Mouse LBP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse LBP in Mouse in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
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- iScience 25:1037072022Enterocyte superoxide dismutase 2 deletion drives obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOihane Garcia-Irigoyen,Fabiola Bovenga,Marilidia Piglionica,Elena Piccinin,Marica Cariello,Maria Arconzo,Claudia Peres,Paola Antonia Corsetto,Angela Maria Rizzo,Marta Ballanti,Rossella Menghini,Geltrude Mingrone,Philippe Lefebvre,Bart Staels,Takuji Shirasawa,Carlo Sabbà,Gaetano Villani,Massimo Federici,Antonio MoschettaPubMed 35036884
- NPJ Parkinson's disease 8:92022Enteric α-synuclein impairs intestinal epithelial barrier through caspase-1-inflammasome signaling in Parkinson's disease before brain pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Pellegrini,V D'Antongiovanni,F Miraglia,L Rota,L Benvenuti,C Di Salvo,G Testa,S Capsoni,G Carta,L Antonioli,A Cattaneo,C Blandizzi,E Colla,M FornaiPubMed 35022395
- Foods (Basel, Switzerland) 10:2021Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich Polysaccharides on the High-Fat Diet-Fed Mice Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinglei Yu,Jin Yue,Nan Hui,Yuee Zhi,Kashif Hayat,Xijia Yang,Dan Zhang,Shaohua Chu,Pei ZhouPubMed 34681302
- Cellular and molecular gastroenterology and hepatology 12:891-9202021Nonalcoholic Steatohepatitis and HCC in a Hyperphagic Mouse Accelerated by Western Diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSouradipta Ganguly,German Aleman Muench,Linshan Shang,Sara Brin Rosenthal,Gibraan Rahman,Ruoyu Wang,Yanhan Wang,Hyeok Choon Kwon,Anthony M Diomino,Tatiana Kisseleva,Pejman Soorosh,Mojgan Hosseini,Rob Knight,Bernd Schnabl,David A Brenner,Debanjan DharPubMed 34062281
- Scientific reports 9:121872019Cocaine Induces Inflammatory Gut Milieu by Compromising the Mucosal Barrier Integrity and Altering the Gut Microbiota Colonization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErnest T Chivero,Rizwan Ahmad,Annadurai Thangaraj,Palsamy Periyasamy,Balawant Kumar,Elisa Kroeger,Dan Feng,Ming-Lei Guo,Sabita Roy,Punita Dhawan,Amar B Singh,Shilpa BuchPubMed 31434922
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.56 - 100 ng/mL
- Assay time
- 3h 30m
- Sensitivity
- < 50 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:9144073). Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity). Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:24380872).
Alternative names
Lipopolysaccharide-binding protein, LBP, Lbp
What's included?
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Mouse LBP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse LBP in Mouse in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.56 - 100 ng/mL
- Assay time
- 3h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 50 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 16 | mean 3.313 ng/mL | SD 0.237 | C.V. 7.1 |
Sample Sample 2 | n 16 | mean 12.997 ng/mL | SD 0.832 | C.V. 6.4 |
Sample Sample 3 | n 16 | mean 39.089 ng/mL | SD 2.111 | C.V. 5.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 24 | mean 3.385 ng/mL | SD 0.244 | C.V. 7.2 |
Sample Sample 2 | n 24 | mean 14.212 ng/mL | SD 1.151 | C.V. 8.1 |
Sample Sample 3 | n 24 | mean 39.495 ng/mL | SD 2.804 | C.V. 7.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213876) is designed for the quantitative detection of Mouse LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for LBP has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: G25-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse LBP amount of sample captured in plate.
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS dependent monocyte responses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.
- Biological function summary
LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.
- Pathways
LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.
- Associated diseases and disorders
Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.
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Sandwich ELISA - Mouse LBP ELISA Kit (ab213876)
Mouse LBP ELISA Kit (ab213876) Standard Curve
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