis González-Vargas,Nicholas J Abuid,Cherie L Stabler,Holger A Russ,Edward A PhelpsPubMed 33895491
- Scientific reports 9:193032019The Adult Murine Intestine is Dependent on Constitutive Laminin-γ1 Synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBritish Fields et. al.PubMed 31848396
- Biomaterials science 7:5338-53492019Engineering hydrogels with affinity-bound laminin as 3D neural stem cell culture systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Barros,Eduardo Conde-Sousa,Andreia M Gonçalves,Woojin M Han,Andrés J García,Isabel F Amaral,Ana P PêgoPubMed 31620727
- Molecular medicine reports 19:1318-13302018Melatonin attenuates renal fibrosis in diabetic mice by activating the AMPK/PGC1α signaling pathway and rescuing mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Li,Nan Li,Shuangtong Yan,Yanhui Lu,Xinyu Miao,Zhaoyan Gu,Yinghong ShaoPubMed 30535482
- ACS applied materials & interfaces 10:5305-53172018Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156 - 10000 pg/mL
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Additional Targets
Lamb1, Lamc1
Alternative names
Lama, Lama-1, Lama1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha
What's included?
Recommended products
Mouse Laminin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Laminin in Mouse in Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156 - 10000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 10 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's mouse Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Laminin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
A polyclonal antibody from rabbit specific for Laminin has been precoated onto 96-well plates. Standards(from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from rabbit specific for Laminin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Laminin amount of sample captured in plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.
- Biological function summary
Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.
- Pathways
Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.
- Associated diseases and disorders
Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.
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1 product image
Sandwich ELISA - Mouse Laminin ELISA Kit (ab119572)
Representative Standard Curve using ab119572.
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