Mouse MCP2 ELISA Kit (CCL8) is a single-wash 90-min Simplestep used to quantify Mouse MCP2 (CCL8) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Mcp2, Scya8, Ccl8, C-C motif chemokine 8, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2
- sELISA
Supplier Data
Sandwich ELISA - Mouse MCP2 ELISA Kit (CCL8) (AB203366)
Titration of mouse stimulated lung supernatant, unstimulated lung supernatant, stimulated spleen supernatant, and unstimulated spleen supernatant.
Titration of mouse stimulated (100 ng/mL LPS + 50 ng/mL PMA, 3 days (+)) lung supernatant, unstimulated (-) lung supernatant, stimulated (5 μg/mL LPS, 6 Days (+)) spleen supernatant, and unstimulated (-) spleen supernatant. A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 10.0% for the stimulated supernatants, and 20.0% for the unstimulated supernatants. Interpolated data values (mean +/- SD, n = 2) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MCP2 ELISA Kit (CCL8) (AB203366)
Example of MCP2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MCP2 ELISA Kit (CCL8) (AB203366)
Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.
The 1X dilution was 0.025% for the mouse serum and all of the mouse plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MCP2 ELISA Kit (CCL8) (AB203366)
Example of MCP2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Mouse MCP2 ELISA Kit (CCL8) (ab203366) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP2 (CCL8) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP2 (CCL8) with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MCP2 is a chemotactic factor that attracts monocytes. Chemokines and their receptors, in conjunction with adhesion molecules and their ligands, provide cues to enable tissue-specific homing of T cells in the steady state and during inflammation. Mouse MCP specifically induces migration of a population of recently activated, highly differentiated TH2 cells enriched in interleukin 5 (IL-5) and the IL-25 receptor (IL-25R), cytokines implicated in eosinophilic inflammation; as well as tumor necrosis factor (TNF) and OX40, molecules associated with inflammatory TH2 cells. Using a model of atopic dermatitis, we demonstrate a pathologic role for CCR8 and mouse CCL8, but not for the TH2-associated chemokine receptor CCR4 or the previously known mouse CCR8 chemokine ligand mouse CCL1 (TCA3), in mediating chronic cutaneous allergic inflammation.
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Supplementary information
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Biological function summary
MCP2 influences the migration and activation of leukocytes enhancing the body's immune response. MCP2 interacts with chemokine receptors like CCR2 and CCR5 facilitating the movement of immune cells through tissues. This protein is not usually part of larger complexes but works closely with other chemokines to coordinate cellular responses during inflammation. MCP2 activity is important in processes that require rapid cell recruitment and immune surveillance.
Pathways
MCP2 functions within the chemokine signaling pathway and plays a significant part in the inflammatory response pathway. MCP2 closely interacts with chemokine receptors such as CCR2 which is also engaged by related chemokines like MCP1 (CCL2). MCP2 and MCP1 share functional similarities in modulating the immune response though they target different receptor profiles that expand their influence across numerous immune processes.
Product protocols
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Publications (1)
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Biology 9: PubMed32938018
2020
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