JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB199083

Mouse Lipocalin-2 ELISA Kit (NGAL)

5

(2 Reviews)

|

(16 Publications)

Mouse Lipocalin-2 ELISA Kit (NGAL) is a single-wash 90-min Simplestep used to quantify Mouse Lipocalin-2 (NGAL) with a sensitivity of 1.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

Neutrophil gelatinase-associated lipocalin, NGAL, Lipocalin-2, Oncogene 24p3, SV-40-induced 24p3 protein, Siderocalin LCN2, p25, 24p3, Lcn2

6 Images
ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)
  • ELISA

Supplier Data

ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)
Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)

Interpolated concentrations of Lipocalin-2 in mouse serum, plasma (citrate), and platelet poor plasma (EDTA).

The concentrations of Lipocalin-2 were measured in duplicate and interpolated from the Lipocalin-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 265.2 ng/mL in mouse serum, 583.8 ng/mL in mouse plasma (Citrate), and 535.2 ng/mL in platelet poor mouse plasma (EDTA).

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)

Example of Lipocalin-2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)
  • sELISA

Lab

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)

Mouse Lipocalin-2 (NGAL) standard curve comparison data.

Standard curve comparison between mouse Lipocalin-2 (NGAL) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 7-fold increase in sensitivity.

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)

Comparison of secreted Lipocalin-2 in media, unstimulated and PMA/PHA-stimulated J774A.1 cells.

J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Raw data from duplicate measurements are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (AB199083)

Comparison of secreted Lipocalin-2 in mouse cell lines with or without stimulation.

L929 and J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. RAW264.7 were grown in the absence (unstimulated) or presence of LPS (stimulated) for 2 days. Cell Culture supernatant was diluted to within the range of the assay, 5-fold (L929 and J774A.1) or 2,000-fold (RAW264.7). Measured values were interpolated from the Lipocalin-2 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. Lipocalin-2 was undetectable in media.

Key facts

Detection method

Colorimetric

Sample types

Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.1 pg/mL

Range

4.69 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

Mouse Lipocalin-2 ELISA Kit (NGAL) ab199083 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Lipocalin-2 (NGAL)in cell culture supernatant, citrate plasma, EDTA plasma, serum, urine. This SimpleStep sensitivity is 1.1 pg/mL.

How the assay works

Mouse Lipocalin-2 (NGAL)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Lipocalin-2 ELISA Kit (NGAL) ab199083 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity and renal development. Mice deficient in Lipocalin-2 appear normal but have increased susceptibility to bacterial infection. The bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Mouse Lipocalin-2 has 81% and 62% protein sequence identity to rat and human Lipocalin-2, respectively.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "106 - 110 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "87 - 120 %", "average": "= 107" }, { "sample": "Urine", "range": "100 - 107 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "98 - 116 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "102 - 107 %", "average": "= 104" }, { "sample": "serum free media", "range": "98 - 103 %", "average": "= 99" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab199083-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB199083-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB199083-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB199083-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Mouse Lipocalin-2 Capture Antibody", "number":"AB199083-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5B", "number":"AB199083-CMP01" }, { "size":"2 x 10 Vial", "name":"Mouse Lipocalin-2 Lyophilized Recombinant Protein", "number":"AB199083-CMP02" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB199083-CMP03" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB199083-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB199083-CMP09" }, { "size":"1 x 6000 µL", "name":"10X Mouse Lipocalin-2 Detector Antibody", "number":"AB199083-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab199083-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB199083-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5B", "number":"AB199083-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB199083-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Lipocalin-2 Capture Antibody", "number":"AB199083-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Lipocalin-2 Lyophilized Recombinant Protein", "number":"AB199083-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Lipocalin-2 Detector Antibody", "number":"AB199083-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB199083-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB199083-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB199083-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB199083-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 15531878, PubMed : 16446425). Can also bind siderophores from M.tuberculosis (By similarity).
See full target information Lcn2
style
left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37174093

2023

c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Liu,Margaret R Smith,Yuezhu Wang,Ralph D'Agostino,Jimmy Ruiz,Thomas Lycan,Gregory L Kucera,Lance D Miller,Wencheng Li,Michael D Chan,Michael Farris,Jing Su,Qianqian Song,Dawen Zhao,Arvind Chandrasekaran,Fei Xing

Aging cell 22:e13786 PubMed36704918

2023

Bifidobacterium longum supplementation improves age-related delays in fracture repair.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph L Roberts,Mateo Golloshi,Derek B Harding,Madison Conduah,Guanglu Liu,Hicham Drissi

Neurobiology of disease 176:105964 PubMed36526090

2022

TNF and IL6/Jak2 signaling pathways are the main contributors of the glia-derived neuroinflammation present in Lafora disease, a fatal form of progressive myoclonus epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Rubio,Rosa Viana,Mireia Moreno-Estellés,Ángela Campos-Rodríguez,Pascual Sanz

Nature metabolism 4:995-1006 PubMed35902645

2022

Systemic induction of senescence in young mice after single heterochronic blood exchange.

Applications

Unspecified application

Species

Unspecified reactive species

Ok Hee Jeon,Melod Mehdipour,Tae-Hwan Gil,Minha Kang,Nicholas W Aguirre,Zachery R Robinson,Cameron Kato,Jessy Etienne,Hyo Gyeong Lee,Fatouma Alimirah,Vighnesh Walavalkar,Pierre-Yves Desprez,Michael J Conboy,Judith Campisi,Irina M Conboy

Gerontology 68:1145-1165 PubMed35576907

2022

Mechanism of circHIPK3-miRNA-124-3p/miRNA-148b-3p-Mediated Inflammatory Responses and Cell Senescence in Candida albicans-Induced Septic Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Han,Wanli Li,Jie Zhang,Yulin Guan,Ying Huang,Xiaoyue Li

Nature communications 12:7333 PubMed34921145

2021

A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yao,Yalan Deng,Yang Zhao,Ying Mei,Yilei Zhang,Xiaoguang Liu,Consuelo Martinez,Xiaohua Su,Roberto R Rosato,Hongqi Teng,Qinglei Hang,Shannon Yap,Dahu Chen,Yumeng Wang,Mei-Ju May Chen,Mutian Zhang,Han Liang,Dong Xie,Xin Chen,Hao Zhu,Jenny C Chang,M James You,Yutong Sun,Boyi Gan,Li Ma

Pharmacological research 175:106030 PubMed34896544

2021

Toll-like receptor 4 mutation protects the kidney from Ang-II-induced hypertensive injury.

Applications

Unspecified application

Species

Unspecified reactive species

Suravi Majumder,Sathnur Pushpakumar,Subir K Juin,Venkatakrishna R Jala,Utpal Sen

Circulation research 129:992-1005 PubMed34615369

2021

SMC-Derived Hyaluronan Modulates Vascular SMC Phenotype in Murine Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Felicia Hartmann,Daniel J Gorski,Alexandra A C Newman,Susanne Homann,Anne Petz,Katherine M Owsiany,Vlad Serbulea,Yu-Qing Zhou,Rebecca A Deaton,Michelle Bendeck,Gary K Owens,Jens W Fischer

American journal of physiology. Renal physiology 320:F1133-F1151 PubMed33870733

2021

Sacubitril/valsartan treatment has differential effects in modulating diabetic kidney disease in mice and KKAy mice compared with valsartan treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Komuraiah Myakala,Bryce A Jones,Xiaoxin X Wang,Moshe Levi

International journal of heart failure 3:59-68 PubMed36263109

2021

Endurance Exercise Training Prevents Elevation of Soluble ST2 in Mice with Doxorubicin-Induced Myocardial Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bong Joon Kim,Ji-Yeon Choi,Sun-Ju Oh,Jung-Ho Heo
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column

Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB119601

Mouse Lipocalin-2 ELISA Kit (NGAL)

elisa-kits

mouse-lipocalin-2-elisa-kit-ngal-ab119601

0

(0 reviews)

Alternative Product
Assay Kits

AB229420

Mouse Lipocalin-2 ELISA Kit, Fluorescent

elisa-kits

mouse-lipocalin-2-elisa-kit-fluorescent-ab229420

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com