Mouse LOX1 ELISA Kit (OLR1)

Product details

Mouse LOX1 ELISA Kit (OLR1) (ab204521) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LOX1 (OLR1) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LOX1 (OLR1) with 25.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse Lectin like oxidized low density lipoprotein receptor 1 (LOX1), also known as oxidized low density lipoprotein receptor 1 (OLR-1), is a type II transmembrane receptor belonging to the C type lectin family. The mouse LOX1 gene encodes a 363 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, and a C-type lectin like domain which is required for ligand recognition. Mouse LOX1 has 57% and 79% identity to Human and Rat LOX1, respectively.

LOX1 is a receptor that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. The association of LOX1 with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, LOX1 acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Finally, LOX1 also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, and apoptotic cells.