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AB119603

Mouse LOX1 ELISA Kit (OLR1)

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(1 Publication)

Mouse LOX1 ELISA Kit (OLR1) is a Sandwich (quantitative) ELISA for the measurement of Mouse LOX1 (OLR1) in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Lox1, Olr1, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1

1 Images
Sandwich ELISA - Mouse LOX1 ELISA Kit (OLR1) (AB119603)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse LOX1 ELISA Kit (OLR1) (AB119603)

Representative Standard Curve using ab119603

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 1 pg/mL

Range

31.2 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's mouse LOX1 (OLR1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse LOX1 in cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA)

A LOX1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for LOX1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse LOX1 amount of sample captured in plate.

Get better reproducibility in only 90 minutes with Mouse LOX1 ELISA Kit (OLR1) (ab204521) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "750 pg/mL", "standardDeviation": "61.5", "coefficientOfVariability": "= 8.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "767 pg/mL", "standardDeviation": "60.59", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "85 pg/mL", "standardDeviation": "5.35", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "85 pg/mL", "standardDeviation": "4.84", "coefficientOfVariability": "= 8.2" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "312 pg/mL", "standardDeviation": "22.46", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "321 pg/mL", "standardDeviation": "17.33", "coefficientOfVariability": "= 5.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119603-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119603-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse LOX1 antibody", "number":"AB119603-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse LOX1 antibody Microplate (12 x 8 wells)", "number":"AB119603-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119603-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119603-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119603-CMP07", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119603-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119603-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse LOX1 standard", "number":"AB119603-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119603-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119603-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria (By similarity).
See full target information Olr1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 17:3595-3607 PubMed34512168

2021

Rutin attenuates Sorafenib-induced Chemoresistance and Autophagy in Hepatocellular Carcinoma by regulating BANCR/miRNA-590-5P/OLR1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhou,Gan Zhang,Jun Hu,Yanzhi Zhu,Haoming Lan,Xianfeng Shen,Yi Lv,Linsheng Huang
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websiteProtocolBooklet
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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