Mouse Legumain ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Mouse Legumain ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Legumain with a sensitivity of 8.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Prsc1, Lgmn, Legumain
- sELISA
Supplier Data
Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)
Interpolated concentrations of native Legumain in mouse serum and spiked Legumain in RPMI Base Media.
The concentrations of Legumain were measured in duplicates, interpolated from the Legumain standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, and RPMI Base Media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Legumain concentration was determined to be 9.1 ng/mL in serum and 1.7 ng/mL in RPMI Base Media 10%.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)
Interpolated concentrations of native Legumain in mouse RAW 264.7 cell extract based on a 600 µg/mL extract load.
The concentrations of Legumain were measured in duplicate and interpolated from the Legumain standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Legumain concentration was determined to be 2.77 ng/mL in mouse RAW 264.7 cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)
Interpolated concentrations of native Legumain in mouse plasma (heparin, EDTA, and citrate).
The concentrations of Legumain were measured in duplicates, interpolated from the Legumain standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (heparin) 5%, plasma (EDTA) 2.5% and plasma (citrate) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Legumain concentration was determined to be 14.1 ng/mL in plasma (heparin), 7.7 ng/mL in plasma (EDTA) and 6.5 ng/mL in plasma (citrate).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)
Example of mouse Legumain standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)
Example of mouse Legumain standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Mouse Legumain ELISA Kit (ab207617) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Legumain protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Legumain with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Legumain is a lysosomal cysteine protease that plays a pivotal role in the endosomal/lysosomal degradation system. Specifically, Legumain is required for the normal degradation of internalized EGFR, allowing Legumain to regulate cell proliferation via this role. Mouse Legumain has 94% and 83% identity to rat and human Legumain, respectively.Legumain has a strong specificity for hydrolysis of asparaginyl bonds, but can also cleave aspartyl bonds slowly, especially under acidic conditions. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Legumain contributes to protein degradation and antigen processing. It does not operate alone as it is part of larger protein complexes which enhance its stability and function. Through its role in proteolysis legumain influences cellular processes like maturation of endolysosomal enzymes and processing of proenzymes. Additionally it assists in activating other proteases signifying its critical position in maintaining cellular proteostasis.
Pathways
Legumain participates in the regulation of immune responses and cellular homeostasis. It plays a significant role in the lysosomal pathway where it partners with cathepsins to assist in the degradation of proteins. Additionally legumain influences the MHC class II pathway by contributing to the cleavage of invariant chain aiding antigen presentation. Its interaction with cathepsins and the impact on antigen processing indicates its necessity in immune system functionality.
Product protocols
- Visit the General protocols
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- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Cell death & disease 11:1014 PubMed33243972
2020
Applications
Unspecified application
Species
Unspecified reactive species
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