Mouse LDLR ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LDLR with a sensitivity of 0.035 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Cell culture media, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.086 - 5.5 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.035 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization.
Alternative names
Low-density lipoprotein receptor, LDL receptor, Ldlr
What's included?
Mouse LDLR ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LDLR with a sensitivity of 0.035 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Cell culture media, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.086 - 5.5 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.035 ng/mL
- General recovery
- 111%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 2.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 4.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 106 - 120 % |
Sample type Citrate plasma | Average % = 99 | Range 95 - 102 % |
Sample type Heparin Plasma | Average % = 106 | Range 100 - 116 % |
Sample type Cell culture media | Average % = 86 | Range 83 - 92 % |
Sample type Tissue Extracts | Average % = 109 | Range 102 - 114 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse LDLR SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LDLR protein in Serum, Cit Plasma, Hep Plasma, Cell culture media, Tissue extracts. Quantitate Mouse LDLR with 0.035 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Mouse LDLR ELISA Kit (ab323525)
Mouse LDLR competitor standard curve comparison.
Standard curve comparison between Mouse LDLR SimpleStep ELISA kit (ab323525) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Mouse LDLR ELISA Kit (ab323525)
Interpolated concentrations of mouse LDLR in mouse liver tissue extract in 96-well vs. 384-well plates.
Interpolated concentration of native LDLR was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 µg/mL mouse liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Mouse LDLR ELISA Kit (ab323525)
Example of mouse LDLR standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.
Example of mouse LDLR standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse LDLR ELISA Kit (ab323525)
Interpolated concentrations of mouse LDLR in mouse serum, plasma (citrate), and plasma (heparin).
Interpolated concentration of native LDLR was measured in duplicate at different sample concentrations: 1:40 mouse serum, 1:40 mouse plasma (citrate), and 1:20 mouse plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Mouse LDLR ELISA Kit (ab323525)
Interpolated concentrations of mouse LDLR in mouse liver tissue extract.
Interpolated concentration of native LDLR was measured in duplicate at 100 µg/mL mouse liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Mouse LDLR ELISA Kit (ab323525)
Example of mouse LDLR standard curve in 1X Cell Extraction Buffer PTR.
Example of mouse LDLR standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
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