AB119601

Mouse Lipocalin-2 ELISA Kit (NGAL)

Name: Mouse Lipocalin-2 ELISA Kit (NGAL) (ab119601) | Abcam
Brand: Abcam Limited
SKU: AB119601
Price: 780 USD
Availability: InStock

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(13 Publications)

Mouse Lipocalin-2 ELISA Kit (NGAL) is a sandwich ELISA designed to quantify Mouse Lipocalin-2 (NGAL) with a sensitivity of 10 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78 - 5000 pg/mL
- Cited in over 10 publications

View Alternative Names

Neutrophil gelatinase-associated lipocalin, NGAL, Lipocalin-2, Oncogene 24p3, SV-40-induced 24p3 protein, Siderocalin LCN2, p25, 24p3, Lcn2

4 Images

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Representative Standard Curve using ab119601.

sELISA

Lab

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Mouse lipocalin-2 measured in tissue lysates (10-50 ug protein per mL tested; presented as NGAL per mg of extracted protein; duplicates +/- SD).

sELISA

Lab

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Lipocalin-2 measured in culture supernatants (dilution range 1/1-1/25) from control (-) or activated J774 cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and from RAW264 cells stimulated for 24 hours with 50 ng x mL^-1 of PMA (ab120297) with the addition of 1 ug x mL^-1 of LPS (Sigma) for the last 6 hours (PMA/LPS) (duplicates +/- SD).

sELISA

Lab

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Lipocalin-2 measured in biological fluids (dilution range 1/50-1/500; duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

78 - 5000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Lipocalin-2 ELISA Kit (NGAL) ab119601 is a sandwich ELISA to measure Mouse Lipocalin-2 (NGAL) in serum, EDTA plasma, heparin plasma, cell culture supernatant, urine with a sensitivity of 10 pg/ml.

How the assay works

A Lipocalin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Lipocalin-2 amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Lipocalin-2 ELISA Kit (NGAL) ab119601 protocol summary

1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm

Get results in 90 minutes with our SimpleStep ELISA^® kit

Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "2588 pg/mL", "standardDeviation": "113.87", "coefficientOfVariability": "= 4.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "141 pg/mL", "standardDeviation": "6.2", "coefficientOfVariability": "= 4.4" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "785 pg/mL", "standardDeviation": "38.46", "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "130 pg/mL", "standardDeviation": "7.54", "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "817 pg/mL", "standardDeviation": "54.73", "coefficientOfVariability": "= 6.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "2606 pg/mL", "standardDeviation": "161.57", "coefficientOfVariability": "= 6.2" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119601-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119601-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119601-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse Lipocalin-2 antibody", "number":"AB119601-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse Lipocalin-2 antibody Microplate (12 x 8 wells)", "number":"AB119601-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119601-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119601-CMP10", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB119601-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119601-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119601-CMP07", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse Lipocalin-2 standard", "number":"AB119601-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119601-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 15531878, PubMed : 16446425). Can also bind siderophores from M.tuberculosis (By similarity).

See full target information Lcn2

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Renal physiology 326:F727-F736 PubMed38511219

2024

Parental obesity predisposes offspring to kidney dysfunction and increased susceptibility to ischemia-reperfusion injury in a sex-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Jussara M do Carmo,John E Hall,Xuemei Dai,Nikaela Aitkens,Kylie Larson,Emilio M Luna-Suarez,Zhen Wang,Ana C M Omoto,Alan Mouton,Xuan Li,Luzia N S Furukawa,Viktoria Woronik,Alexandre A da Silva

Pharmaceuticals (Basel, Switzerland) 16: PubMed37631096

2023

Protective Role of Betulinic Acid against Cisplatin-Induced Nephrotoxicity and Its Antibacterial Potential toward Uropathogenic Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemah A Alherz,Engy Elekhnawy,Hend Mostafa Selim,Thanaa A El-Masry,Aya H El-Kadem,Ismail A Hussein,Walaa A Negm

Aging 14:6467-6480 PubMed36036755

2022

Effect of BBT-877, a novel inhibitor of ATX, on a mouse model of type 1 diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Han Lee,Phyu Phyu Khin,GwangHee Lee,Oh Kyung Lim,Hee-Sook Jun

Drug delivery 29:1848-1862 PubMed35708451

2022

Aqueous core epigallocatechin gallate PLGA nanocapsules: characterization, antibacterial activity against uropathogens, and reno-protective effect in cisplatin induced nephrotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Badriyah Alotaibi,Thanaa A El-Masry,Engy Elekhnawy,Aya H El-Kadem,Asmaa Saleh,Walaa A Negm,Dalia H Abdelkader

Experimental and therapeutic medicine 23:303 PubMed35340877

2022

Sevoflurane preconditioning prevents acute renal injury caused by ischemia-reperfusion in mice via activation of the Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Xi Wang,Zhen-Ru Zhao,Ying Bai,Ya-Xing Li,Xiao-Ning Gao,Sen Zhang,Yan-Bin Sun

Journal of inflammation research 14:7035-7051 PubMed34955649

2021

OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fangchen Gong,Ranran Li,Xiangtao Zheng,Weiwei Chen,Yanjun Zheng,Zhitao Yang,Ying Chen,Hongping Qu,Enqiang Mao,Erzhen Chen

JCI insight 6: PubMed34806647

2021

DNA damage is overcome by TRIP13 overexpression during cisplatin nephrotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Taketsugu Hama,Prashanth Kb Nagesh,Pallabita Chowdhury,Bob M Moore,Murali M Yallapu,Kevin R Regner,Frank Park

American journal of physiology. Renal physiology 321:F135-F148 PubMed34151589

2021

KIM-1-mediated anti-inflammatory activity is preserved by MUC1 induction in the proximal tubule during ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad M Al-Bataineh,Carol L Kinlough,Zaichuan Mi,Edwin K Jackson,Stephanie M Mutchler,David R Emlet,John A Kellum,Rebecca P Hughey

Clinical and translational medicine 11:e339 PubMed33634985

2021

CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbin Gong,Jiafei Yu,Tao Zheng,Peizhao Liu,Fan Zhao,Juanhan Liu,Zhiwu Hong,Huajian Ren,Guosheng Gu,Gefei Wang,Xiuwen Wu,Yun Zhao,Jianan Ren

International journal of molecular sciences 20: PubMed31842453

2019

Effects of Farnesiferol B on Ischemia-Reperfusion-Induced Renal Damage, Inflammation, and NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Xianjun Fu,Ting Gui,Tianqi Wang,Zhenguo Wang,Gerd A Kullak-Ublick,Zhibo Gai

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Lipocalin-2 ELISA Kit (NGAL)

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB119601)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (13)

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