Mouse LH Beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LH Beta with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Reproductive biology and endocrinology : RB&E 19:1352021Irisin alleviates obesity-related spermatogenesis dysfunction via the regulation of the AMPKα signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Mu,Huang-Guan Dai,Ling-Bo Luo,Jing YangPubMed 34496874
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 117 - 7500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Alternative names
Lutropin subunit beta, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, Lhb
What's included?
Recommended products
Mouse LH Beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LH Beta with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 117 - 7500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.7 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Pituitary | n 8 | C.V. 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 97 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse LH Beta ELISA Kit (ab235648) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LH Beta protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LH Beta with 4.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LHB also known as luteinizing hormone beta or beta LH is a subunit of luteinizing hormone (LH). It plays an important mechanical role in gonadal function. LHB shares a common alpha subunit with other glycoprotein hormones while the beta subunit which is unique to each hormone confers biological specificity. LHB contributes to the dimeric structure of LH giving it a molecular mass around 30 kDa. This protein is expressed mainly in the anterior pituitary gland where it interacts with cells that influence reproductive processes.
- Biological function summary
LHB binds with the alpha subunit to form the active LH hormone which importantly stimulates the ovaries in females and testes in males. In females LH promotes the production of estrogen and progesterone facilitating ovulation and menstrual cycles. In males it stimulates Leydig cells leading to testosterone production. LH hormone functions as part of a complex with its receptor a G protein-coupled complex influencing the reproductive system's normal operations.
- Pathways
The LHB is central to the hypothalamic-pituitary-gonadal axis a feedback loop that regulates reproductive physiology. Within this axis gonadotropin-releasing hormone (GnRH) from the hypothalamus stimulates LHB production in concert with follicle-stimulating hormone (FSH). These hormones together regulate the development and function of the gonads. LHB's interaction with LH receptors on gonadal tissues signifies its operational involvement in the steroidogenesis and gametogenesis pathways.
- Associated diseases and disorders
Abnormalities in LHB expression associate with conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS altered LHB levels lead to hormonal imbalances affecting fertility. Hypogonadism may result from insufficient LHB affecting sexual development and function. LHB's influence on testosterone and estrogen levels links it closely with disorders characterized by hormonal deficiencies or excesses tying it to complications involving the HPG axis.
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2 product images
Sandwich ELISA - Mouse LH Beta ELISA Kit (ab235648)
Example of mouse LH Beta standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse LH Beta ELISA Kit (ab235648)
Interpolated concentrations of native LH Beta in mouse pituitary extract based on a 6.25 μg/mL extract load.
The concentrations of LH Beta were measured in duplicate and interpolated from the LH Beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LH Beta concentration was determined to be 1.84 ng/mL in mouse pituitary extract.
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