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AB235648

Mouse LH Beta ELISA Kit

4

(1 Review)

|

(2 Publications)

Mouse LH Beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LH Beta with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Lutropin subunit beta, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, Lhb

3 Images
Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Example of mouse LH Beta standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Interpolated concentrations of native LH Beta in mouse pituitary extract based on a 6.25 μg/mL extract load.

The concentrations of LH Beta were measured in duplicate and interpolated from the LH Beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LH Beta concentration was determined to be 1.84 ng/mL in mouse pituitary extract.

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LH Beta ELISA Kit (AB235648)

Representative image of Mouse LH Beta ELISA Kit (ab235648)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse LH Beta Capture Antibody

- 10X Mouse LH Beta Detector Antibody

- Mouse LH Beta Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 4.7 pg/mL

Range

117 - 7500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse LH Beta ELISA Kit (ab235648) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LH Beta protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LH Beta with 4.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Pituitary", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": null, "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235648-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235648-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235648-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB235648-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235648-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235648-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235648-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LH Beta Capture Antibody", "number":"AB235648-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB235648-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse LH Beta Lyophilized Recombinant Protein", "number":"AB235648-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB235648-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LH Beta Detector Antibody", "number":"AB235648-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235648-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LHB also known as luteinizing hormone beta or beta LH is a subunit of luteinizing hormone (LH). It plays an important mechanical role in gonadal function. LHB shares a common alpha subunit with other glycoprotein hormones while the beta subunit which is unique to each hormone confers biological specificity. LHB contributes to the dimeric structure of LH giving it a molecular mass around 30 kDa. This protein is expressed mainly in the anterior pituitary gland where it interacts with cells that influence reproductive processes.
Biological function summary

LHB binds with the alpha subunit to form the active LH hormone which importantly stimulates the ovaries in females and testes in males. In females LH promotes the production of estrogen and progesterone facilitating ovulation and menstrual cycles. In males it stimulates Leydig cells leading to testosterone production. LH hormone functions as part of a complex with its receptor a G protein-coupled complex influencing the reproductive system's normal operations.

Pathways

The LHB is central to the hypothalamic-pituitary-gonadal axis a feedback loop that regulates reproductive physiology. Within this axis gonadotropin-releasing hormone (GnRH) from the hypothalamus stimulates LHB production in concert with follicle-stimulating hormone (FSH). These hormones together regulate the development and function of the gonads. LHB's interaction with LH receptors on gonadal tissues signifies its operational involvement in the steroidogenesis and gametogenesis pathways.

Abnormalities in LHB expression associate with conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS altered LHB levels lead to hormonal imbalances affecting fertility. Hypogonadism may result from insufficient LHB affecting sexual development and function. LHB's influence on testosterone and estrogen levels links it closely with disorders characterized by hormonal deficiencies or excesses tying it to complications involving the HPG axis.
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Product protocols

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Target data

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
See full target information Lhb
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40426904

2025

Differential Effects of Canonical Androgens and 11-Ketotestosterone on Reproductive Phenotypes and Folliculogenesis in Mouse Model of PCOS.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ru Tsai,Yen-Nung Liao,Cheng-Ju Tsai,Yu-Ang Lee,Shih-Min Hsia,Kuo-Chung Lan,Hong-Yo Kang

Reproductive biology and endocrinology : RB&E 19:135 PubMed34496874

2021

Irisin alleviates obesity-related spermatogenesis dysfunction via the regulation of the AMPKα signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mu,Huang-Guan Dai,Ling-Bo Luo,Jing Yang
View all publications
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