Mouse MCP1 ELISA Kit ab208979 is a single-wash 90-min SimpleStep ELISA used to quantify Mouse MCP1 with a sensitivity of 0.487 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Mouse MCP1 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
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- Nature communications 14:55002023Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaela M Novakovic,Kirill S Korshunov,Rogan A Grant,Megan E Martin,Hiam A Valencia,G R Scott Budinger,Jelena Radulovic,Murali PrakriyaPubMed 37679321
- JVS-vascular science 4:1001242023Vascular smooth muscle cell mechanotransduction through serum and glucocorticoid inducible kinase-1 promotes interleukin-6 production and macrophage accumulation in murine hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMario Figueroa,SarahRose Hall,Victoria Mattia,Alex Mendoza,Adam Brown,Ying Xiong,Rupak Mukherjee,Jeffrey A Jones,William Richardson,Jean Marie RuddyPubMed 37920479
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23021302023Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S-Palmitoylated IRHOM2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Yi Zhang,Fufeng Gao,Xianling Dai,Qin Kuang,Jie Chai,Benkui Zou,Bochu WangPubMed 37544908
- Acta pharmaceutica Sinica. B 13:4234-42522023MANF brakes TLR4 signaling by competitively binding S100A8 with S100A9 to regulate macrophage phenotypes in hepatic fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Hou,Dong Wang,Mingxia Zhao,Petek Ballar,Xinru Zhang,Qiong Mei,Wei Wang,Xiang Li,Qiang Sheng,Jun Liu,Chuansheng Wei,Yujun Shen,Yi Yang,Peng Wang,Juntang Shao,Sa Xu,Fuyan Wang,Yang Sun,Yuxian ShenPubMed 37799387
- iScience 26:1069532023SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Xia,Meng Mao,Gaofeng Zhan,Zhenzhao Luo,Yin Zhao,Xing LiPubMed 37332598
- Allergologia et immunopathologia 51:135-1422023Neferine alleviates ovalbumin-induced asthma via MAPK signaling pathways in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTonggang Zhu,Xue Xiao,Yufu Dong,Chengbo YuanPubMed 37169571
- Microorganisms 11:2023Oral Administration of CRL1505 Modulates Lung Innate Immune Response against ST25.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefania Dentice Maidana,Yoshiya Imamura,Mariano Elean,Leonardo Albarracín,Keita Nishiyama,Yoshihito Suda,Shoichiro Kurata,María Ángela Jure,Haruki Kitazawa,Julio VillenaPubMed 37317122
- Biological procedures online 25:102023Exosomal microRNA-342-5p secreted from adipose-derived mesenchymal stem cells mitigates acute kidney injury in sepsis mice by inhibiting TLR9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liu,Chenghuan Hu,Buyao Zhang,Mingxia Li,Fuxing Deng,Shuangping ZhaoPubMed 37085762
- Acta pharmaceutica Sinica. B 13:1071-10922023The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis blocking inactive rhomboid protein 2-dependent pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Wei Dong,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Qiang Li,Deshuai Lou,Linfeng Hu,Xi Liu,Gang Kuang,Jing Luo,Jing Feng,Bochu WangPubMed 36970206
- Science advances 8:eabo46622022Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGwyneth M Welch,Carles A Boix,Eloi Schmauch,Jose Davila-Velderrain,Matheus B Victor,Vishnu Dileep,P Lorenzo Bozzelli,Qiao Su,Jemmie D Cheng,Audrey Lee,Noelle S Leary,Andreas R Pfenning,Manolis Kellis,Li-Huei TsaiPubMed 36170369
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.56 - 100 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.487 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed:29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed:29993042).
Alternative names
Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1
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Mouse MCP1 ELISA Kit ab208979 is a single-wash 90-min SimpleStep ELISA used to quantify Mouse MCP1 with a sensitivity of 0.487 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Mouse MCP1 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.56 - 100 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 0.487 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 8 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Mouse Serum | n 3 | mean - | SD - | C.V. 9.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 98 | Range 91 - 107 % |
Sample type Serum | Average % = 105 | Range 101 - 112 % |
Sample type EDTA Plasma | Average % = 106 | Range 104 - 110 % |
Sample type Heparin Plasma | Average % = 105 | Range 103 - 108 % |
Sample type Citrate plasma | Average % = 104 | Range 100 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse MCP1 ELISA Kit (ab208979) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP1 with 0.487 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MCP1 (CCL2) is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory stimuli. MCP1 is involved in the recruitment of monocytes and lymphocytes and in the regulation of Th1/Th2 lymphocyte differentiation.The standard is affinity purified.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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3 product images
Sandwich ELISA - Mouse MCP1 ELISA Kit (ab208979)
Mouse MCP1 standard curve comparison data.
Standard curve comparison between mouse MCP1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold increase in sensitivity.
Sandwich ELISA - Mouse MCP1 ELISA Kit (ab208979)
Interpolated concentrations of native MCP1 in mouse serum, plasma and cell culture supernatant samples.
The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and RAW264.7 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 157.7 pg/mL in serum, 125.7 pg/mL in plasma (citrate), 121.1 pg/mL in plasma (EDTA), and 277.2 pg/mL in RAW264.7 cell culture supernatant.
Sandwich ELISA - Mouse MCP1 ELISA Kit (ab208979)
Example of mouse MCP1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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