Mouse LIX ELISA Kit (CXCL5) is a single-wash 90-min Simplestep used to quantify Mouse LIX (CXCL5) with a sensitivity of 1.14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Nature communications 13:76482022Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErik Klapproth,Anke Witt,Pauline Klose,Johanna Wiedemann,Nikitha Vavilthota,Stephan R Künzel,Susanne Kämmerer,Mario Günscht,David Sprott,Mathias Lesche,Fabian Rost,Andreas Dahl,Erik Rauch,Lars Kattner,Silvio Weber,Peter Mirtschink,Irakli Kopaliani,Kaomei Guan,Kristina Lorenz,Paul Saftig,Michael Wagner,Ali El-ArmouchePubMed 36496449
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.03 - 450 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.14 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.
Alternative names
Scyb5, Cxcl5, C-X-C motif chemokine 5, Cytokine LIX, Small-inducible cytokine B5
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Mouse LIX ELISA Kit (CXCL5) is a single-wash 90-min Simplestep used to quantify Mouse LIX (CXCL5) with a sensitivity of 1.14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.03 - 450 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.14 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 9.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 96 | Range 91 - 103 % |
Sample type Serum | Average % = 93 | Range 80 - 110 % |
Sample type EDTA Plasma | Average % = 101 | Range 91 - 109 % |
Sample type Urine | Average % = 88 | Range 87 - 89 % |
Sample type Tissue Extracts | Average % = 96 | Range 83 - 111 % |
Sample type Heparin Plasma | Average % = 100 | Range 94 - 115 % |
Sample type Citrate plasma | Average % = 88 | Range 83 - 93 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse LIX ELISA Kit (CXCL5) (ab264611) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LIX (CXCL5) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LIX (CXCL5) with 1.14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse LIX (LPS-induced CXC chemokine) also known as CXCL5, is a member of the ELR+ group of 7-9 kDa neutrophil and monocyte chemotactic proteins in the CXCL cytokine family. Mouse LIX can be produced by fibroblasts, epithelial cells (including alveolar type II epithelia), endothelial cells, platelets, cardiac myocytes, preosteoblasts, oligodendrocytes, and adipose-resident macrophages. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. Endotoxemia increases LIX expression, especially in the heart, but also in the lung, spleen, and liver. LIX is downregulated by glucocorticoids and is considered a glucocorticoid-attenuated response gene or GARG. It can also be downregulated by IL-10 and viral proteins. LIX is the most potent mouse neutrophil chemoattractant and activator LIX participates in the induction of LPS-induced acute lung injury and in lung ischemia-induced angiogenesis. High expression of LIX in obese white adipose tissue is thought to contribute to insulin resistance.Mouse CXCL5 is the equivalent of human CXCL6 based on genetic and biological evidence as pointed out by Nomiyama *et al.* (PMID: 20434943).
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCL5 also known as LIX (Liposaccharide-induced CXC chemokine) is a small cytokine belonging to the CXC chemokine family. It plays a significant role in immunological responses by acting as a chemoattractant for neutrophils. CXCL5 has a molecular mass of around 12 kDa. The protein is mainly expressed in neutrophils epithelial cells and endothelial cells. It is often studied alongside its competitors in different therapeutic and diagnostic contexts.
- Biological function summary
CXCL5 affects inflammation and the immune response by modulating neutrophil recruitment to sites of infection or injury. It is a part of the larger network of chemokines that direct leukocyte movement throughout the body. While CXCL5 does not form a complex in a traditional sense it does interact dynamically with CXCR2 receptor to exert its effects. These interactions are pivotal to the protein’s role in orchestrating the immune system’s response to diverse stimuli.
- Pathways
CXCL5 plays a part in the chemokine signaling pathway and the inflammatory response pathway. These pathways are important in mediating immune surveillance and inflammation. CXCL5 through these pathways has a strong connection to other CXC family proteins like CXCL1 and the receptor CXCR2 which allows for a coordinated response during immune challenges and recovery processes.
- Associated diseases and disorders
CXCL5 is relevant in the context of chronic inflammatory disorders such as rheumatoid arthritis and cancer specifically its role in tumor-related inflammation. In these conditions CXCL5 is linked to the exacerbation of inflammatory states and promotion of tumor growth via angiogenesis. The protein's interactions with CXCR2 play a significant role in these disease processes representing potential therapeutic targets for managing inflammation and modulating cancer progression.
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6 product images
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (ab264611)
Mouse LIX (CXCL5) standard curve comparison.
Standard Curve comparison between Mouse LIX (CXCL5) SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (ab264611)
Example of mouse LIX standard curve in Sample Diluent NS. The LIX standard curve was prepared as described in Section 10.
The LIX standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (ab264611)
Interpolated concentrations of native LIX in mouse serum, and plasma samples.
The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 20.21 ng/mL in serum, 1.37 ng/mL in plasma (citrate), 3.49 ng/mL in plasma (EDTA), and 5.04 ng/mL in plasma (heparin).
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (ab264611)
Interpolated concentrations of native LIX in mouse LPS stimulated lung culture supernatant and LPS stimulated spleen culture supernatant samples.
The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows: LPS stimulated lung culture supernatant 1% and LPS stimulated spleen culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 43.12 ng/mL in LPS stimulated lung supernatant and 1.25 ng/mL in LPS stimulated spleen supernatant.
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (ab264611)
Interpolated concentrations of native LIX in mouse lung, spleen, and bone marrow based on a 500 μg/mL extract load.
The concentrations of LIX were measured in duplicate and interpolated from the LIX standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 571.73 pg/mL in lung extract, 548.73 pg/mL in spleen extract and 353.24 pg/mL in bone marrow extract.
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (ab264611)
Interpolated concentrations of native LIX in stimulated vs unstimulated mouse tissue culture supernatant samples.
The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 100%, LPS stimulated lung supernatant 1%, spleen supernatant 100%, and LPS stimulated spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 177.56 pg/mL in lung supernatant, 43.12 ng/mL LPS stimulated lung supernatant, 23.65 pg/mL spleen supernatant and 1250 pg/mL LPS stimulated spleen supernatant.
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