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AB119572

Mouse Laminin ELISA Kit

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(6 Publications)

Mouse Laminin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Laminin in Mouse in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

Lama, Lama-1, Lama1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha, Lamb-1, Lamb1-1, Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, Lamb-2, Lamc-1, Laminin subunit gamma-1, Laminin B2 chain, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, S-laminin subunit gamma, S-LAM gamma

1 Images
Sandwich ELISA - Mouse Laminin ELISA Kit (AB119572)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Laminin ELISA Kit (AB119572)

Representative Standard Curve using ab119572.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Primary Antibodies

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KO Validated

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's mouse Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Laminin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

A polyclonal antibody from rabbit specific for Laminin has been precoated onto 96-well plates. Standards(from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from rabbit specific for Laminin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Laminin amount of sample captured in plate.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119572-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119572-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse Laminin antibody", "number":"AB119572-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse Laminin Antibody Microplate (12 x 8 wells)", "number":"AB119572-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119572-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119572-CMP10", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119572-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119572-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized Recombinant mouse Laminin standard", "number":"AB119572-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119572-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119572-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119572-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.
Biological function summary

Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.

Pathways

Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.

Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.
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Product protocols

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information Lama1

Additional targets

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 56:102435 PubMed36029649

2022

Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Li,Kunpeng Wang,Rongjun Jia,Jing Xie,Liman Ma,Zhiqing Hao,Weiwei Zhang,Jinggang Mo,Fu Ren

Biomaterials 273:120808 PubMed33895491

2021

In vitro generation of peri-islet basement membrane-like structures.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Santini-González,Jennifer A Simonovich,Roberto Castro-Gutiérrez,Yarelis González-Vargas,Nicholas J Abuid,Cherie L Stabler,Holger A Russ,Edward A Phelps

Scientific reports 9:19303 PubMed31848396

2019

The Adult Murine Intestine is Dependent on Constitutive Laminin-γ1 Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

British Fields,Ann DeLaForest,Mark Zogg,Jennifer May,Catherine Hagen,Kristin Komnick,Jon Wieser,Alexander Lundberg,Hartmut Weiler,Michele A Battle,Karen-Sue Carlson

Biomaterials science 7:5338-5349 PubMed31620727

2019

Engineering hydrogels with affinity-bound laminin as 3D neural stem cell culture systems.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Barros,Eduardo Conde-Sousa,Andreia M Gonçalves,Woojin M Han,Andrés J García,Isabel F Amaral,Ana P Pêgo

Molecular medicine reports 19:1318-1330 PubMed30535482

2018

Melatonin attenuates renal fibrosis in diabetic mice by activating the AMPK/PGC1α signaling pathway and rescuing mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Nan Li,Shuangtong Yan,Yanhui Lu,Xinyu Miao,Zhaoyan Gu,Yinghong Shao

ACS applied materials & interfaces 10:5305-5317 PubMed29381329

2018

Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications.

Applications

ELISA

Species

Unspecified reactive species

Chia-Chen Hsu,Andrea Serio,Nadav Amdursky,Cyril Besnard,Molly M Stevens
View all publications
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Product promise

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