Mouse MCP3 ELISA Kit (CCL7) is a single-wash 90-min Simplestep used to quantify Mouse MCP3 (CCL7) with a sensitivity of 1.53 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Carcinogenesis 43:647-6582022Immunosuppressive tumor microenvironment in uterine serous carcinoma via CCL7 signal with myeloid-derived suppressor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuka Mise,Junzo Hamanishi,Takiko Daikoku,Shiro Takamatsu,Taito Miyamoto,Mana Taki,Koji Yamanoi,Ken Yamaguchi,Masayo Ukita,Naoki Horikawa,Kaoru Abiko,Ryusuke Murakami,Yoko Furutake,Yuko Hosoe,Jumpei Terakawa,Masahiro Kagabu,Tamotsu Sugai,Mitsumasa Osakabe,Hiroshi Fujiwara,Noriomi Matsumura,Masaki Mandai,Tsukasa BabaPubMed 35353883
- Cellular and molecular life sciences : CMLS 79:1552022ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun-Jung Kim,Woo-Jin Kim,Hye-Rim Shin,Hee-In Yoon,Jae-I Moon,Eunji Lee,Jin-Muk Lim,Young-Dan Cho,Mi-Hye Lee,Hong-Gee Kim,Hyun-Mo RyooPubMed 35218410
- Redox biology 46:1020792021Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Kong,Wenhui Dong,Yuwen Zhu,Zhiwen Fan,Xiulian Miao,Yan Guo,Chengping Li,Yunfei Duan,Yunjie Lu,Zilong Li,Yong XuPubMed 34454163
- Journal of cellular and molecular medicine 25:7280-72932021CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCuiping Xie,Feiming Ye,Ning Zhang,Yuxue Huang,Yun Pan,Xiaojie XiePubMed 34189838
- Autophagy 17:4062-40822021Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Li,Mouli Tian,Tong Hua,Haowei Wang,Mei Yang,Wenqian Li,Xiaoping Zhang,Hongbin YuanPubMed 33834930
- mBio 11:2020Trypanosoma cruzi Induces the PARP1/AP-1 Pathway for Upregulation of Metalloproteinases and Transforming Growth Factor β in Macrophages: Role in Cardiac Fibroblast Differentiation and Fibrosis in Chagas Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubhadip Choudhuri et. al.PubMed 33172999
- Journal of cellular physiology 235:10094-101082020Helicobacter pylori promote inflammation and host defense through the cagA-dependent activation of mTORC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang-Jing Feng,Yi Chen,Ke LiPubMed 32722876
- Molecular therapy. Nucleic acids 22:273-2842020MicroRNA-410-3p Binds to TLR2 and Alleviates Myocardial Mitochondrial Dysfunction and Chemokine Production in LPS-Induced Sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTongkun Zuo et. al.PubMed 33230433
- Cell death & disease 10:5442019Hypoxia inducible factor 1α in vascular smooth muscle cells promotes angiotensin II-induced vascular remodeling via activation of CCL7-mediated macrophage recruitment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Qi,Ming Wei,Shiyu Jiao,Yanting Song,Xia Wang,Guomin Xie,Joseph Taranto,Ye Liu,Yan Duan,Baoqi Yu,Huihua Li,Yatrik M Shah,Qingbo Xu,Jie Du,Frank J Gonzalez,Aijuan QuPubMed 31320613
- JCI insight 1:e889472016Epithelial-macrophage interactions determine pulmonary fibrosis susceptibility in Hermansky-Pudlak syndrome.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.53 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity).
Alternative names
Fic, Mcp3, Scya7, Ccl7, C-C motif chemokine 7, Intercrine/chemokine MARC, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, Protein FIC, Small-inducible cytokine A7, MCP-3
What's included?
Recommended products
Mouse MCP3 ELISA Kit (CCL7) is a single-wash 90-min Simplestep used to quantify Mouse MCP3 (CCL7) with a sensitivity of 1.53 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.53 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 3.24 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 2.58 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 91 - 99 % |
Sample type Cell culture media | Average % = 89 | Range 89 - 91 % |
Sample type Citrate plasma | Average % = 98 | Range 94 - 102 % |
Sample type EDTA Plasma | Average % = 93 | Range 88 - 96 % |
Sample type Heparin Plasma | Average % = 85 | Range 80 - 87 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse MCP3 ELISA Kit (CCL7) (ab205571) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP3 (CCL7) protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP3 (CCL7) with 1.53 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MCP3, also known as CCL7, belongs to the C-C chemokine family. MCP3 attracts monocytes, eosinophils, NK cells, T-lymphocytes, but not neutrophils. The ability of MCP3 to interact with various immune cell types are explained by the molecule’s ability to interact with various chemokine receptors. The chemokines known to interact with MCP3 include CCR1, CCR2, and CCR3. MCP3 also augments monocyte anti-tumor activity, and has been implicated in various studies investigating cancer metastasis.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target MCP-3 also referred to as CCL7 is a chemotactic cytokine belonging to the CC chemokine family. Its molecular weight is approximately 11 kDa. MCP-3 is largely expressed by various cell types including monocytes lymphocytes and endothelial cells. This protein plays an important role in immune system signaling. MCP-3 exhibits chemotactic activity for monocytes T lymphocytes and natural killer (NK) cells contributing to immune cell recruitment at sites of inflammation.
- Biological function summary
MCP-3 interacts with several chemokine receptors such as CCR1 CCR2 and CCR3 allowing for diverse roles in immune response and inflammation. It functions as both a monomer and in conjunction with other chemokines to form heterodimers aiding in the modulation and fine-tuning of the immune response. The presence of MCP-3 in inflammatory tissues indicates its contribution to directing leukocyte migration and positioning therefore supporting both innate and adaptive immunity.
- Pathways
MCP-3 is a component of the chemokine signaling pathway and is significant in the regulation of immune surveillance. It interacts closely with proteins such as CCL2 and CCL3 all of which bind to similar receptors like CCR2 and CCR5. MCP-3's involvement in these pathways supports cellular responses to inflammation and infection and its activity has a direct impact on the mobilization and activation of immune cells.
- Associated diseases and disorders
MCP-3 shows associations with conditions marked by inflammation and immune system dysregulation including rheumatoid arthritis and asthma. The protein's influence in these disorders links to other chemokines like MCP-1 (CCL2) which also recruits monocytes and contributes to chronic inflammation. Disruption in MCP-3 expression or function can aggravate disease states by altering the balance of leukocyte trafficking and exacerbating inflammatory responses.
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3 product images
Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (ab205571)
Example of mouse MCP3 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (ab205571)
Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.
The 1X dilution was 25% for the mouse serum and all of the mouse plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (ab205571)
Titration of mouse stimulated (5 µg/mL LPS, 48 hrs (+)) and unstimulated (-) Raw 264.7 supernatant, and unstimulated (-) D10.G4.1 supernatant.
A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 3.13% for the stimulated and unstimulated Raw 264.7 supernatants, and 25.0% for the unstimulated D10.G4.1 supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.
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