AB199084

Mouse M-CSF ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Mouse M-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse M-CSF with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Csfm, Csf1, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF

6 Images

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

sELISA

Lab

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Mouse M-CSF standard curve comparison data.

Standard curve comparison between mouse M-CSF SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Linearity of dilution of the assay.

Titration of mouse serum, plasma (citrate) and stimulated L929 cell extracts. The 1X dilution for mouse serum was 6.25% matrix, and the 1X dilution for plasma was 50.0% matrix. The 1X dilution for the L929 extract was 500 μg/mL. The interpolated M-CSF concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

sELISA

Supplier Data

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Titration of Stimulated L929 supernatant and Unstimulated L929 supernatant within the working range of the assay.

The 1X dilution for stimulated L929 supernatant was 12.5% matrix, and the 1X dilution for unstimulated L929 supernatant was 25.0% matrix. The interpolated M-CSF concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

sELISA

Supplier Data

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Representative image of Mouse M-CSF ELISA Kit (ab199084)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Mouse M-CSF Detector Antibody

- Mouse M-CSF Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Mouse M-CSF Capture Antibody (Lyophilised) (2 vials)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Example of M-CSF standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Example of M-CSF standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 3.7 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse M-CSF ELISA Kit (ab199084) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of M-CSF protein in cell culture supernatant, serum, cit plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse M-CSF with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

M-CSF is a secreted cytokine that plays an essential role in the regulation of differentiation and maintenance of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. It plays an important role in innate immunity and in inflammatory processes where it acts to promote the release of proinflammatory chemokines. M-CSF also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Reports show that it is required for normal male and female fertility. It also acts in reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.28" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.35" } ]

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Recovery

[ { "sample": "Serum", "range": "92 - 105 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "107 - 112 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "83 - 90 %", "average": "= 86" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab199084-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB199084-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB199084-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB199084-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB199084-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB199084-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB199084-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse M-CSF Capture Antibody (lyophilized)", "number":"AB199084-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB199084-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse M-CSF Lyophilized Recombinant Protein", "number":"AB199084-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB199084-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB199084-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse M-CSF Detector Antibody", "number":"AB199084-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF also called CSF1 or macrophage colony-stimulating factor is a cytokine with a molecular mass of approximately 40 kDa. It plays a significant role in the regulation of macrophage production differentiation and function. M-CSF is expressed in various tissues including the bone marrow spleen and liver. The expression of this cytokine is pivotal for the development and survival of mononuclear phagocytes and is critical in myeloid lineage regulation.

Biological function summary

This cytokine functions as a growth factor that influences the survival proliferation and differentiation of hematopoietic progenitor cells into mature macrophages. M-CSF forms a homodimeric complex that interacts with its receptor c-Fms which is a cell surface glycoprotein expressed on monocytes and macrophages. This interaction is essential for signal transduction that leads to the activation of pathways involved in cell growth and proliferation. Unlike some other growth factors M-CSF does not require the presence of a co-factor to exert its effects on target cells.

Pathways

M-CSF is involved in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are critical for the mediation of cell proliferation and survival. Within these pathways M-CSF connects with other proteins such as AKT1 and MAPK1 which further transduce signals that aid in cellular growth and immune response regulation. M-CSF also interacts through its receptor c-Fms which propagates downstream signaling ensuring the appropriate activation of macrophage-related pathways.

An imbalance or dysregulation of M-CSF activity has been linked to disorders such as osteoporosis and certain cancers. Osteoporosis involves excessive bone resorption due to increased osteoclast activity where M-CSF participates in osteoclast differentiation. In cancer particularly breast and prostate cancer M-CSF expression supports tumor-associated macrophages promoting tumor progression. Additionally c-Fms is involved in these disease processes creating a significant therapeutic target for modulating M-CSF activity to potentially alleviate symptoms or halt disease progression.

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Product protocols

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Target data

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

See full target information Csf1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106470 PubMed37091244

2023

An inducible explant model of osteoclast-osteoprogenitor coordination in exacerbated osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jarred M Whitlock,Luis F de Castro,Michael T Collins,Leonid V Chernomordik,Alison M Boyce

Brain, behavior, and immunity 110:348-364 PubMed36940752

2023

Schwann cell insulin-like growth factor receptor type-1 mediates metastatic bone cancer pain in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenzo Landini,Matilde Marini,Daniel Souza Monteiro de Araujo,Antonia Romitelli,Marco Montini,Valentina Albanese,Mustafa Titiz,Alessandro Innocenti,Francesca Bianchini,Pierangelo Geppetti,Romina Nassini,Francesco De Logu

Journal of diabetes research 2018:2527406 PubMed29765986

2018

Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Jin,Liang Liu,Yaping Zhang,Yin Xiang,Guizhi Yin,Yi Lu,Ludong Shi,Jian Dong,Chengxing Shen

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse M-CSF ELISA Kit

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Sandwich ELISA - Mouse M-CSF ELISA Kit (AB199084)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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