AB208343

Mouse MBL ELISA Kit

Recombinant
SimpleStep
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Mouse MBL ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MBL with a sensitivity of 19.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Mannose-binding protein C, MBP-C, Mannan-binding protein, RA-reactive factor P28A subunit, RARF/P28A, Mbl2

6 Images

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

sELISA

Supplier Data

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

The concentrations of MBL were measured in duplicates interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows : serum 1 : 83 plasma (citrate) 1 : 83 plasma (EDTA) 1 : 167 plasma (heparin) 1 : 167. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MBL concentration was determined to be 169.0 ng/mL in serum 116.5 ng/mL in plasma (citrate) 179.8 ng/mL in plasma (EDTA) and 148.8 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Example of mouse MBL standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Example of mouse MBL standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Interpolated concentrations of native MBL in mouse liver and testis extract samples.

The concentrations of MBL were measured in three different dilutions in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of MBL per mg of extract (mean +/- SD, n=3).

sELISA

Supplier Data

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Interpolated concentrations of native MBL in mouse liver extract samples based on a 500 μg/mL extract load.

The concentrations of MBL were measured in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 1.56 ng/mL in liver extract sample.

sELISA

Supplier Data

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Interpolated concentrations of native MBL in mouse liver supernatant samples.

Mouse liver cells were cultured for 5 days in a medium and supernatant sample was prepared. The concentrations of MBL were measured in duplicates, interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows : liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 4.3 ng/mL in liver supernatant.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 19.7 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MBL ELISA Kit (ab208343) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MBL protein in cell culture supernatant, serum, plasma, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MBL with 19.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MBL is a secreted calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]

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Recovery

[ { "sample": "Serum", "range": "75 - 86.8 %", "average": "= 79.5" }, { "sample": "Cell culture media", "range": "95.4 - 103.3 %", "average": "= 99.7" }, { "sample": "Citrate plasma", "range": "90.8 - 117.3 %", "average": "= 102.7" }, { "sample": "EDTA Plasma", "range": "74.1 - 102.3 %", "average": "= 94.9" }, { "sample": "Heparin Plasma", "range": "88.8 - 101.2 %", "average": "= 96.6" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.

Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.

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Product protocols

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Target data

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).

See full target information Mbl2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MBL ELISA Kit

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Sandwich ELISA - Mouse MBL ELISA Kit (AB208343)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Assay Kits

Assay Kits

Product promise