JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB269542

Mouse LBP ELISA Kit (LPS Binding Protein)

5

(1 Review)

|

(18 Publications)

Mouse LBP ELISA Kit (LPS Binding Protein) is a single-wash 90-min Simplestep used to quantify Mouse LBP (LPS Binding Protein) with a sensitivity of 5.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

Lipopolysaccharide-binding protein, LBP, Lbp

3 Images
ELISA - Mouse LBP ELISA Kit (LPS Binding Protein) (AB269542)
  • ELISA

Supplier Data

ELISA - Mouse LBP ELISA Kit (LPS Binding Protein) (AB269542)
Sandwich ELISA - Mouse LBP ELISA Kit (LPS Binding Protein) (AB269542)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LBP ELISA Kit (LPS Binding Protein) (AB269542)

Interpolated concentrations of native LBP in mouse serum and plasma samples.

The concentrations of LBP were measured in duplicates, interpolated from the LBP standard curves and corrected for sample dilution. Undiluted samples referred to suggested minimum dilution as follows : serum 1 : 1,000, plasma (citrate) 1 : 2,000, plasma (heparin) 1 : 1,000, and plasma (EDTA) 1 : 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LBP concentration was determined to be 1.06 μg/mL in serum, 2.37 μg/mL in plasma (citrate), 1.53 μg/mL in plasma (heparin), and 2.12 μg/mL in plasma (EDTA).

Sandwich ELISA - Mouse LBP ELISA Kit (LPS Binding Protein) (AB269542)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LBP ELISA Kit (LPS Binding Protein) (AB269542)

Example of mouse LBP standard curve in Sample Diluent NS.

The LBP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 5.4 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse LBP ELISA Kit (LPS Binding Protein) ab269542 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse LBP (LPS Binding Protein)in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 5.4 pg/mL.

How the assay works

Mouse LBP (LPS Binding Protein)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse LBP ELISA Kit (LPS Binding Protein) ab269542 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "83 - 85 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "96 - 99 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "92 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "95 - 104 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "101 - 105 %", "average": "= 103" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269542-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB269542-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse LBP (LPS Binding Protein) Lyophilized Recombinant Protein", "number":"AB269542-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269542-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LBP (LPS Binding Protein) Capture Antibody", "number":"AB269542-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269542-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269542-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269542-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LBP (LPS Binding Protein) Detector Antibody", "number":"AB269542-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269542-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269542-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.
Biological function summary

LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.

Pathways

LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.

Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.
style
left-column

Product protocols

style
left-column

Target data

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed : 9144073). Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity). Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed : 24380872).
See full target information Lbp
style
left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Inflammation : PubMed40526227

2025

SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peixuan Zhu,Yujie Wu,Yateng Sun,Yonghuang Yan,Yanmin Wang,Yuling Yu,Jiusi Yang,Yuhan Wang,Zishuo Guo,Siqi Wang,Cai Zhang,Zeqi Su

Food & function 16:2389-2400 PubMed39996301

2025

Gut microbiota modulation and inflammation mitigation in a murine model through a hull-less and purple grain barley genotype.

Applications

Unspecified application

Species

Unspecified reactive species

María-Engracia Cortijo-Alfonso,Houda Laghouaouta,Ramona N Pena,Mariona Martínez,Silvia Yuste,Laura Rubió-Piqué,Carme Piñol-Felis

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 22:e00494 PubMed39580323

2024

Abdominal ultrasound stimulation alleviates DSS-induced colitis and behavioral disorders in mice by mediating the microbiota-gut-brain axis balance.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Yong Gao,Yi-Ju Pan,Wei-Shen Su,Chun-Yi Wu,Ting-Yu Chang,Feng-Yi Yang

Nutrition & diabetes 14:46 PubMed38902253

2024

Dietary resistant starch enhances immune health of the kidney in diabetes via promoting microbially-derived metabolites and dampening neutrophil recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Snelson,Devy Deliyanti,Sih Min Tan,Anna M Drake,Cassandra de Pasquale,Vinod Kumar,Trent M Woodruff,Jennifer L Wilkinson-Berka,Melinda T Coughlan

Microbiology spectrum 12:e0197923 PubMed38647315

2024

Ling-Gui-Zhu-Gan decoction ameliorates nonalcoholic fatty liver disease via modulating the gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Ping Chen,Lin-Fang Zhang,Shuang Liu,Hua Hua,Lei Zhang,Bao-Cheng Liu,Rui-Rui Wang

Gut microbes 15:2281014 PubMed37988132

2023

Gut-microbiota prompt activation of natural killer cell on alcoholic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jung A Eom,Jin-Ju Jeong,Sang Hak Han,Goo Hyun Kwon,Kyeong Jin Lee,Haripriya Gupta,Satya Priya Sharma,Sung-Min Won,Ki-Kwang Oh,Sang Jun Yoon,Hyun Chae Joung,Kyung Hwan Kim,Dong Joon Kim,Ki Tae Suk

Nutrients 15: PubMed37892502

2023

Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of Butyrate.

Applications

Unspecified application

Species

Unspecified reactive species

He Xia,Jing Guo,Jian Shen,Shiman Jiang,Shengyi Han,Lanjuan Li

Nutrients 15: PubMed37836540

2023

Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Modulating the Gut Microbiota in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Shen,Shuting Wang,Yong Huang,Zhengjie Wu,Shengyi Han,He Xia,Hui Chen,Lanjuan Li

Nutrients 15: PubMed37111020

2023

Nobiletin Improves D-Galactose-Induced Aging Mice Skeletal Muscle Atrophy by Regulating Protein Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Hui Wang,Yan Zhang,Tai-Qi Qu,Xue-Qin Sang,Yi-Xuan Li,Fa-Zheng Ren,Peng-Cheng Wen,Ya-Nan Sun

Aging cell 22:e13786 PubMed36704918

2023

Bifidobacterium longum supplementation improves age-related delays in fracture repair.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph L Roberts,Mateo Golloshi,Derek B Harding,Madison Conduah,Guanglu Liu,Hicham Drissi
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB197742

Mouse IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

  • 5
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB222511

Mouse C Reactive Protein ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse C Reactive Protein ELISA Kit (AB222511)

  • 0
  • 0
Assay Kits

AB222503

Mouse IL-6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com