AB238261

Mouse LIF ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Mouse LIF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse LIF with a sensitivity of 1.41 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Leukemia inhibitory factor, LIF, Differentiation-stimulating factor, D factor, Lif

3 Images

Sandwich ELISA - Mouse LIF ELISA Kit (AB238261)

sELISA

Supplier Data

Sandwich ELISA - Mouse LIF ELISA Kit (AB238261)

Interpolated concentrations of native LIF in mouse lung supernatant samples. The concentrations of LIF were measured in duplicates, interpolated from the LIF standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse lung supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIF concentration was determined to be 5832.81 pg/mL in mouse lung supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse LIF ELISA Kit (AB238261)

Mouse LIF Competitor Std Curve Comparison.

Standard Curve comparison between Mouse LIF SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse LIF ELISA Kit (AB238261)

Example of mouse LIF standard curve in Sample Diluent NS. The LIF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.41 pg/mL

Range

10.94 - 700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse LIF ELISA Kit (ab238261) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LIF protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LIF with 1.41 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "106 - 119 %", "average": "= 112" }, { "sample": "Serum", "range": "87 - 91 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "88 - 93 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "81 - 86 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "82 - 87 %", "average": "= 90" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238261-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238261-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238261-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238261-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238261-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238261-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB238261-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LIF Capture Antibody", "number":"AB238261-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse LIF Lyophilized Recombinant Protein", "number":"AB238261-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB238261-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse LIF Detector Antibody", "number":"AB238261-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leukemia Inhibitory Factor (LIF) also known as LIF protein plays a critical mechanical role in cell communication and survival. It is a glycoprotein with a molecular weight (LIF MW) generally around 19 kilodaltons. In various tissues particularly in the mouse LIF expression occurs in the bone marrow muscle and notably the nervous system. Besides its presence in normal tissues it shows up in embryonic stem cells where it maintains their pluripotency in culture conditions.

Biological function summary

LIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.

Pathways

The LIF protein is essential in the JAK/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.

LIF contributes to physiological and pathological states. In cancer such as leukemia LIF influences tumor growth and cancer cell survival. The interaction with other cytokines like Interleukin-6 enhances its effect in oncogenic processes. In inflammatory diseases like rheumatoid arthritis LIF's role relates to inflammation and joint destruction. Understanding its interaction with inflammatory mediators can aid in targeted therapeutic strategies.

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Product protocols

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Target data

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

See full target information Lif

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37569800

2023

The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhao,Bethany N Radford,Mark Ungrin,Wendy Dean,Myriam Hemberger

PLoS biology 21:e3001746 PubMed37134077

2023

Tumor-derived interleukin-1α and leukemia inhibitory factor promote extramedullary hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Derek A G Barisas,Ashraf Ul Kabir,Jun Wu,Karen Krchma,Minseo Kim,Madhav Subramanian,Bernd H Zinselmeyer,Colin L Stewart,Kyunghee Choi

iScience 26:106470 PubMed37091244

2023

An inducible explant model of osteoclast-osteoprogenitor coordination in exacerbated osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jarred M Whitlock,Luis F de Castro,Michael T Collins,Leonid V Chernomordik,Alison M Boyce

Cancer immunology research 10:437-452 PubMed35181779

2022

Overproduction of IFNγ by Cbl-b-Deficient CD8+ T Cells Provides Resistance against Regulatory T Cells and Induces Potent Antitumor Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

SeongJun Han,Zhe Qi Liu,Douglas C Chung,Michael St Paul,Carlos R Garcia-Batres,Azin Sayad,Alisha R Elford,Matthew J Gold,Natasha Grimshaw,Pamela S Ohashi

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse LIF ELISA Kit

Sandwich ELISA - Mouse LIF ELISA Kit (AB238261)

Sandwich ELISA - Mouse LIF ELISA Kit (AB238261)

Sandwich ELISA - Mouse LIF ELISA Kit (AB238261)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise