Mouse MCP1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MCP1 in Mouse in Plasma, Cell culture supernatant, Serum samples.
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- Cell death & disease 13:5622022LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Jia,Sujuan Xu,Ting Ren,Tianyi Pan,Xiaoyan Wang,Yunlu Zhang,Zhouping Zou,Man Guo,Qi Zeng,Bo Shen,Xiaoqiang DingPubMed 35732633
- Experimental and therapeutic medicine 23:3032022Sevoflurane preconditioning prevents acute renal injury caused by ischemia-reperfusion in mice via activation of the Nrf2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Xi Wang,Zhen-Ru Zhao,Ying Bai,Ya-Xing Li,Xiao-Ning Gao,Sen Zhang,Yan-Bin SunPubMed 35340877
- JCI insight 7:2022DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCorina M Borza,Gema Bolas,Fabian Bock,Xiuqi Zhang,Favour C Akabogu,Ming-Zhi Zhang,Mark de Caestecker,Min Yang,Haichun Yang,Ethan Lee,Leslie Gewin,Agnes B Fogo,W Hayes McDonald,Roy Zent,Ambra PozziPubMed 34941574
- Journal of cellular and molecular medicine 26:1515-152920224-octyl Itaconate inhibits lipopolysaccharide (LPS)-induced osteoarthritis via activating Nrf2 signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingchen Zhang,Xiaohui Bai,Rongrong Wang,Hao Zhao,Lili Wang,Jingwen Liu,Ming Li,Zheng Chen,Zejun Wang,Lianxin Li,Dawei WangPubMed 35068055
- BioMed research international 2020:19839402020The Role of a Selective P2Y Receptor Antagonist, MRS2578, on the Formation of Angiotensin II-Induced Abdominal Aortic Aneurysms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Du et. al.PubMed 32382533
- Frontiers in endocrinology 11:912020A Novel Indoline Derivative Ameliorates Diabesity-Induced Chronic Kidney Disease by Reducing Metabolic Abnormalities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Permyakova et. al.PubMed 32218769
- International journal of molecular sciences 20:2019Effects of Farnesiferol B on Ischemia-Reperfusion-Induced Renal Damage, Inflammation, and NF-κB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Zhang et. al.PubMed 31842453
- Journal of cellular physiology 235:3362-33712019miR-218 regulates diabetic nephropathy via targeting IKK-β and modulating NK-κB-mediated inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMo Li,Qiushi Guo,Hanqing Cai,Haiyang Wang,Zhiming Ma,Xuan ZhangPubMed 31549412
- The EMBO journal 38:2019Cideb controls sterol-regulated ER export of SREBP/SCAP by promoting cargo loading at ER exit sites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Su et. al.PubMed 30858281
- European journal of nutrition 58:2315-23262018Gallic acid, a common dietary phenolic protects against high fat diet induced DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTahereh Setayesh et. al.PubMed 30039436
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2.74 - 2000 pg/mL
- Sensitivity
- < 3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed:29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed:29993042).
Alternative names
Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1
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Mouse MCP1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MCP1 in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2.74 - 2000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 3 pg/mL
- General recovery
- 94%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95.29 | Range 84 - 104 % |
Sample type Serum | Average % = 94.28 | Range 83 - 103 % |
Sample type Plasma | Average % = 92.79 | Range 82 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's MCP1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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3 product images
ELISA - Mouse MCP1 ELISA Kit (ab100721)
MCP1 measured in mouse lysates showing quantity (pg) per microgram of total protein. Values retrieved from 1:100 and 1:1000 dilutions.
Sandwich ELISA - Mouse MCP1 ELISA Kit (ab100721)
Representative standard curve using ab100721.
Sandwich ELISA - Mouse MCP1 ELISA Kit (ab100721)
Representative standard curve using ab100721.
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