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AB325903

Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent

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Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Lipocalin-2 (NGAL) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.27 - 600 pg/ml with a sensitivity of 0.17 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Neutrophil gelatinase-associated lipocalin, NGAL, Lipocalin-2, Oncogene 24p3, SV-40-induced 24p3 protein, Siderocalin LCN2, p25, 24p3, Lcn2

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Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)
  • sELISA

Lab

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)
  • sELISA

Lab

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)

Dynamic range comparison between the Chemiluminescent Mouse Lipocalin-2 SimpleStep ELISA, colorimetric Mouse Lipocalin-2 SimpleStep ELISA (ab199083), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)
  • sELISA

Lab

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)

Standard curve comparison between the Chemiluminescent Mouse Lipocalin-2 SimpleStep ELISA and colorimetric Mouse Lipocalin-2 SimpleStep ELISA (ab199083) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)

Comparison of secreted Lipocalin-2 in media, unstimulated and PMA/PHA-stimulated J774A.1 cells. J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Raw data from duplicate measurements are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)

Comparison of secreted Lipocalin-2 in mouse cell lines with or without stimulation. L929 and J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. RAW264.7 were grown in the absence (unstimulated) or presence of LPS (stimulated) for 2 days. Cell Culture supernatant was diluted to within the range of the assay, 5-fold (L929 and J774A.1) or 2,000-fold (RAW264.7). Measured values were interpolated from the Lipocalin-2 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. Lipocalin-2 was undetectable in media.

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (AB325903)

Interpolated concentrations of Lipocalin-2 in mouse serum, plasma (citrate), and platelet poor plasma (EDTA). The concentrations of Lipocalin-2 were measured in duplicate and interpolated from the Lipocalin-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 265.2 ng/mL in mouse serum, 583.8 ng/mL in mouse plasma (Citrate), and 535.2 ng/mL in platelet poor mouse plasma (EDTA).

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant, Urine, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.17 pg/mL

Range

0.27 - 600 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (ab325903) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Lipocalin-2 (NGAL) in Serum, Plasma – Citrate, Plasma – EDTA, Cell Culture Supernatant, Urine, Cell Culture Supernatant with a sensitivity of 0.17 pg/mL.

How the assay works

Mouse Lipocalin-2 (NGAL) ELISA Kit, Chemiluminescent (ab325903) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Lipocalin-2 (NGAL) (ab325903) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Lipocalin-2 (Lcn2) antibody [EPR17646-38] - BSA and Aside free (Capture) ab242493
- Anti-Lipocalin-2 (Lcn2) antibody [EPR17646-226] - BSA and Aside free (Detector) ab242742
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" } ]
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Recovery

[ { "sample": "Serum", "range": "106 - 110 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "98 - 116 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "87 - 120 %", "average": "= 107" }, { "sample": "Urine", "range": "100 - 107 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "102 - 107 %", "average": "= 104" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 15531878, PubMed : 16446425). Can also bind siderophores from M.tuberculosis (By similarity).
See full target information Lcn2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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