JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB208979

Mouse MCP1 ELISA Kit

5

(2 Reviews)

|

(39 Publications)

Mouse MCP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MCP1 with a sensitivity of 0.487 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 35 citations

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1

4 Images
ELISA - Mouse MCP1 ELISA Kit (AB208979)
  • ELISA

Supplier Data

ELISA - Mouse MCP1 ELISA Kit (AB208979)
Sandwich ELISA - Mouse MCP1 ELISA Kit (AB208979)
  • sELISA

Unknown

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB208979)

Mouse MCP1 standard curve comparison data.

Standard curve comparison between mouse MCP1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold increase in sensitivity.

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB208979)
  • sELISA

Unknown

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB208979)

Example of mouse MCP1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB208979)
  • sELISA

Unknown

Sandwich ELISA - Mouse MCP1 ELISA Kit (AB208979)

Interpolated concentrations of native MCP1 in mouse serum, plasma and cell culture supernatant samples.

The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and RAW264.7 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 157.7 pg/mL in serum, 125.7 pg/mL in plasma (citrate), 121.1 pg/mL in plasma (EDTA), and 277.2 pg/mL in RAW264.7 cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.487 pg/mL

Range

1.56 - 100 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse MCP1 ELISA Kit ab208979 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse MCP1 in cell culture supernatant, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 0.487 pg/mL.

How the assay works

Mouse MCP1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse MCP1 ELISA Kit ab208979 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse MCP1 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MCP1 antibody [EPR16662-261] - BSA and Azide free (Capture) ab242702
- Anti-MCP1 antibody [EPR16662-307] - BSA and Azide free (Detector) ab242702

MCP1 (CCL2) is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory stimuli. MCP1 is involved in the recruitment of monocytes and lymphocytes and in the regulation of Th1/Th2 lymphocyte differentiation.The standard is affinity purified.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 107 %", "average": "= 98" }, { "sample": "Serum", "range": "101 - 112 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "104 - 110 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "103 - 108 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "100 - 111 %", "average": "= 104" } ]
style
left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab208979-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB208979-CMP05" }, { "size":"2 x 1 Vial", "name":"Mouse MCP1 Lyophilized Recombinant Protein (ab9901)", "number":"AB208979-CMP02" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP1 Capture Antibody", "number":"AB208979-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB208979-CMP10" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB208979-CMP03" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB208979-CMP01" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB208979-CMP06" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP1 Detector Antibody", "number":"AB208979-CMP08" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB208979-CMP09" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB208979-CMP11" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab208979-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB208979-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB208979-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB208979-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Mouse MCP1 Capture Antibody", "number":"AB208979-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB208979-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB208979-CMP03" }, { "size":"2 x 10 Vial", "name":"Mouse MCP1 Lyophilized Recombinant Protein (ab9901)", "number":"AB208979-CMP02" }, { "size":"1 x 6000 µL", "name":"10X Mouse MCP1 Detector Antibody", "number":"AB208979-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB208979-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB208979-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab208979-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB208979-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MCP1 Lyophilized Recombinant Protein (ab9901)", "number":"AB208979-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB208979-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB208979-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB208979-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP1 Capture Antibody", "number":"AB208979-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB208979-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB208979-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MCP1 Detector Antibody", "number":"AB208979-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB208979-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
style
left-column

Product protocols

style
left-column

Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed : 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed : 29993042).
See full target information Ccl2
style
left-column

Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Behavioral and brain functions : BBF 21:19 PubMed40605012

2025

Gper1 inhibition exacerbates traumatic brain injury-induced neurological impairments in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Fei Xue,Ying-Xi Wu,Yun-Ze Zhang,Tian-Zhi Zhao

Journal of pharmaceutical analysis 15:101251 PubMed40521370

2025

Impact of FASN-enriched EVs on endothelial cell function in obstructive sleep apnea hypopnea syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Tian,Dan Zhang,Huaian Yang,Xiaoli Zhang,Shengqun Xu

BMJ open diabetes research & care 13: PubMed39842865

2025

Mechanism of TGIF1 on glycolipid metabolism disorders in mice with type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Fuyan Bai,Liping Zheng,Li Tao,Shikai Wang,Yuchen Li,Lijun Hou

Marine drugs 22: PubMed39728104

2024

Therapeutic Potential of Oligo-Fucoidan in Mitigating Peritoneal Dialysis-Associated Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Wei Chen,Mei-Yi Wu,Nai-Jen Huang,Mai-Szu Wu,Yung-Ho Hsu,Chia-Te Liao,Cheng-Hsien Chen

World journal of gastroenterology 30:3689-3704 PubMed39193000

2024

Role of gut microbiota in Crohn's disease pathogenesis: Insights from fecal microbiota transplantation in mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Wu,Lian-Wen Yuan,Li-Chao Yang,Ya-Wei Zhang,Heng-Chang Yao,Liang-Xin Peng,Bao-Jia Yao,Zhi-Xian Jiang

Fundamental & clinical pharmacology 38:958-966 PubMed39009501

2024

Bezafibrate mitigates oxidized-low density lipoprotein (ox-LDL)-induced the attachment of monocytes to endothelial cells: An implication in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun Huang,Yan Shen

Nature aging 4:926-938 PubMed38816549

2024

SGLT2 inhibition eliminates senescent cells and alleviates pathological aging.

Applications

Unspecified application

Species

Unspecified reactive species

Goro Katsuumi,Ippei Shimizu,Masayoshi Suda,Yohko Yoshida,Takaaki Furihata,Yusuke Joki,Chieh-Lun Hsiao,Liang Jiaqi,Shinya Fujiki,Manabu Abe,Masataka Sugimoto,Tomoyoshi Soga,Tohru Minamino

The Journal of biological chemistry 300:107372 PubMed38754781

2024

Cardioprotective properties of OMT-28, a synthetic analog of omega-3 epoxyeicosanoids.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Kranrod,Anne Konkel,Robert Valencia,Ahmed M Darwesh,Robert Fischer,Wolf-Hagen Schunck,John M Seubert

Molecular medicine (Cambridge, Mass.) 30:59 PubMed38745316

2024

Polyphyllin I alleviates neuroinflammation after cerebral ischemia-reperfusion injury via facilitating autophagy-mediated M2 microglial polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Kang,Qiuling Sang,Dingxi Liu,Libo Wang,Jia Li,Xiaoyang Liu

Nutrients 16: PubMed38257186

2024

Improving the Effect of Ferulic Acid on Inflammation and Insulin Resistance by Regulating the JNK/ERK and NF-κB Pathways in TNF-α-Treated 3T3-L1 Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Eun Park,Ji-Sook Han
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB208348

Mouse TNF alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

  • 4
  • 4
Assay Kits

AB197742

Mouse IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

  • 5
  • 3
Assay Kits

AB222503

Mouse IL-6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com