AB323525

Mouse LDLR ELISA Kit

SimpleStep
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Mouse LDLR ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LDLR with a sensitivity of 0.035 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Low-density lipoprotein receptor, LDL receptor, Ldlr

6 Images

Sandwich ELISA - Mouse LDLR ELISA Kit (AB323525)

sELISA

Supplier Data

Sandwich ELISA - Mouse LDLR ELISA Kit (AB323525)

Interpolated concentrations of mouse LDLR in mouse liver tissue extract in 96-well vs. 384-well plates.

Interpolated concentration of native LDLR was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 µg/mL mouse liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse LDLR ELISA Kit (AB323525)

Example of mouse LDLR standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of mouse LDLR standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse LDLR ELISA Kit (AB323525)

Mouse LDLR competitor standard curve comparison.

Standard curve comparison between Mouse LDLR SimpleStep ELISA kit (ab323525) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse LDLR ELISA Kit (AB323525)

Interpolated concentrations of mouse LDLR in mouse serum, plasma (citrate), and plasma (heparin).

Interpolated concentration of native LDLR was measured in duplicate at different sample concentrations : 1 : 40 mouse serum, 1 : 40 mouse plasma (citrate), and 1 : 20 mouse plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse LDLR ELISA Kit (AB323525)

Interpolated concentrations of mouse LDLR in mouse liver tissue extract.

Interpolated concentration of native LDLR was measured in duplicate at 100 µg/mL mouse liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse LDLR ELISA Kit (AB323525)

Example of mouse LDLR standard curve in 1X Cell Extraction Buffer PTR.

Example of mouse LDLR standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, Heparin Plasma, Cell culture media, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.035 ng/mL

Range

0.086 - 5.5 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse LDLR SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LDLR protein in Serum, Cit Plasma, Hep Plasma, Cell culture media, Tissue extracts. Quantitate Mouse LDLR with 0.035 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
High sensitivity, specificity and reproducibility from superior antibodies
Fully validated in biological samples
96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]

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Recovery

[ { "sample": "Serum", "range": "106 - 120 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "95 - 102 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "100 - 116 %", "average": "= 106" }, { "sample": "Cell culture media", "range": "83 - 92 %", "average": "= 86" }, { "sample": "Tissue Extracts", "range": "102 - 114 %", "average": "= 109" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323525-10x96Tests", "assetComponentDetails": [ { "size":"10 x 600 µL", "name":"10X Mouse LDLR Detector Antibody", "number":"AB323525-CMP08" }, { "size":"10 x 600 µL", "name":"10X Mouse LDLR Capture Antibody", "number":"AB323525-CMP01" }, { "size":"20 x 1 Vial", "name":"Mouse LDLR Lyophilized Recombinant Protein", "number":"AB323525-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323525-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323525-CMP12" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323525-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323525-CMP06" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323525-CMP05" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323525-CMP11" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB323525-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323525-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323525-CMP04" }, { "size":"1 x 600 µL", "name":"10X Mouse LDLR Detector Antibody", "number":"AB323525-CMP08" }, { "size":"1 x 600 µL", "name":"10X Mouse LDLR Capture Antibody", "number":"AB323525-CMP01" }, { "size":"2 x 1 Vial", "name":"Mouse LDLR Lyophilized Recombinant Protein", "number":"AB323525-CMP09" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323525-CMP12" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323525-CMP03" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323525-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323525-CMP07" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323525-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB323525-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323525-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323525-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LDLR Detector Antibody", "number":"AB323525-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LDLR Capture Antibody", "number":"AB323525-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse LDLR Lyophilized Recombinant Protein", "number":"AB323525-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323525-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323525-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323525-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323525-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323525-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323525-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB323525-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization.

See full target information Low-density lipoprotein receptor

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com