AB100722

Mouse MCP1 ELISA Kit

Name: Mouse MCP1 ELISA Kit (ab100722) | Abcam
Brand: Abcam Limited
SKU: AB100722

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(8 Publications)

Mouse MCP1 ELISA Kit is a Sandwich ELISA for the measurement of Mouse MCP1 in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1

1 Images

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB100722)

sELISA

Unknown

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB100722)

Representative standard curve using ab100722

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's MCP1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP1 in cell lysates and tissue lysates.

This assay employs an antibody specific for mouse MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "80 - 102 %", "average": "= 87.56" }, { "sample": "Tissue Extracts", "range": "78 - 101 %", "average": "= 85.89" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100722-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100722-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100722-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100722-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100722-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100722-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100722-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MCP1 Standard (lyophilized)", "number":"AB100722-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MCP1 Microplate (12 x 8 wells)", "number":"AB100722-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse MCP1", "number":"AB100722-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100722-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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Product protocols

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed : 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed : 29993042).

See full target information Ccl2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell and tissue banking 25:805-830 PubMed38844606

2024

Effects of nanographene oxide on adipose-derived stem cell cryopreservation.

Applications

Unspecified application

Species

Unspecified reactive species

Zifei Li,Jun Qi,Su Fu,Jie Luan,Qian Wang

Immunity, inflammation and disease 11:e835 PubMed37647427

2023

TFRC in cardiomyocytes promotes macrophage infiltration and activation during the process of heart failure through regulating Ccl2 expression mediated by hypoxia inducible factor-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyun Pan,Jinxiu Yang,Jin Dai,Xiaoming Xu,Xinbin Zhou,Wei Mao

Journal of cell communication and signaling 17:897-914 PubMed36947363

2023

METTL3-METTL14 complex induces necroptosis and inflammation of vascular smooth muscle cells via promoting N6 methyladenosine mRNA methylation of receptor-interacting protein 3 in abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Dongbin Zhang,Shuiting Zhai,Huilin Wu,Hongzhi Liu

BMC nephrology 23:124 PubMed35354439

2022

METTL3 enhances NSD2 mRNA stability to reduce renal impairment and interstitial fibrosis in mice with diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Weiming Tang,Yilin Zhao,Hui Zhang,Ying Peng,Zhilian Rui

Phytotherapy research : PTR 36:1770-1784 PubMed35192202

2022

A novel natural PPARγ agonist, Gypenoside LXXV, ameliorates cognitive deficits by enhancing brain glucose uptake via the activation of Akt/GLUT4 signaling in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangbao Meng,Yuan Zhang,Zongyang Li,Jinxian Hu,Di Zhang,Weiwei Cao,Min Li,Guoxu Ma,Sicen Wang,Ping Cui,Qian Cai,Guodong Huang

Phytotherapy research : PTR 36:1297-1309 PubMed35088915

2022

A novel DPP-4 inhibitor Gramcyclin A attenuates cognitive deficits in APP/PS1/tau triple transgenic mice via enhancing brain GLP-1-dependent glucose uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Zongyang Li,Yuan Zhang,Xiangbao Meng,Min Li,Weiwei Cao,Junshan Yang,Xudong Xu,Wenlan Liu,Weiping Li,Qian Cai,Sicen Wang,Guoxu Ma,Zhiheng Liu,Guodong Huang

Antioxidants (Basel, Switzerland) 6: PubMed29104232

2017

Treatment with a Catalytic Superoxide Dismutase (SOD) Mimetic Improves Liver Steatosis, Insulin Sensitivity, and Inflammation in Obesity-Induced Type 2 Diabetes.

Applications

FuncS

Species

Mouse

Gina M Coudriet,Meghan M Delmastro-Greenwood,Dana M Previte,Meghan L Marré,Erin C O'Connor,Elizabeth A Novak,Garret Vincent,Kevin P Mollen,Sojin Lee,H Henry Dong,Jon D Piganelli

Antioxidants & redox signaling 28:1543-1561 PubMed29020797

2017

Extracellular Superoxide Dismutase Attenuates Renal Oxidative Stress Through the Activation of Adenosine Monophosphate-Activated Protein Kinase in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Ah Hong,Ji Hee Lim,Min Young Kim,Yaeni Kim,Hoon Suk Park,Hyung Wook Kim,Bum Soon Choi,Yoon Sik Chang,Hye Won Kim,Tae-Yoon Kim,Cheol Whee Park

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MCP1 ELISA Kit

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB100722)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise