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AB326737

Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent

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Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse LIX (CXCL5) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.69 - 1500 pg/ml with a sensitivity of 0.23 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Scyb5, Cxcl5, C-X-C motif chemokine 5, Cytokine LIX, Small-inducible cytokine B5

6 Images
Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in mouse serum, and plasma samples.

The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 20.21 ng/mL in serum, 1.37 ng/mL in plasma (citrate), 3.49 ng/mL in plasma (EDTA), and 5.04 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in stimulated vs unstimulated mouse tissue culture supernatant samples.

The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 100%, LPS stimulated lung supernatant 1%, spleen supernatant 100%, and LPS stimulated spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 177.56 pg/mL in lung supernatant, 43.12 ng/mL LPS stimulated lung supernatant, 23.65 pg/mL spleen supernatant and 1250 pg/mL LPS stimulated spleen supernatant.

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in mouse LPS stimulated lung culture supernatant and LPS stimulated spleen culture supernatant samples.

The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : LPS stimulated lung culture supernatant 1% and LPS stimulated spleen culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 43.12 ng/mL in LPS stimulated lung supernatant and 1.25 ng/mL in LPS stimulated spleen supernatant.

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Interpolated concentrations of native LIX in mouse lung, spleen, and bone marrow based on a 500 μg/mL extract load.

The concentrations of LIX were measured in duplicate and interpolated from the LIX standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 571.73 pg/mL in lung extract, 548.73 pg/mL in spleen extract and 353.24 pg/mL in bone marrow extract.

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)
  • sELISA

Lab

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Dynamic range comparison between the Chemiluminescent Mouse LIX SimpleStep ELISA, colorimetric Mouse LIX SimpleStep ELISA (ab264611), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)
  • sELISA

Lab

Sandwich ELISA - Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (AB326737)

Standard curve comparison between the Chemiluminescent Mouse LIX SimpleStep ELISA and colorimetric Mouse LIX SimpleStep ELISA (ab264611) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Urine, Tissue Extracts, Heparin Plasma, Citrate plasma

Reacts with

Mouse, Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.23 pg/mL

Range

0.69 - 1500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (ab326737) is a rapid single-wash sandwich 55-min ELISA to measure Mouse LIX (CXCL5) in Cell culture supernatant, Serum, EDTA Plasma, Urine, Tissue Extracts, Heparin Plasma, Citrate plasma with a sensitivity of 0.23 pg/mL.

How the assay works

Mouse LIX (CXCL5) ELISA Kit, Chemiluminescent (ab326737) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse LIX (CXCL5) (ab326737) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-LIX antibody [EPR22392-249] - BSA and Azide free (Capture) ab267435
- Anti-LIX antibody [EPR22392-51] - BSA and Azide free (Detector) ab267436
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 103 %", "average": "= 103" }, { "sample": "Serum", "range": "80 - 110 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "91 - 109 %", "average": "= 109" }, { "sample": "Urine", "range": "87 - 89 %", "average": "= 89" }, { "sample": "Tissue Extracts", "range": "83 - 111 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "94 - 115 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "83 - 93 %", "average": "= 93" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.
See full target information Cxcl5
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com