AB289652

Mouse MAG/GMA ELISA Kit

Recombinant
SimpleStep
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Mouse MAG/GMA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MAG/GMA with a sensitivity of 9.285 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Myelin-associated glycoprotein, Siglec-4a, Mag

3 Images

Sandwich ELISA - Mouse MAG/GMA ELISA Kit (AB289652)

sELISA

Supplier Data

Sandwich ELISA - Mouse MAG/GMA ELISA Kit (AB289652)

Representative image of Mouse MAG/GMA ELISA Kit (ab289652)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse MAG/GMA ELISA Kit Capture Antibody

- 10X Mouse MAG/GMA Detector Antibody

- 10X Mouse MAG/GMA ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse MAG/GMA ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Mouse MAG/GMA ELISA Kit (AB289652)

Interpolated concentrations of mouse MAG/GMA in brain extracts.

Interpolated concentration of native MAG/GMA was measured in duplicate at different sample concentrations. Undiluted samples are 5,000 ng/mL mouse brain and rat brain. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1 Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse MAG/GMA ELISA Kit (AB289652)

Example of mouse MAG/GMA standard curve in 1X Cell Extraction Buffer PTR.

Example of mouse MAG/GMA standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 9.285 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MAG/GMA ELISA Kit (ab289652) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse MAG/GMA in cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MAG/GMA with 9.285 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": null } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "91 - 104 %", "average": "= 98" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289652-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289652-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289652-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB289652-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289652-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289652-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289652-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MAG/GMA Lyophilized Recombinant Protein", "number":"AB289652-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MAG/GMA Capture Antibody", "number":"AB289652-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB289652-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289652-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289652-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MAG/GMA Detector Antibody", "number":"AB289652-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAG (Myelin-Associated Glycoprotein) sometimes referred to as 'MAG protein' 'MAG RAT' or 'MAG/GMA' weighs approximately 100 kDa. This protein is prominently expressed in the central and peripheral nervous systems. MAG localizes to the periaxonal region of myelinated axons making it essential for proper neuronal function. It plays an important role in maintaining the stability and structure of myelin sheaths which are important for the efficient transmission of nerve impulses.

Biological function summary

This protein acts as an adhesion molecule that contributes to the myelination process. It exists as part of a molecular complex that interacts with other oligodendrocyte and Schwann cell proteins. This interaction is important for nerve regeneration and the expansion of the myelin sheath during development and repair. MAG also assists in signaling between axons and glial cells improving cellular communication and myelin integrity.

Pathways

MAG significantly impacts pathways such as the Nogo receptor which is associated with inhibiting axonal growth and regeneration. MAG interacts with the NOGO protein and other members of the Nogo receptor signaling pathway where it functions to regulate neuronal growth and regeneration. Furthermore MAG's interplay with NOGO receptor components contributes to limiting excessive sprouting of axons balancing growth and stability of the nervous system.

MAG is notably linked to neuropathies and multiple sclerosis. In neuropathies abnormal MAG expression can disrupt nerve conduction and lead to demyelinating conditions. Multiple sclerosis sees extensive involvement of MAG where its malfunction or reduced expression associates with the failure of remyelination processes. Additionally MAG's connection with proteins such as NOGO and other myelin-associated inhibitors implicates its role in the pathology of these disorders offering potential targets for therapeutic interventions.

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Product protocols

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Target data

Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (PubMed : 12089450, PubMed : 27922006, PubMed : 7533044). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination (PubMed : 10625334, PubMed : 7516497, PubMed : 9262180, PubMed : 9469574, PubMed : 9482781, PubMed : 9482783). Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2 (PubMed : 26335717). Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (PubMed : 15953602, PubMed : 19158290). Negative regulator of neurite outgrowth that inhibits axon longitudinal growth (PubMed : 12089450, PubMed : 19158290, PubMed : 27922006). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides (PubMed : 17640868). In cerebellar granule cells the inhibition is mediated via binding to neuronal gangliosides (PubMed : 17640868). In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides (By similarity). Inhibits axon outgrowth by binding to RTN4R (PubMed : 12089450). Preferentially binds to alpha-2,3-linked sialic acid (PubMed : 27922006, PubMed : 7533044). Binds ganglioside Gt1b (PubMed : 27922006).

See full target information Mag

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

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Mouse MAG/GMA ELISA Kit

Sandwich ELISA - Mouse MAG/GMA ELISA Kit (AB289652)

Sandwich ELISA - Mouse MAG/GMA ELISA Kit (AB289652)

Sandwich ELISA - Mouse MAG/GMA ELISA Kit (AB289652)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise