Mouse LIX ELISA Kit (CXCL5)
- Recombinant
- SimpleStep
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(1 Publication)
Mouse LIX ELISA Kit (CXCL5) is a single-wash 90-min Simplestep used to quantify Mouse LIX (CXCL5) with a sensitivity of 1.14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Scyb5, Cxcl5, C-X-C motif chemokine 5, Cytokine LIX, Small-inducible cytokine B5
- sELISA
Supplier Data
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (AB264611)
Interpolated concentrations of native LIX in mouse LPS stimulated lung culture supernatant and LPS stimulated spleen culture supernatant samples.
The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : LPS stimulated lung culture supernatant 1% and LPS stimulated spleen culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 43.12 ng/mL in LPS stimulated lung supernatant and 1.25 ng/mL in LPS stimulated spleen supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (AB264611)
Interpolated concentrations of native LIX in mouse lung, spleen, and bone marrow based on a 500 μg/mL extract load.
The concentrations of LIX were measured in duplicate and interpolated from the LIX standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 571.73 pg/mL in lung extract, 548.73 pg/mL in spleen extract and 353.24 pg/mL in bone marrow extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (AB264611)
Mouse LIX (CXCL5) standard curve comparison.
Standard Curve comparison between Mouse LIX (CXCL5) SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (AB264611)
Interpolated concentrations of native LIX in stimulated vs unstimulated mouse tissue culture supernatant samples.
The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 100%, LPS stimulated lung supernatant 1%, spleen supernatant 100%, and LPS stimulated spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 177.56 pg/mL in lung supernatant, 43.12 ng/mL LPS stimulated lung supernatant, 23.65 pg/mL spleen supernatant and 1250 pg/mL LPS stimulated spleen supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (AB264611)
Interpolated concentrations of native LIX in mouse serum, and plasma samples.
The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 20.21 ng/mL in serum, 1.37 ng/mL in plasma (citrate), 3.49 ng/mL in plasma (EDTA), and 5.04 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (AB264611)
Example of mouse LIX standard curve in Sample Diluent NS. The LIX standard curve was prepared as described in Section 10.
The LIX standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity data
Product details
Mouse LIX ELISA Kit (CXCL5) (ab264611) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LIX (CXCL5) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LIX (CXCL5) with 1.14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse LIX (LPS-induced CXC chemokine) also known as CXCL5, is a member of the ELR+ group of 7-9 kDa neutrophil and monocyte chemotactic proteins in the CXCL cytokine family. Mouse LIX can be produced by fibroblasts, epithelial cells (including alveolar type II epithelia), endothelial cells, platelets, cardiac myocytes, preosteoblasts, oligodendrocytes, and adipose-resident macrophages. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. Endotoxemia increases LIX expression, especially in the heart, but also in the lung, spleen, and liver. LIX is downregulated by glucocorticoids and is considered a glucocorticoid-attenuated response gene or GARG. It can also be downregulated by IL-10 and viral proteins. LIX is the most potent mouse neutrophil chemoattractant and activator LIX participates in the induction of LPS-induced acute lung injury and in lung ischemia-induced angiogenesis. High expression of LIX in obese white adipose tissue is thought to contribute to insulin resistance.Mouse CXCL5 is the equivalent of human CXCL6 based on genetic and biological evidence as pointed out by Nomiyama *et al.* (PMID: 20434943).
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Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
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Recovery
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Properties and storage information
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Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CXCL5 affects inflammation and the immune response by modulating neutrophil recruitment to sites of infection or injury. It is a part of the larger network of chemokines that direct leukocyte movement throughout the body. While CXCL5 does not form a complex in a traditional sense it does interact dynamically with CXCR2 receptor to exert its effects. These interactions are pivotal to the protein’s role in orchestrating the immune system’s response to diverse stimuli.
Pathways
CXCL5 plays a part in the chemokine signaling pathway and the inflammatory response pathway. These pathways are important in mediating immune surveillance and inflammation. CXCL5 through these pathways has a strong connection to other CXC family proteins like CXCL1 and the receptor CXCR2 which allows for a coordinated response during immune challenges and recovery processes.
Product protocols
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Target data
Publications (1)
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Nature communications 13:7648 PubMed36496449
2022
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