AB246547

Mouse MCP-5 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Mouse MCP-5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MCP-5 with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

2 Images

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

sELISA

Supplier Data

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

Interpolated concentrations of spiked MCP-5 in mouse serum, plasma (citrate) and lung supernatant samples.

The concentrations of MCP-5 were measured in duplicates and interpolated from the MCP-5 standard curves. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, and lung supernatant 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

Example of mouse MCP-5 standard curve in Sample Diluent NS.

The MCP-5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6 pg/mL

Range

27.56 - 1700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MCP-5 ELISA Kit (ab246547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP-5 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP-5 with 6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision & recovery information

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

Recovery

[ { "sample": "Cell culture supernatant", "range": "106 - 114 %", "average": "= 111" }, { "sample": "Serum", "range": "113 - 119 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "100 - 106 %", "average": "= 103" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246547-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB246547-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246547-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB246547-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246547-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246547-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246547-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246547-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MCP-5 Lyophilized Recombinant Protein", "number":"AB246547-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP-5 Capture Antibody", "number":"AB246547-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP-5 Detector Antibody", "number":"AB246547-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-5 also known as CCL12 is a chemokine protein that plays a role in immune response by recruiting and activating leukocytes. It has a molecular mass of approximately 12 kDa. This protein is expressed mainly in tissues related to the immune system including the spleen and liver. MCP-5 exhibits a sequence homology with other members of the C-C chemokine family and shares functional similarities with MCP-1.

Biological function summary

MCP-5 takes part in promoting the migration of monocytes and lymphocytes to sites of inflammation. This chemokine is not a part of a complex but interacts with specific receptors on target cells such as CCR2. Through these interactions MCP-5 helps mediate a range of immune responses enhancing both innate and adaptive immune activities. It also contributes to the clearance of pathogens by guiding leukocytes to infection sites.

Pathways

MCP-5 plays an important role in the chemokine signaling pathway and the inflammatory response pathway. Through chemokine signaling MCP-5 interacts with CCR2 facilitating the movement of immune cells to areas of tissue damage or infection. This regulation of cell traffic is essential for maintaining homeostasis and responding to pathological changes in tissues. Another protein connected to MCP-5 in these pathways is MCP-3 which shares overlapping functions in immune cell recruitment.

MCP-5 has been implicated in conditions such as asthma and rheumatoid arthritis. In these disorders MCP-5 contributes to the recruitment of inflammatory cells worsening symptoms. It also shows a connection with other proteins like MCP-1 which similarly aids in the movement of monocytes to inflammatory sites. The presence and activity of MCP-5 and its related proteins can therefore represent potential targets for therapeutic interventions in controlling excessive inflammatory responses.

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Product protocols

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Target data

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

See full target information Ccl12

Alternative Names

Mcp5, Scya12, Ccl12, C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12, MCP-5

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Oxidative medicine and cellular longevity 2021:9987219 PubMed34484568

2021

MiR-21-3p Inhibits Adipose Browning by Targeting FGFR1 and Aggravates Atrial Fibrosis in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-An Pan,Hao Lin,Jian-Ying Yu,Hui-Li Zhang,Jun-Feng Zhang,Chang-Qian Wang,Jun Gu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MCP-5 ELISA Kit

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

Sandwich ELISA - Mouse MCP-5 ELISA Kit (AB246547)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

Precision & recovery information

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise