Mouse MAP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MAP2 with a sensitivity of 43.31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Publications
Filter by
- Science advances 9:eadd26712023CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCláudio Gouveia Roque,Kyung Min Chung,Ethan P McCurdy,Radhika Jagannathan,Lisa K Randolph,Krystal Herline-Killian,Jimena Baleriola,Ulrich HengstPubMed 36867706
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 43.31 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Alternative names
Mtap2, Map2, Microtubule-associated protein 2, MAP-2
What's included?
Recommended products
Mouse MAP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MAP2 with a sensitivity of 43.31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 43.31 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 93 | Range 90 - 99 % |
Sample type Serum | Average % = 84 | Range 82 - 87 % |
Sample type EDTA Plasma | Average % = 84 | Range 82 - 89 % |
Sample type Heparin Plasma | Average % = 91 | Range 93 - 101 % |
Sample type Citrate plasma | Average % = 84 | Range 81 - 86 % |
Sample type Tissue Extracts | Average % = 81 | Range 80 - 83 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse MAP2 ELISA Kit (ab253229) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MAP2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MAP2 with 43.31 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MAP2 (Microtubule-associated protein 2) is a neuronal protein that regulates the structure and stability of microtubules. It belongs to the MAP2/Tau family. Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Mouse MAP2 shares 80.2%, 92.3%, and 74.4% sequence homology with human, rat, and bovine, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Mouse MAP2 ELISA Kit (ab253229)
Example of mouse MAP2 standard curve in Sample Diluent NS.
The MAP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse MAP2 ELISA Kit (ab253229)
Example of mouse MAP2 standard curve in 1X Cell Extraction Buffer PTR.
The MAP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse MAP2 ELISA Kit (ab253229)
Interpolated concentrations of native MAP2 in mouse brain tissue based on a 15 μg/mL extract load.
The concentrations of MAP2 were measured in duplicate and interpolated from the MAP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MAP2 concentration was determined to be 3632.84 pg/mL in mouse brain tissue extract.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com