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AB100721

Mouse MCP1 ELISA Kit

5

(2 Reviews)

|

(17 Publications)

Mouse MCP1 ELISA Kit is a Sandwich ELISA for the measurement of Mouse MCP1 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1

3 Images
Sandwich ELISA - Mouse MCP1 ELISA Kit (AB100721)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB100721)

Representative standard curve using ab100721.

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB100721)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB100721)

Representative standard curve using ab100721.

ELISA - Mouse MCP1 ELISA Kit (AB100721)
  • ELISA

Lab

ELISA - Mouse MCP1 ELISA Kit (AB100721)

MCP1 measured in mouse lysates showing quantity (pg) per microgram of total protein. Values retrieved from 1 : 100 and 1 : 1000 dilutions.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MCP1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 95.29" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 94.28" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 92.79" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100721-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100721-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100721-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse MCP1", "number":"AB100721-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100721-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100721-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100721-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100721-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MCP1 Standard (lyophilized)", "number":"AB100721-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MCP1 Microplate (12 x 8 wells)", "number":"AB100721-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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Product protocols

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed : 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed : 29993042).
See full target information Ccl2
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadt0504 PubMed40333987

2025

EYA3 regulation of NF-κB and CCL2 suppresses cytotoxic NK cells in the premetastatic niche to promote TNBC metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheera R Rosenbaum,Connor J Hughes,Kaiah M Fields,Stephen Connor Purdy,Annika L Gustafson,Arthur Wolin,Drake Hampton,Natasha M Shrivastava,Nicholas Turner,Etienne Danis,Christopher Ebmeier,Nicole Spoelstra,Jennifer Richer,Paul Jedlicka,James C Costello,Rui Zhao,Heide L Ford

Nature communications 16:2028 PubMed40016240

2025

Aspirin-responsive gene switch regulating therapeutic protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbo Huang,Ana Palma Teixeira,Ting Gao,Shuai Xue,Mingqi Xie,Martin Fussenegger

Cell death & disease 13:562 PubMed35732633

2022

LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Jia,Sujuan Xu,Ting Ren,Tianyi Pan,Xiaoyan Wang,Yunlu Zhang,Zhouping Zou,Man Guo,Qi Zeng,Bo Shen,Xiaoqiang Ding

Experimental and therapeutic medicine 23:303 PubMed35340877

2022

Sevoflurane preconditioning prevents acute renal injury caused by ischemia-reperfusion in mice via activation of the Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Xi Wang,Zhen-Ru Zhao,Ying Bai,Ya-Xing Li,Xiao-Ning Gao,Sen Zhang,Yan-Bin Sun

JCI insight 7: PubMed34941574

2022

DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Corina M Borza,Gema Bolas,Fabian Bock,Xiuqi Zhang,Favour C Akabogu,Ming-Zhi Zhang,Mark de Caestecker,Min Yang,Haichun Yang,Ethan Lee,Leslie Gewin,Agnes B Fogo,W Hayes McDonald,Roy Zent,Ambra Pozzi

Journal of cellular and molecular medicine 26:1515-1529 PubMed35068055

2022

4-octyl Itaconate inhibits lipopolysaccharide (LPS)-induced osteoarthritis via activating Nrf2 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingchen Zhang,Xiaohui Bai,Rongrong Wang,Hao Zhao,Lili Wang,Jingwen Liu,Ming Li,Zheng Chen,Zejun Wang,Lianxin Li,Dawei Wang

BioMed research international 2020:1983940 PubMed32382533

2020

The Role of a Selective P2Y Receptor Antagonist, MRS2578, on the Formation of Angiotensin II-Induced Abdominal Aortic Aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Du,Shilan Zhang,Qunyan Xiang,Jingyuan Chen,Feng Tian,Jin Xu,Xin Li,Yuansheng Tan,Ling Liu

Frontiers in endocrinology 11:91 PubMed32218769

2020

A Novel Indoline Derivative Ameliorates Diabesity-Induced Chronic Kidney Disease by Reducing Metabolic Abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Permyakova,Asaad Gammal,Liad Hinden,Michal Weitman,Marta Weinstock,Joseph Tam

International journal of molecular sciences 20: PubMed31842453

2019

Effects of Farnesiferol B on Ischemia-Reperfusion-Induced Renal Damage, Inflammation, and NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Xianjun Fu,Ting Gui,Tianqi Wang,Zhenguo Wang,Gerd A Kullak-Ublick,Zhibo Gai

Journal of cellular physiology 235:3362-3371 PubMed31549412

2019

miR-218 regulates diabetic nephropathy via targeting IKK-β and modulating NK-κB-mediated inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Li,Qiushi Guo,Hanqing Cai,Haiyang Wang,Zhiming Ma,Xuan Zhang
View all publications
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