AB205571

Mouse MCP3 ELISA Kit (CCL7)

Recombinant
SimpleStep
What is this?

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(11 Publications)

Mouse MCP3 ELISA Kit (CCL7) is a single-wash 90-min Simplestep used to quantify Mouse MCP3 (CCL7) with a sensitivity of 1.53 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Fic, Mcp3, Scya7, Ccl7, C-C motif chemokine 7, Intercrine/chemokine MARC, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, Protein FIC, Small-inducible cytokine A7, MCP-3

4 Images

ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

ELISA

Supplier Data

ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

sELISA

Supplier Data

Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.

The 1X dilution was 25% for the mouse serum and all of the mouse plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

Titration of mouse stimulated (5 µg/mL LPS, 48 hrs (+)) and unstimulated (-) Raw 264.7 supernatant, and unstimulated (-) D10.G4.1 supernatant.

A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 3.13% for the stimulated and unstimulated Raw 264.7 supernatants, and 25.0% for the unstimulated D10.G4.1 supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

Example of mouse MCP3 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.53 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MCP3 ELISA Kit (CCL7) ab205571 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse MCP3 (CCL7)in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 1.53 pg/mL.

How the assay works

Mouse MCP3 (CCL7)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse MCP3 ELISA Kit (CCL7) ab205571 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

MCP3, also known as CCL7, belongs to the C-C chemokine family. MCP3 attracts monocytes, eosinophils, NK cells, T-lymphocytes, but not neutrophils. The ability of MCP3 to interact with various immune cell types are explained by the molecule’s ability to interact with various chemokine receptors. The chemokines known to interact with MCP3 include CCR1, CCR2, and CCR3. MCP3 also augments monocyte anti-tumor activity, and has been implicated in various studies investigating cancer metastasis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.58" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.24" } ]

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Recovery

[ { "sample": "Serum", "range": "91 - 99 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "89 - 91 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "94 - 102 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "88 - 96 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "80 - 87 %", "average": "= 85" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target MCP-3 also referred to as CCL7 is a chemotactic cytokine belonging to the CC chemokine family. Its molecular weight is approximately 11 kDa. MCP-3 is largely expressed by various cell types including monocytes lymphocytes and endothelial cells. This protein plays an important role in immune system signaling. MCP-3 exhibits chemotactic activity for monocytes T lymphocytes and natural killer (NK) cells contributing to immune cell recruitment at sites of inflammation.

Biological function summary

MCP-3 interacts with several chemokine receptors such as CCR1 CCR2 and CCR3 allowing for diverse roles in immune response and inflammation. It functions as both a monomer and in conjunction with other chemokines to form heterodimers aiding in the modulation and fine-tuning of the immune response. The presence of MCP-3 in inflammatory tissues indicates its contribution to directing leukocyte migration and positioning therefore supporting both innate and adaptive immunity.

Pathways

MCP-3 is a component of the chemokine signaling pathway and is significant in the regulation of immune surveillance. It interacts closely with proteins such as CCL2 and CCL3 all of which bind to similar receptors like CCR2 and CCR5. MCP-3's involvement in these pathways supports cellular responses to inflammation and infection and its activity has a direct impact on the mobilization and activation of immune cells.

MCP-3 shows associations with conditions marked by inflammation and immune system dysregulation including rheumatoid arthritis and asthma. The protein's influence in these disorders links to other chemokines like MCP-1 (CCL2) which also recruits monocytes and contributes to chronic inflammation. Disruption in MCP-3 expression or function can aggravate disease states by altering the balance of leukocyte trafficking and exacerbating inflammatory responses.

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Product protocols

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Target data

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity).

See full target information Ccl7

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Environment & health (Washington, D.C.) 3:392-401 PubMed40270526

2025

Astrocytes Respond to Environment-Relevant Doses TCDD in a Specific Manner Which Is Different from the Endogenous AhR Ligand (FICZ).

Applications

Unspecified application

Species

Unspecified reactive species

Rui Sha,Yangsheng Chen,Tong Xu,Guanglei Yang,Li Xu,Heidi Qunhui Xie,Bin Zhao

Carcinogenesis 43:647-658 PubMed35353883

2022

Immunosuppressive tumor microenvironment in uterine serous carcinoma via CCL7 signal with myeloid-derived suppressor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Mise,Junzo Hamanishi,Takiko Daikoku,Shiro Takamatsu,Taito Miyamoto,Mana Taki,Koji Yamanoi,Ken Yamaguchi,Masayo Ukita,Naoki Horikawa,Kaoru Abiko,Ryusuke Murakami,Yoko Furutake,Yuko Hosoe,Jumpei Terakawa,Masahiro Kagabu,Tamotsu Sugai,Mitsumasa Osakabe,Hiroshi Fujiwara,Noriomi Matsumura,Masaki Mandai,Tsukasa Baba

Cellular and molecular life sciences : CMLS 79:155 PubMed35218410

2022

ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Jung Kim,Woo-Jin Kim,Hye-Rim Shin,Hee-In Yoon,Jae-I Moon,Eunji Lee,Jin-Muk Lim,Young-Dan Cho,Mi-Hye Lee,Hong-Gee Kim,Hyun-Mo Ryoo

Redox biology 46:102079 PubMed34454163

2021

Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Kong,Wenhui Dong,Yuwen Zhu,Zhiwen Fan,Xiulian Miao,Yan Guo,Chengping Li,Yunfei Duan,Yunjie Lu,Zilong Li,Yong Xu

Journal of cellular and molecular medicine 25:7280-7293 PubMed34189838

2021

CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiping Xie,Feiming Ye,Ning Zhang,Yuxue Huang,Yun Pan,Xiaojie Xie

Autophagy 17:4062-4082 PubMed33834930

2021

Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Mouli Tian,Tong Hua,Haowei Wang,Mei Yang,Wenqian Li,Xiaoping Zhang,Hongbin Yuan

mBio 11: PubMed33172999

2020

Trypanosoma cruzi Induces the PARP1/AP-1 Pathway for Upregulation of Metalloproteinases and Transforming Growth Factor β in Macrophages: Role in Cardiac Fibroblast Differentiation and Fibrosis in Chagas Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Subhadip Choudhuri,Nisha Jain Garg

Journal of cellular physiology 235:10094-10108 PubMed32722876

2020

Helicobacter pylori promote inflammation and host defense through the cagA-dependent activation of mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Guang-Jing Feng,Yi Chen,Ke Li

Molecular therapy. Nucleic acids 22:273-284 PubMed33230433

2020

MicroRNA-410-3p Binds to TLR2 and Alleviates Myocardial Mitochondrial Dysfunction and Chemokine Production in LPS-Induced Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Tongkun Zuo,Qing Tang,Xiangcheng Zhang,Futai Shang

Cell death & disease 10:544 PubMed31320613

2019

Hypoxia inducible factor 1α in vascular smooth muscle cells promotes angiotensin II-induced vascular remodeling via activation of CCL7-mediated macrophage recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Qi,Ming Wei,Shiyu Jiao,Yanting Song,Xia Wang,Guomin Xie,Joseph Taranto,Ye Liu,Yan Duan,Baoqi Yu,Huihua Li,Yatrik M Shah,Qingbo Xu,Jie Du,Frank J Gonzalez,Aijuan Qu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MCP3 ELISA Kit (CCL7)

ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

Sandwich ELISA - Mouse MCP3 ELISA Kit (CCL7) (AB205571)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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