AB272473

Mouse MASP1 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Mouse MASP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MASP1 with a sensitivity of 29.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Crarf, Masp3, Masp1, Mannan-binding lectin serine protease 1, Complement factor MASP-3, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, Mannose-binding protein-associated serine protease, Ra-reactive factor serine protease p100, Serine protease 5, MASP-1, RaRF

3 Images

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

sELISA

Supplier Data

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

Interpolated concentrations of native MASP1 in mouse serum and plasma samples.

The concentrations of MASP1 were measured in duplicates, interpolated from the MASP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400, plasma (citrate) 1 : 400, and plasma (EDTA) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASP1 concentration was determined to be 902.1 ng/mL in serum, 416.2 ng/mL in plasma (citrate) and 779.9 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

Representative image of Mouse MASP1 ELISA Kit (ab272473)

Components shown from left to right :

- Sample diluent NS

- 10X Mouse MASP1 Capture Antibody

- 10X Mouse MASP1 Detector Antibody

- Mouse MASP1 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

sELISA

Supplier Data

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

Example of mouse MASP1 standard curve in Sample Diluent NS + 1X Enhancer.

The MASP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 29.3 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse MASP1 ELISA kit (ab272473) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse MASP1 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MASP1 with 29.3 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MASP1, encoded by the *Masp1* gene, is a serine protease and the most abundant protease of the lectin pathway. MASP1 plays a central role in complement activation. The binding of mannose binding lectin (MBL) and ficolins to sugar moieties triggers the lectin pathway and leads to the activation of MASP1 that will recognize and neutralize pathogens through patterns of sugar moieties. Studies have shown MASP1 to promote coagulation and take an active role in the inflammation process. Human, rat, cow, and monkey share 90%, 97%, 89%, and 90% sequence homology, respectively.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.09" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "82 - 106 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "109 - 110 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "78 - 92 %", "average": "= 90" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272473-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272473-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272473-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB272473-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272473-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272473-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272473-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB272473-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MASP1 Capture Antibody", "number":"AB272473-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MASP1 Lyophilized Recombinant Protein", "number":"AB272473-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MASP1 Detector Antibody", "number":"AB272473-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272473-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MASP1 protein also known as mannan-binding lectin serine protease 1 or MASP-1 is a serine protease with a mass of approximately 76 kDa. It is mainly expressed in the liver and is secreted into the blood. MASP1 is an important component of the lectin pathway of the complement system. This protein is an enzyme that circulates in the blood in an inactive form and becomes active when it binds to pathogen surfaces linked to mannose-binding lectins or ficolins.

Biological function summary

MASP1 plays a vital role in the innate immune system by activating the complement cascade. MASP1 works as part of a complex made up of pattern recognition molecules and other proteases. On binding to pathogens the complex undergoes conformational changes that activate MASP1. This activation leads to the cleavage of downstream substrates including MASP-2 and complement component C4 triggering a series of enzymatic reactions that enhance the pathogen clearance by opsonization and lysis.

Pathways

MASP1 is heavily involved in the lectin pathway one of the three activation pathways of the complement system. It shares functional ties with MASP-2 which continues the cascade after MASP1 activates the pathway. While the lectin pathway runs parallel to the classical pathway it is distinguished by the nature of its initiating molecules that recognize carbohydrate patterns on pathogen surfaces without needing antibodies. MASP1’s role is critical in facilitating the initial steps of this process which eventually leads to the activation of the terminal complement pathway components.

MASP1 shows connections to immune-related conditions such as systemic lupus erythematosus (SLE) and infectious diseases. Its dysregulation or deficiency can impact the complement system leading to immune complex diseases like SLE due to the failure in clearing immune complexes effectively. Furthermore MASP1’s association with MASP-2 also makes it relevant in studies focused on lectin pathway deficiencies that result in increased susceptibility to infections highlighting its significance in maintaining host immunity.

style

left-column

Product protocols

style

left-column

Target data

Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Also plays a role in development.

See full target information Masp1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e113975 PubMed37718683

2023

Infection and inflammation stimulate expansion of a CD74 Paneth cell subset to regulate disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Iyshwarya Balasubramanian,Sheila Bandyopadhyay,Juan Flores,Jared Bianchi-Smak,Xiang Lin,Haoran Liu,Shengxiang Sun,Natasha B Golovchenko,Yue Liu,Dahui Wang,Radha Patel,Ivor Joseph,Panan Suntornsaratoon,Justin Vargas,Peter Hr Green,Govind Bhagat,Stephen M Lagana,Wang Ying,Yi Zhang,Zhihan Wang,Wei Vivian Li,Sukhwinder Singh,Zhongren Zhou,George Kollias,Laura A Farr,Shannon N Moonah,Shiyan Yu,Zhi Wei,Edward M Bonder,Lanjing Zhang,Pawel R Kiela,Karen L Edelblum,Ronaldo Ferraris,Ta-Chiang Liu,Nan Gao

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MASP1 ELISA Kit

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Proteins & Peptides

Product promise