Mouse Lipocalin-2 ELISA Kit (NGAL) is a single-wash 90-min Simplestep used to quantify Mouse Lipocalin-2 (NGAL) with a sensitivity of 1.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
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- Cancers 15:2023c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin Liu,Margaret R Smith,Yuezhu Wang,Ralph D'Agostino,Jimmy Ruiz,Thomas Lycan,Gregory L Kucera,Lance D Miller,Wencheng Li,Michael D Chan,Michael Farris,Jing Su,Qianqian Song,Dawen Zhao,Arvind Chandrasekaran,Fei XingPubMed 37174093
- Aging cell 22:e137862023Bifidobacterium longum supplementation improves age-related delays in fracture repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph L Roberts,Mateo Golloshi,Derek B Harding,Madison Conduah,Guanglu Liu,Hicham DrissiPubMed 36704918
- Neurobiology of disease 176:1059642022TNF and IL6/Jak2 signaling pathways are the main contributors of the glia-derived neuroinflammation present in Lafora disease, a fatal form of progressive myoclonus epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Rubio,Rosa Viana,Mireia Moreno-Estellés,Ángela Campos-Rodríguez,Pascual SanzPubMed 36526090
- Nature metabolism 4:995-10062022Systemic induction of senescence in young mice after single heterochronic blood exchange.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOk Hee Jeon,Melod Mehdipour,Tae-Hwan Gil,Minha Kang,Nicholas W Aguirre,Zachery R Robinson,Cameron Kato,Jessy Etienne,Hyo Gyeong Lee,Fatouma Alimirah,Vighnesh Walavalkar,Pierre-Yves Desprez,Michael J Conboy,Judith Campisi,Irina M ConboyPubMed 35902645
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- Nature communications 12:73332021A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Yao,Yalan Deng,Yang Zhao,Ying Mei,Yilei Zhang,Xiaoguang Liu,Consuelo Martinez,Xiaohua Su,Roberto R Rosato,Hongqi Teng,Qinglei Hang,Shannon Yap,Dahu Chen,Yumeng Wang,Mei-Ju May Chen,Mutian Zhang,Han Liang,Dong Xie,Xin Chen,Hao Zhu,Jenny C Chang,M James You,Yutong Sun,Boyi Gan,Li MaPubMed 34921145
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- Circulation research 129:992-10052021SMC-Derived Hyaluronan Modulates Vascular SMC Phenotype in Murine Atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelicia Hartmann,Daniel J Gorski,Alexandra A C Newman,Susanne Homann,Anne Petz,Katherine M Owsiany,Vlad Serbulea,Yu-Qing Zhou,Rebecca A Deaton,Michelle Bendeck,Gary K Owens,Jens W FischerPubMed 34615369
- American journal of physiology. Renal physiology 320:F1133-F11512021Sacubitril/valsartan treatment has differential effects in modulating diabetic kidney disease in mice and KKAy mice compared with valsartan treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKomuraiah Myakala,Bryce A Jones,Xiaoxin X Wang,Moshe LeviPubMed 33870733
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 4.69 - 300 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.1 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:15531878, PubMed:16446425). Can also bind siderophores from M.tuberculosis (By similarity).
Alternative names
Neutrophil gelatinase-associated lipocalin, NGAL, Lipocalin-2, Oncogene 24p3, SV-40-induced 24p3 protein, Siderocalin LCN2, p25, 24p3, Lcn2
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Recommended products
Mouse Lipocalin-2 ELISA Kit (NGAL) is a single-wash 90-min Simplestep used to quantify Mouse Lipocalin-2 (NGAL) with a sensitivity of 1.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 4.69 - 300 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 1.1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 3.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 109 | Range 106 - 110 % |
Sample type EDTA Plasma | Average % = 107 | Range 87 - 120 % |
Sample type Urine | Average % = 105 | Range 100 - 107 % |
Sample type Citrate plasma | Average % = 109 | Range 98 - 116 % |
Sample type Cell culture media | Average % = 104 | Range 102 - 107 % |
Sample type serum free media | Average % = 99 | Range 98 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lipocalin-2 (NGAL) protein in cit plasma, serum, urine, edta plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Lipocalin-2 (NGAL) with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity and renal development. Mice deficient in Lipocalin-2 appear normal but have increased susceptibility to bacterial infection. The bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Mouse Lipocalin-2 has 81% and 62% protein sequence identity to rat and human Lipocalin-2, respectively.
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5 product images
Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)
Mouse Lipocalin-2 (NGAL) standard curve comparison data.
Standard curve comparison between mouse Lipocalin-2 (NGAL) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 7-fold increase in sensitivity.
Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)
Example of Lipocalin-2 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)
Interpolated concentrations of Lipocalin-2 in mouse serum, plasma (citrate), and platelet poor plasma (EDTA).
The concentrations of Lipocalin-2 were measured in duplicate and interpolated from the Lipocalin-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 265.2 ng/mL in mouse serum, 583.8 ng/mL in mouse plasma (Citrate), and 535.2 ng/mL in platelet poor mouse plasma (EDTA).
Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)
Comparison of secreted Lipocalin-2 in media, unstimulated and PMA/PHA-stimulated J774A.1 cells.
J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Raw data from duplicate measurements are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)
Comparison of secreted Lipocalin-2 in mouse cell lines with or without stimulation.
L929 and J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. RAW264.7 were grown in the absence (unstimulated) or presence of LPS (stimulated) for 2 days. Cell Culture supernatant was diluted to within the range of the assay, 5-fold (L929 and J774A.1) or 2,000-fold (RAW264.7). Measured values were interpolated from the Lipocalin-2 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. Lipocalin-2 was undetectable in media.
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